Alterations along the Hypothalamic-Pituitary-Thyroid Axis of the Zebrafish ( Danio rerio

In the past, various approaches have been developed to detect adverse effects of pollutants on the thyroid of vertebrates, most of these with special emphasis on the South African clawed frog, Xenopus laevis. Although fish are primarily affected by thyroid-disrupting chemicals, studies into alterations of the thyroid of fish are scarce. Therefore, effects of the reference compound propylthiouracil on histopathology of the thyroid axis were analyzed in a modified early life-stage test with zebrafish (Danio rerio) exposed to propylthiouracil. The test substance induced dose-dependent alterations of thyroidal tissue concomitant with increases in the number of surrounding blood vessels. Despite this massive proliferation of the thyroid, zebrafish were not able to maintain thyroxin concentrations. The pituitary was affected displaying significant alterations in thyroid-stimulating hormone cell counts. Quantitative evaluation of pituitary surface areas revealed a dose-dependent increase of adenohypophyseal tissue. Distinct histopathological effects may contribute to a more easy identification and interpretation of alterations induced by thyroid-disrupting chemicals

Specificity of time- and dose-dependent morphological endpoints in the fish embryo acute toxicity (FET) test for substances with diverse modes of action: : the search for a “fingerprint”

Acknowledgements This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No 681002 (GA, art. 38.1.2). The raw data for valproic acid, acrylamide, and colchicine were provided by Katharina Brotzmann, Annika Batel, and Ann-Kathrin Lörracher, respectively. Range-finding experiments were previously also conducted by Susanna Mieck, Dr. Lisa Hanslik, and Dr. Florian Zindler. Thanks are due to Jonathan Griffiths and Christoph Gade for carefully editing parts of the manuscript. Funding Open Access funding enabled and organized by Projekt DEAL. This project has received funding from the European Union’s Horizon 2020 research and innovation program under the grand agreement No 681002.Publisher PD

Получение желаемых передаточных функций импульсных систем автоматического управления

Рассмотрена задача получения желаемых передаточных функций импульсных систем автоматического управления. Предложен способ синтеза передаточных функций низкого порядка по прямым показателям качества, обеспечивающий конечное время переходного процесса

The Addressee Types of the Internet Open Letters

The author considers open letters on Internet sites as a variation of an epistolary genre. A novel type of an open letter, a letter to the future is described. Computer mediated communication as a form of public discourse increases the number of recipients of the letters, introducing changes to the conventional model of a letter addressee. It is concluded that messages on the open letters sites, high in personalization (i.e. private) and low in exclusivity (i.e. public), allow bridging the gap between mass and personal communication, thus remediating an epistolary genre

Разработка информационной системы контроля и учета посещаемости спортивного учреждения

Работа направлена на разработку информационной системы для контроля и учета посещаемости клиентов спортивного учреждения на основе NFC / RFID датчиков. Приведен анализ существующих решений и обоснование выбора используемых технологий.The work is aimed at developing an information system for monitoring and recording attendance of clients of a sports establishment based on NFC / RFID sensors. The analysis of existing solutions and justification of the choice of the technologies used are given

The sensitivity of the zebrafish embryo coiling assay for the detection of neurotoxicity by compounds with diverse modes of action

Open Access via the Springer Agreement Funding: This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 681002 (EU-ToxRisk).Peer reviewedPublisher PD

Сегментация изображения с помощью программного средства MatLab

In computer vision, image segmentation is the process of partitioning a digital image intomultiple segments (sets of pixels, also known as superpixels). The goal of segmentation is to simplify and/orchange the representation of an image into something that is more meaningful and easier to analyze. Imagesegmentation is typically used to locate objects and boundaries (lines, curves, etc.) in images. More precisely,image segmentation is the process of assigning a label to every pixel in an image such that pixels with thesame label share certain characteristics. The result of image segmentation is a set of segments that collectivelycover the entire image, or a set of contours extracted from the image

РОЗВИТОК СИСТЕМИ ПОЧАТКОВОЇ ТА СЕРЕДНЬОЇ ОСВІТИ У МЕНОНІТСЬКИХ КОЛОНІЯХ КАТЕРИНОСЛАВСЬКОГО ПОВІТУ НАПРИКІНЦІ XIX – НА ПОЧАТКУ XX СТ.

Проблеми формування системи початкової освіти постійно знаходи-лись у фокусі громадської уваги менонітського соціуму, який намагався шля-хом надзвичайної концентрації інтелектуальних, фінансових та господарчих зусиль сприяти її всебічному становленню та розвитку. Ґенеза менонітсько-го віровчення, темпи та масштаб його поширення серед населення північ-них регіонів Західної Європи були об'єктивно зумовлені наявністю у пред-ставників даного релігійного руху елементарних освітянських навичок (вміння читати та писати), які були необхідні для раціонального тлумачен-ня біблійних та релігійних текстів. Поява розгалуженої освітянської системи в громадах менонітів була детермінована специфікою віровчення спільноти, її нагальними релігійними, церковними та громадськими потребами. Зазначимо, що меноніти розглядали шкільництво як один із засобів набуття «освічено-го благочестя»

Occurrence and distribution of resistance to QoI fungicides in populations of Podosphaera fusca in south central Spain

Cucurbit powdery mildew caused by Podosphaera fusca limits crop production in Spain. Since its management is strongly dependent on chemicals, the rational design of control programmes requires a good understanding of the fungicide resistance phenomenon in field populations. Fifty single-spore isolates of P. fusca were tested for sensitivity to three quinone-outside inhibiting (QoI) fungicides: azoxystrobin, kresoxim-methyl and trifloxystrobin. Minimum inhibitory concentration (MIC) values for QoI-sensitive isolates were found to range from 0.25 to 10 μg ml−1 for azoxystrobin to 5–25 μg ml−1 for kresoxim-methyl, using a leaf disc-based bioassay. High levels of cross-resistance to QoI fungicides were found. Eleven isolates showed resistance to the three QoI fungicides tested with MIC and EC50 values >500 μg ml−1 resulting in RF values as high as >715 and >1000 for trifloxystrobin and azoxystrobin, respectively. A survey of P. fusca QoI resistance was carried out in different provinces located in the south central area of Spain during the cucurbit growing seasons in 2002, 2003 and 2004. Examination of a collection of 250 isolates for QoI resistance revealed that 32% were resistant to the three fungicides tested; the provinces of Ciudad Real, Córdoba and Murcia being the locations with the highest frequencies of resistance (44–74%). By contrast, no resistance was found in Badajoz, and relatively low frequencies were observed in Almería and Valencia (10–13%). Nearly 50% of resistant isolates were collected from melon plants. Based on these data, recommendations about the use of QoI fungicides for cucurbit powdery mildew management in the sampled areas are made.Estación Experimental “La Mayora” (CSIC), Algarrobo-Costa, E-29750 Málaga, Spain Grupo de Microbiología y Patología Vegetal-Unidad Asociada a CSIC, Departamento de Microbiología, Facultad de Ciencias, Universidad de Málaga, E-29071 Málaga, SpainPeer reviewe