“POLICE – TO PROTECT AND SERVE” ONLINE: FACTORS CONTRIBUTING TO MODERN WAYS OF INTERACTION BETWEEN CITIZENS AND LAW ENFORCEMENT AGENCIES

Web-based communication channels, like social networks, allow a bidirectional interaction be-tween governmental agencies like the police and citizens. The police clearly value the fact that they can reach a vast number of people within a very short time. Existing studies mainly focus on the police’s perspective. However, it is unclear what kinds of obstacles prevent citizens from using these new communication channels to help the police and what factors drive the usage. This study is one of the first to examine factors contributing to online citizen-police interactions. Applying the grounded theory methodology, we propose a model that reflects the following three classes: obstacles, enablers, and attitudes, all of which influence the interaction between state agencies and citizens. On the one hand, our results highlight that the fear of losing anonymity or the image of an omnipresent police can be seen as the main obstacles. On the other hand, improvements regarding the usability like adding a filtering function can increase the willing-ness to interact with the police on the side of the citizens. However, not all aspects can be influ-enced. Citizens’ attitudes which explain their personal impetus to engage in such interactions are virtually impossible to be changed by the police

Data from: Orientation of native versus translocated juvenile Lesser Spotted Eagles (Clanga pomarina) on the first autumn migration

The ontogeny of migration routines used by wild birds remains unresolved. Here we investigated the migratory orientation of juvenile lesser spotted eagles (LSE) (Clanga pomarina) based on translocation and satellite tracking. Between 2004 and 2016, 85 second-hatched juveniles (Abels) were reared in captivity for release into the declining German population, including 50 birds that were translocated 940 km from Latvia. In 2009, we tracked 12 translocated juveniles, as well as 8 native juveniles and 9 native adults, to determine how inexperienced birds come to use strategic migration routes. Native juveniles departed around the same time as the adults and 6 of 8 used the eastern flyway around the Mediterranean, which was used by all adults. In contrast, translocated juveniles departed on average 6 days before native LSEs, and 5 traveled southward and died in the central Mediterranean region. Consequently fewer translocated juveniles (4/12) than native juveniles (7/8) reached Africa. We conclude that juvenile LSEs have a much better chance of learning the strategic southeastern flyway if they leave at an appropriate time to connect with experienced elders upon departure. It is not clear why translocated juveniles departed so early. Regardless, by the end of the year, most juveniles had perished, whether they were translocated (10/12) or not (6/8). The small number of surviving translocated juveniles thus still represents a significant increase in the annual productivity of the German LSE population in 2009

Satellite tracking data of juvenile and adult eagles

Lesser Spotted Eagles were tracked by means of GPS-enhanced satellite telemetry (Microwave Telemetry, Inc., Columbia, MD, USA) during their autumn migration until the end of the year in 2009. Migration data were obtained for 11 juvenile Lesser Spotted Eagles after they were translocated from Latvia to Germany as well as for 8 juveniles and 9 adults native to the German population. The file contains the columns 'Bird' containing the number of the tracking device, 'Origin' showing whether the bird originated from Latvia ('L') or Germany ('GE'), 'Age' indicating whether the bird was tagged as an adult or as a juvenile, and the columns 'Latitude', 'Longitude', 'Datetime'

Knockdown of AKT3 Activates HER2 and DDR Kinases in Bone-Seeking Breast Cancer Cells, Promotes Metastasis In Vivo and Attenuates the TGFβ/CTGF Axis

Bone metastases frequently occur in breast cancer patients and lack appropriate treatment options. Hence, understanding the molecular mechanisms involved in the multistep process of breast cancer bone metastasis and tumor-induced osteolysis is of paramount interest. The serine/threonine kinase AKT plays a crucial role in breast cancer bone metastasis but the effect of individual AKT isoforms remains unclear. Therefore, AKT isoform-specific knockdowns were generated on the bone-seeking MDA-MB-231 BO subline and the effect on proliferation, migration, invasion, and chemotaxis was analyzed by live-cell imaging. Kinome profiling and Western blot analysis of the TGFβ/CTGF axis were conducted and metastasis was evaluated by intracardiac inoculation of tumor cells into NOD scid gamma (NSG) mice. MDA-MB-231 BO cells exhibited an elevated AKT3 kinase activity in vitro and responded to combined treatment with AKT- and mTOR-inhibitors. Knockdown of AKT3 significantly increased migration, invasion, and chemotaxis in vitro and metastasis to bone but did not significantly enhance osteolysis. Furthermore, knockdown of AKT3 increased the activity and phosphorylation of pro-metastatic HER2 and DDR1/2 but lowered protein levels of CTGF after TGFβ-stimulation, an axis involved in tumor-induced osteolysis. We demonstrated that AKT3 plays a crucial role in bone-seeking breast cancer cells by promoting metastatic potential without facilitating tumor-induced osteolysis

Lipocalin-2 induces NLRP3 inflammasome activation via HMGB1 induced TLR4 signaling in heart tissue of mice under pressure overload challenge

Lipocalin-2 (also known as NGAL) levels are elevated in obesity and diabetes yet relatively little is known regarding effects on the heart. We induced pressure overload (PO) in mice and found that lipocalin-2 knockout (LKO) mice exhibited less PO-induced autophagy and NLRP3 inflammasome activation than Wt. PO-induced mitochondrial damage was reduced and autophagic flux greater in LKO mice, which correlated with less cardiac dysfunction. All of these observations were negated upon adenoviral-mediated restoration of normal lipocalin-2 levels in LKO. Studies in primary cardiac fibroblasts indicated that lipocalin-2 enhanced priming and activation of NLRP3-inflammasome, detected by increased IL-1β, IL-18 and Caspase-1 activation. This was attenuated in cells isolated from NLRP3-deficient mice or upon pharmacological inhibition of NLRP3. Furthermore, lipocalin-2 induced release of HMGB1 from cells and NLRP3-inflammasome activation was attenuated by TLR4 inhibition. We also found evidence of increased inflammasome activation and reduced autophagy in cardiac biopsy samples from heart failure patients. Overall, this study provides new mechanistic insight on the detrimental role of lipocalin-2 in the development of cardiac dysfunction