Nuclear magnetic resonance monitoring of centrifugal partition chromatography in pH-zone-refining mode

International audienceThe coupling of centrifugal partition chromatography to NMR spectroscopy as detection and analysis method is investigated. Separation of a test mixture of three N-2,4-dinitrophenyl amino acids was performed in pH-zone-refining elution mode. The NMR and chromatography instrumentation was very similar to the one of the LC–NMR technique

Isolation of indole alkaloids from Catharanthus roseus by centrifugal partition chromatography in the pH-zone refining mode

International audienceCentrifugal partition chromatography (CPC) in the pH-zone refining mode allowed a preparative and efficient isolation of vindoline, vindolinine, catharanthine and vincaleukoblastine from a crude mixture of Catharanthus roseus alkaloids. The separation protocol was tested with a synthetic mixture of vindoline, catharanthine and vincaleukoblastine. The fraction content was analyzed and the results compared with theoretical chromatograms obtained by numerical simulation. The increase in injected sample mass results in an improvement of the purity of the isolated compounds. This observation, confirmed by theory, is of prime importance for the development of preparative pH-zone refining CPC as a preparative separation method

Moandaensine, a dimeric indole alkaloid from Strychnos moandaensis (Loganiaceae)

Moandaensine contains a rare anhydronium base subunit. It presents a moderate antiplasmodial activity with IC values of 11.2 microM and 9.2 microM against , respectively, the chloroquino sensitive FCA 20 GHA and chloroquino resistant W2 strains of Plasmodium falciparu