2,057 research outputs found
Cloning and expression of hmgr1 gene from Hevea brasiliensis
Biosynthesis of natural rubber (cis-1,4-polyisoprene) takes place through mevalonate pathway in Hevea. The enzyme 3-hydroxy- 3-methyl glutaryl-CoA reductase (HMGR), which catalyses the synthesis of mevalonate from HMG-CoA is a key regulatory enzyme in this pathway. This study aimed to clone and express hmgr1 gene, in order to obtain the HMGR protein in vitro and to further use this protein as a marker for yield potential in Hevea. For this purpose, mRNA was isolated from the latex of Hevea (clone RRII 105). cDNA was synthesized and PCR amplification of coding region of hmgr1 was performed using hmgr1 specific primers. The PCR amplified product (~1.8 kb) was cloned into an expression vector (pRSET-A) and transformed into E. coli (BL21DE3) cells. Protein expression in transformed cells when monitored by SDS-PAGE analysis indicated the presence of HMGR protein (61.6 kDa). The protein would be used for developing specific antibody that could be further utilized for the quantification of HMGR in different Hevea clones for screening the yield potential. The details of cloning and expression of hmgr1 are presented and discussed
Role of microenvironment in the mixed Langmuir-Blodgett films
This paper reports the pi-A isotherms and spectroscopic characteristics of
mixed Langmuir and Langmuir-Blodgett (LB) films of non-amphiphilic carbazole
(CA) molecules mixed with polymethyl methacrylate (PMMA) and stearic acid (SA).
pi-A isotherm studies of mixed monolayer and as well as also the collapse
pressure study of isotherms definitely conclude that CA is incorporated into
PMMA and SA matrices. However CA is stacked in the PMMA/SA chains and forms
microcrystalline aggregates as is evidenced from the scanning electron
micrograph picture. Nature of these aggregated species in the mixed LB films
has been revealed by UV-Vis absorption and fluorescence spectroscopic studies.
The presence of two different kinds of band systems in the fluorescence spectra
of the mixed LB films have been observed. This may be due to the formation of
low dimensional aggregates in the mixed LB films. Intensity distribution of
different band system is highly sensitive to the microenvironment of two
different matrices as well as also on the film thicknessComment: 11 pages, 5 figure
Mersenne Primes, Polygonal Anomalies and String Theory Classification
It is pointed out that the Mersenne primes and associated
perfect numbers play a significant role in string
theory; this observation may suggest a classification of consistent string
theories.Comment: 10 pages LaTe
Gyrations: The Missing Link Between Classical Mechanics with its Underlying Euclidean Geometry and Relativistic Mechanics with its Underlying Hyperbolic Geometry
Being neither commutative nor associative, Einstein velocity addition of
relativistically admissible velocities gives rise to gyrations. Gyrations, in
turn, measure the extent to which Einstein addition deviates from commutativity
and from associativity. Gyrations are geometric automorphisms abstracted from
the relativistic mechanical effect known as Thomas precession
Non-Perturbative Corrections and Modularity in N=1 Type IIB Compactifications
Non-perturbative corrections and modular properties of four-dimensional type
IIB Calabi-Yau orientifolds are discussed. It is shown that certain
non-perturbative alpha' corrections survive in the large volume limit of the
orientifold and periodically correct the Kahler potential. These corrections
depend on the NS-NS two form and have to be completed by D-instanton
contributions to transform covariantely under symmetries of the type IIB
orientifold background. It is shown that generically also the D-instanton
superpotential depends on the two-form moduli as well as on the complex
dilaton. These contributions can arise through theta-functions with the dilaton
as modular parameter. An orientifold of the Enriques Calabi-Yau allows to
illustrate these general considerations. It is shown that this compactification
leads to a controlled four-dimensional N=1 effective theory due to the absence
of various quantum corrections. Making contact to the underlying topological
string theory the D-instanton superpotential is proposed to be related to a
specific modular form counting D3, D1, D(-1) degeneracies on the Enriques
Calabi-Yau.Comment: 35 page
Micro-Hall Magnetometry Studies of Thermally Assisted and Pure Quantum Tunneling in Single Molecule Magnet Mn12-Acetate
We have studied the crossover between thermally assisted and pure quantum
tunneling in single crystals of high spin (S=10) uniaxial single molecule
magnet Mn12-acetate using micro-Hall effect magnetometry. Magnetic hysteresis
experiments have been used toinvestigate the energy levels that determine the
magnetization reversal as a function of magnetic field and temperature. These
experiments demonstrate that the crossover occurs in a narrow (~0.1 K) or broad
(~1 K) temperature interval depending on the magnitude and direction of the
applied field. For low external fields applied parallel to the easy axis, the
energy levels that dominate the tunneling shift abruptly with temperature. In
the presence of a transverse field and/or large longitudinal field these energy
levels change with temperature more gradually. A comparison of our experimental
results with model calculations of this crossover suggest that there are
additional mechanisms that enhance the tunneling rate of low lying energy
levels and broaden the crossover for small transverse fields.Comment: 5 pages, 5 figure
Earthworm management in tropical agroecosystems
Collaborative research in the Macrofauna project has enabled development of some techniques that presently are at different stages of advancement, from promising pilot experiments (tomato production and inoculation in plant nursery bags at Yurimaguas and in India) to the fully developed technique of massive worm production and biofertilization of tea gardens in Tamil Nadu (India) (patent deposited). Failures have also helped to gain better insight into the potential feasibility of techniques that had been considered in the objectives of this project. Endogeic earthworms (#Pontoscolex corethrurus$) may be produced in large quantities, i.e.about 12000 worms (1.6-2.8 kg live wt)/m2/year in specific culture beds using either sawdust (Yurimaguas, Peru) or a mixture of high and low quality materials (Tamil Nadu, India) mixed into soil as substrates. Cost of production of 1 kg of earthworm biomass through bed culture is about 3.6 Euro, much lower than the cost of hand collection of worms from pastures/grasslands where these species are abundant (6-125 Euro depending on the cost of labour and earthworm density). The theorical value of an active earthworm community with an average biomass of 400 kg live wt has been estimated at 1400 Euro, the price that it would cost to reintroduce an equivalent biomass produced in our culture units, indicating the cost of land restoration. Direct inoculation of earthworms in the field to improve production may only affect plant growth positively if a large biomass (greater than 30 g live wt/m2) is inoculated from the beginning. An alternative may be to concentrate the inoculum in small areas regularly distributed across the field... (D'après résumé d'auteur
Negative Energy Density in Calabi-Yau Compactifications
We show that a large class of supersymmetric compactifications, including all
simply connected Calabi-Yau and G_2 manifolds, have classical configurations
with negative energy density as seen from four dimensions. In fact, the energy
density can be arbitrarily negative -- it is unbounded from below.
Nevertheless, positive energy theorems show that the total ADM energy remains
positive. Physical consequences of the negative energy density include new
thermal instabilities, and possible violations of cosmic censorship.Comment: 25 pages, v2: few clarifying comments and reference adde
Open String Attractors
We present a simple example of a supersymmetric attractor mechanism in the
purely open string context of D-branes embedded in curved space-time. Our
example involves a class of D3-branes embedded in the 2-charge D1-D5 background
of type IIB whose worldvolume contains a 2-sphere. Turning on worldvolume
fluxes, these branes carry induced (p,q) string charges. Supersymmetric
configurations display a flow of the open string moduli towards an attractor
solution independent of their asymptotics. The equations governing this
mechanism closely resemble the attractor flow equations for supersymmetric
black holes in closed string theory. The BPS equations take the form of a
gradient flow and describe worldvolume solitons interpolating between an AdS_2
geometry where the two-sphere has collapsed, and an attractor solution with
AdS_2 x S^2 geometry. In these limiting solutions, the preserved supersymmetry
is enhanced from 4 to 8 supercharges. We also discuss the interpretation of our
solutions as intersecting brane configurations placed in the D1-D5 background,
as well as the S-duality transformation to the F1-NS5 background.Comment: 37 pages, 6 figures. v2: small corrections, figure and references
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