Commissioning RHICS electron lens

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A NADPH cytochrome P450 reductase competent mutagenicity tester strain for the expression of human cytochrome P450: Comparison of three types of expression systems.

Currently three different methods have been taken to develop new mutagenicity tester strains containing human cytochrome P450s (CYPs). Each of these use a single expression vector. In this paper we describe a fourth approach, i.e., the coexpression of a CYP and its electron-transfer flavoprotein, NADPH CYP reductase (RED), encoded by two different expression vectors. The Escherichia coli mutagenicity tester strain BMX100 has been expanded to a strain, MTC which stably expresses human RED. This new tester strain permits the biplasmid coexpression of human CYP1A2 and RED (MTC1A2). This novel strain can be used for the determination of the mutagenicity of chemicals known to be procarcinogens and metabolized by CYP1A2. The mutagenicity tester strain MTC1A2 was compared with: (i) BMX100 using the post-mitochondrial rat liver fraction (S9); (ii) BMX100 with expressing CYP1A2 alone (iii) or with expressing CYP1A2 fused to rat RED or (iv) with expressing CYP1A2, bicistronically coexpressed with rat RED. The biplasmid RED/CYP coexpression system generated a strain with the highest methoxy- and ethoxy-resorufin dealkylase activities and highest mutagenic activities for the procarcinogens 2-aminoanthracene (2AA), aflatoxin B1 (AFB1) and 2-amino-3-methylimidazo(4,5-f)quinoline (IQ). Furthermore, the metabolism of 2AA and IQ was detected more efficiently using the MTC1A2 strain than with the BMX100 strain plus the standard rodent liver S9 metabolic system

Unfavoured signature partner superdeformed bands associated with proton excitations in 151^{151}Tb

The author first notes that Google, the trade mark, the project, the utilization - the "googling"- are social facts proven by the numbers – number of net surfers, of requests, of uses- and by the signs of adhesion - linguistics, economic, social. A socio linguistic analysis of the speeches of the persons in charge of Google and of users indicate that the social fact "googling" results in the emergence of a culture and a world community which shares it. They are supported by the language and also by the myths that were created and largely maintained by the owners the mark "Google Inc". The conclusion is that the current organization of the market of services on the Internet makes that Google Inc. is almost the only institution to know the population of the googlers. In order not to be subjected to this monopoly, however comfortable it is, the author proposes to develop a research program on the uses and users of Google