Effect of seminal plasma removal, washing solutions, and centrifugation regimes on boer goat semen cryopreservation

Three experiments were carried out to improve semen quality during cryopreservation process. Total motility, forward motility, acrosome integrity, live spermatozoa, and normal spermatozoa were measured as semen quality. In Experiment 1, the effects of seminal plasma removal were analyzed by using two different extenders (GE and FE). The removal of seminal plasma gave higher and significant (P<0.05) effect in the total motility, forward motility, and live spermatozoa after cryopreservation. For two different extenders, however, the differences were not observed on the semen quality. In Experiment 2, three different washing solutions (namely, phosphate buffered saline, normal saline and Tris-based extender) were tested to evaluate the effects of semen quality after cryopreservation. Tris-based extender (TCG) conferred the highest (P<.05) sperm quality values in the total motility, forward motility, and live spermatozoa after cryopreservation. In Experiment 3, the effects of different centrifugation regimes (3000 × g for 3 min, 1600 × g for 10 min, 800 × g for 15 min) were evaluated on Boer semen quality. Semen quality parameters (namely, total motility, forward motility, acrosome integrity, and live spermatozoa) were significantly (P<.05) higher for cryopreserved spermatozoa centrifuged with 3000 × g for 3 min than the others. In conclusion, the removal of seminal plasma, washing solution TCG, and the use short-term centrifugation with a relative high g-force could contribute to the increased Boer semen quality after cryopreservation

Factors associated with psychological distress among Myanmar residents during COVID-19 pandemic crises

Background: COVID-19 pandemic reached a public health emergency status of international concern. The impacts and events associated with this were associated with adverse psychological impacts among the general public globally. This study aimed to determine the prevalence of psychological distress and to identify predictors associated with psychological distress due to the COVID-19 pandemic among the population in Myanmar. Design and Methods: A cross-sectional survey was conducted from March to April 2020 among adults, 18 years old and above, who reside in Myanmar through a structured questionnaire distributed in social media platforms. Univariate and Bivariate analyses were used to estimate the prevalence of COVID-19 Peritraumatic Distress Index (CPDI) symptoms and to test the associations between CPDI and the exposure variables. Logistic Regression Analysis was done to identify significant predictors of distress. Results: There were 530 participants in this study.37.4% of them did not have psychological distress,55.6% experienced mild to moderate psychological distress, and 7% experienced severe psychological distress due to COVID-19 pandemic. Simple and Multiple Logistic Regression Analyses were performed to determine the factors associated with psychological distress due to COVID-19. Conclusions: It was shown that the self-employed group and age group older than 45 years old had more psychological distress than others. However, Buddhists and people from the capital city had less distress than other religions and districts. This study recommends the government to develop better strategies for self-employed groups, elders, and the poor for a support, relief, and resettlement of their ruined status

Effect of sugars on characteristics of Boer goat semen after cryopreservation

In order to improve Boer goat semen quality during cryopreservation process, the influence of sugar supplementation on semen characteristics of sperm were investigated. Three experiments were carried out to investigate the effect of (a) addition of two monosaccharides (fructose and glucose) and two disaccharides sugars (trehalose and sucrose) (b) sugar combination (fructose and trehalose, sucrose and trehalose, glucose and trehalose), and control (glucose without trehalose) (c) different concentrations of trehalose on cryopreservation using Tris based extender. The total motility, forward motility, viability, normal spermatozoa, acrosome integrity and membrane integrity were assessed subjectively. Differences were not detected among monosaccharides, but glucose increased (P 0.05) among disaccharide sugar supplementation. Combination of glucose and trehalose significantly improved the characteristics of Boer spermatozoa after cryopreservation (P < 0.05). Supplementation of trehalose (198.24 mM) into the glucose extender significantly increased total motility, forward motility, live spermatozoa, acrosome integrity and membrane integrity following cryopreservation (P < 0.05). In conclusion, glucose had the better ability to support Boer sperm motility and movement patterns. Combination of monosaccharide (glucose) and disaccharide (trehalose) improved semen quality following cryopreservation. Trehalose supplementation at the concentration of 198.24 mM to the glucose extender conferred the greater improvement of semen quality for Boer semen cryopreservation

Confirmation of Skywalker Hoolock Gibbon (Hoolock tianxing) in Myanmar extends known geographic range of an endangered primate

Characterizing genetically distinct populations of primates is important for protecting biodiversity and effectively allocating conservation resources. Skywalker gibbons (Hoolock tianxing) were first described in 2017, with the only confirmed population consisting of 150 individuals in Mt. Gaoligong, Yunnan Province, China. Based on river geography, the distribution of the skywalker gibbon has been hypothesized to extend into Myanmar between the N’Mai Kha and Ayeyarwaddy Rivers to the west, and the Salween River (named the Thanlwin River in Myanmar and Nujiang River in China) to the east. We conducted acoustic point-count sampling surveys, collected noninvasive samples for molecular mitochondrial cytochrome b gene identification, and took photographs for morphological identification at six sites in Kachin State and three sites in Shan State to determine the presence of skywalker gibbons in predicted suitable forest areas in Myanmar. We also conducted 50 semistructured interviews with members of communities surrounding gibbon range forests to understand potential threats. In Kachin State, we audio-recorded 23 gibbon groups with group densities ranging between 0.57 and 3.6 group/km2. In Shan State, we audio-recorded 21 gibbon groups with group densities ranging between 0.134 and 1.0 group/km2. Based on genetic data obtained from skin and saliva samples, the gibbons were identified as skywalker gibbons (99.54–100% identity). Although these findings increase the species’ known population size and confirmed distribution, skywalker gibbons in Myanmar are threatened by local habitat loss, degradation, and fragmentation. Most of the skywalker gibbon population in Myanmar exists outside protected areas. Therefore, the IUCN Red List status of the skywalker gibbon should remain as Endangered

Political transition and emergent forest-conservation issues in Myanmar.

Political and economic transitions have had substantial impacts on forest conservation. Where transitions are underway or anticipated, historical precedent and methods for systematically assessing future trends should be used to anticipate likely threats to forest conservation and design appropriate and prescient policy measures to counteract them. Myanmar is transitioning from an authoritarian, centralized state with a highly regulated economy to a more decentralized and economically liberal democracy and is working to end a long-running civil war. With these transitions in mind, we used a horizon-scanning approach to assess the 40 emerging issues most affecting Myanmar's forests, including internal conflict, land-tenure insecurity, large-scale agricultural development, demise of state timber enterprises, shortfalls in government revenue and capacity, and opening of new deforestation frontiers with new roads, mines, and hydroelectric dams. Averting these threats will require, for example, overhauling governance models, building capacity, improving infrastructure- and energy-project planning, and reforming land-tenure and environmental-protection laws. Although challenges to conservation in Myanmar are daunting, the political transition offers an opportunity for conservationists and researchers to help shape a future that enhances Myanmar's social, economic, and environmental potential while learning and applying lessons from other countries. Our approach and results are relevant to other countries undergoing similar transitions

Political transition and emergent forest-conservation issues in Myanmar.

Political and economic transitions have had substantial impacts on forest conservation. Where transitions are underway or anticipated, historical precedent and methods for systematically assessing future trends should be used to anticipate likely threats to forest conservation and design appropriate and prescient policy measures to counteract them. Myanmar is transitioning from an authoritarian, centralized state with a highly regulated economy to a more decentralized and economically liberal democracy and is working to end a long-running civil war. With these transitions in mind, we used a horizon-scanning approach to assess the 40 emerging issues most affecting Myanmar's forests, including internal conflict, land-tenure insecurity, large-scale agricultural development, demise of state timber enterprises, shortfalls in government revenue and capacity, and opening of new deforestation frontiers with new roads, mines, and hydroelectric dams. Averting these threats will require, for example, overhauling governance models, building capacity, improving infrastructure- and energy-project planning, and reforming land-tenure and environmental-protection laws. Although challenges to conservation in Myanmar are daunting, the political transition offers an opportunity for conservationists and researchers to help shape a future that enhances Myanmar's social, economic, and environmental potential while learning and applying lessons from other countries. Our approach and results are relevant to other countries undergoing similar transitions

Socializing One Health: an innovative strategy to investigate social and behavioral risks of emerging viral threats

In an effort to strengthen global capacity to prevent, detect, and control infectious diseases in animals and people, the United States Agency for International Development’s (USAID) Emerging Pandemic Threats (EPT) PREDICT project funded development of regional, national, and local One Health capacities for early disease detection, rapid response, disease control, and risk reduction. From the outset, the EPT approach was inclusive of social science research methods designed to understand the contexts and behaviors of communities living and working at human-animal-environment interfaces considered high-risk for virus emergence. Using qualitative and quantitative approaches, PREDICT behavioral research aimed to identify and assess a range of socio-cultural behaviors that could be influential in zoonotic disease emergence, amplification, and transmission. This broad approach to behavioral risk characterization enabled us to identify and characterize human activities that could be linked to the transmission dynamics of new and emerging viruses. This paper provides a discussion of implementation of a social science approach within a zoonotic surveillance framework. We conducted in-depth ethnographic interviews and focus groups to better understand the individual- and community-level knowledge, attitudes, and practices that potentially put participants at risk for zoonotic disease transmission from the animals they live and work with, across 6 interface domains. When we asked highly-exposed individuals (ie. bushmeat hunters, wildlife or guano farmers) about the risk they perceived in their occupational activities, most did not perceive it to be risky, whether because it was normalized by years (or generations) of doing such an activity, or due to lack of information about potential risks. Integrating the social sciences allows investigations of the specific human activities that are hypothesized to drive disease emergence, amplification, and transmission, in order to better substantiate behavioral disease drivers, along with the social dimensions of infection and transmission dynamics. Understanding these dynamics is critical to achieving health security--the protection from threats to health-- which requires investments in both collective and individual health security. Involving behavioral sciences into zoonotic disease surveillance allowed us to push toward fuller community integration and engagement and toward dialogue and implementation of recommendations for disease prevention and improved health security

Risk Level Prediction for Heart Disease using Decision Tree Induction

Heart Disease was the major cause of causalitiesin most of the countries. According to the medicalrecords, heart disease kills one person in very sorttime. Classification and prediction are the forms ofdata analysis that can be used to extract models forimportant classes or to predict future data trends. Inthis paper, decision tree induction algorithm is usedto classify the risk level for heart disease. Decisiontree is a flow-chart-like tree structure, where eachinternal node denotes a test on an attributes, eachbranch represents an outcome of the test, and theleaf nodes represent classes or class distributions.This system generates the understandable rules foruser and estimates the accuracy for classifier.Depending on the attribute values of the data set,this system can classify the risk level of heartdisease whether it is in serious or normal conditionsfor patients. Thus, the user can test his or hermedical check concerned with their heart.Moreover, the system can provide the classifieraccuracy by using Holdout Method

Morphological and Anatomical Characteristics of Camellia sinensis (L.) Kuntze from Two Different Localities

Morphological and anatomical characteristics of Camellia sinensis (L.) Kuntze belonging to family Theaceae were studied at Department of Botany, University of Mandalay. The specimens were collected from Naung Cho Township of Northern Shan State and Mogok Township of Mandalay Region from June to October, 2017. The morphological and anatomical characteristics of leaves, stems and roots were studied, described, discussed and their photographs and photomicrographs were also presented. In morphological characteristics, the sizes of leaves, petioles and the numbers of petals were showed variable from one locality to another. The anatomical characteristics showed the differences in thickness of cell layers, the numbers and sizes of vascular bundles of leaves, stems and roots. The sclereids were observed in the ground tissues of petioles and midribs. The vascular bundles of stems were collateral type and roots were radial type and polyarch. The morphological and anatomical characteristics are useful in species confirmation and certain identification

Anatomical Characteristics of Camelliasinensis (L.)Kuntze fromThree Localities in Myanmar

Anatomical characteristics of Camellia sinensis(L.)Kuntze belonging to the family Thaeceae were studied. Camellia sinensis(L.)Kuntzeis known as 'laphet or tea' in Myanmar.The plant specimens were collected from three localities of Pinlaung, Panglon and Ywangan Townships of Southern Shan State in Myanmar in 2018. The collected specimens were identified at the Department of Botany, University of Mandalay. In the anatomical studies, the small pieces of specimens were cut into thin sections by using a rotary microtome according to Johansen (1940). In this research, the plants were perennial shrubs to small trees. The leaves were simple, alternate and exstipulate. In the anatomical characteristics, the numbers and sizes of cells and thickness of cell layers were slightly different from one locality to another. The stomata were anomocytic type. The sclereids were observed in the petioles, laminae and midribs. The vascular bundles were collateral type in stems and radial type in root. The vascular bundles of roots were polyarch. Pith was found in roots of three localities. The similarities and differences of anatomical characteristics of these species from different localities in Myanmar would provide for the useful identification of the genus,Camellia