Brussels, a sustainable city

Observations As a reminder: Sustainable defines that which meets the current generations’ needs without jeopardising the possibilities for future generations to meet their own needs (that is to say, without exceeding resource renewal rates). More simply put, that which is capable of continuing is sustainable. A layout inherited from the modernist era The zoning of the city’s territory Brussels has been marked by monofunctionalism over the past few decades. This applies to both its central nei..

Brussel, duurzame stad

Vaststellingen Duurzame ontwikkeling is een ontwikkeling waarbij de huidige wereldbevolking in haar behoeften voorziet zonder de komende generaties te beperken om in hun behoeften te voorzien (namelijk zonder het vermogen om de rijkdommen te vernieuwen aan te tasten). Eenvoudiger gesteld: duurzaam is wat kan blijven bestaan… Stedenbouwkunde: een erfenis uit het modernistische tijdperk De indeling van het Brussels grondgebied De laatste decennia werd Brussel gekenmerkt door monofunctionalisme,..

Long-Peptide Cross-Presentation by Human Dendritic Cells Occurs in Vacuoles by Peptide Exchange on Nascent MHC Class I Molecules.

Cross-presentation enables dendritic cells to present on their MHC class I molecules antigenic peptides derived from exogenous material, through a mechanism that remains partly unclear. It is particularly efficient with long peptides, which are used in cancer vaccines. We studied the mechanism of long-peptide cross-presentation using human dendritic cells and specific CTL clones against melanoma Ags gp100 and Melan-A/MART1. We found that cross-presentation of those long peptides does not depend on the proteasome or the transporter associated with Ag processing, and therefore follows a vacuolar pathway. We also observed that it makes use of newly synthesized MHC class I molecules, through peptide exchange in vesicles distinct from the endoplasmic reticulum and classical secretory pathway, in an SEC22b- and CD74-independent manner. Our results indicate a nonclassical secretion pathway followed by nascent HLA-I molecules that are used for cross-presentation of those long melanoma peptides in the vacuolar pathway. Our results may have implications for the development of vaccines based on long peptides