Research Notes: Linkage tests between Sp1 and Ti seed proteins

Polyacrylamide gel electrophoresis has been used to study the Ti and Sp1 seed proteins of the soybean. The Ti protein has been identified as the Kunitz soybean trypsin inhibitor or SBTI-A2 (Kunitz, 1945; Rackis et al., 1962; Singh, Wilson and Hadley, 1969). The three fonns of SBTI-A2 designated as Ti1, Ti2 and Ti3 are electrophoretically distinguishable from one another by their different Rf values of 0.79, 0.75 and 0.83 (Rf= mobility relative to the dye front in a 10% polyacrylamide gel anodic system using a pH 8.3 Tris-glycine buffer) respectively

Research Notes: The Gene Symbols Sp1a and Sp1b Assigned to Larsen and Caldwell\u27s Seed Protein Bands A and B

Larson (1967), using acrylamide gel electrophoresis, described two seed proteins in soybean seed and noted they were variety specific. The inheritance of these proteins (although the proteins were not characterized) was reported as being controlled by two codominant alleles at a single locus (Larsen and Caldwell, 1968); gene symbols were not assigned. The letters A and \u27\u27B\u27\u27 were used to designate the two different seed protein bands

Studies on Guar - Cyamopsis Tetragonoloba (L.) Taub.

Plant Breeding and Genetic

GUAR: Seed, Plant and Population Studies

The Oklahoma Agricultural Experiment Station periodically issues revisions to its publications. The most current edition is made available. For access to an earlier edition, if available for this title, please contact the Oklahoma State University Library Archives by email at [email protected] or by phone at 405-744-6311

Calcium oxalate crystal macropatterns in leaves of species from groups Glycine and Shuteria (Glycininae; Phaseoleae; Papilionoideae; Fabaceae)

Calcium oxalate crystal macropatterns in leaves were characterized for 69 species (and two Glycine tomentella cytotypes) from 14 of 16 genera in two legume groups, Glycine and Shuteria, to determine whether they share a common macropattern. A leaf clearing method was used to visualize the crystals. All 69 species (and two Glycine tomentella cytotypes) displayed prismatic crystals associated with leaf veins and vein endings. In contrast, mesophyll crystals occurred in 76.8% of 69 species and two G. tomentella cytotypes, and varied from a few to many. Conversely, only 40.9% of 22 Glycine species (in group Glycine) lacked mesophyll crystals, while 8.7% of 23 species of six genera associated with Glycine (in group Glycine) lacked mesophyll crystals. Thus 24.4% of 45 species of seven combined genera in group Glycine lacked mesophyll crystals. With seven genera in group Shuteria, 20.8% of 24 species lacked mesophyll crystals. The consistently present vein crystals varied in size and shape, so their length–width (Stubby versus Long) crystal ratios were determined for primary, secondary, and tertiary veins, and vein endings. Two trends were evident: Long-crystal ratios increased from primary veins to vein endings in species in both groups, and the perennial and annual Glycine species showed this condition to a greater extent than all the non-Glycine species. In some cases, taxonomically closely associated species were quite similar in their macropattern and presence or absence of mesophyll crystals. These results should be of value to future studies dealing with taxonomy and phylogeny of species in these two leguminous groups