Measuring Serum Toxicity Markers to Evaluate the Safety of Commercially Available Spirulina Products in Mice

This research article published by Current Research in Nutrition and Food Science, 2021Spirulina a multicellular, blue-green alga has drawn attention as a viable food supplement due to its suitable nutrient composition, however, there is a dearth of information regarding its safety. This study aimed to measure the heavy metal concentrations in commercially available Spirulina products and evaluate the safety using the in vivo method. A total of 54 mice were randomly divided into three groups; Group 1 (n = 18) served as a control and received a basal diet. Group 2 (n = 20) served as a test and received Spirulina powder (15%) blended with a basal diet. Group 3 (n = 16) serves as a standard and received a basal diet supplemented with nutritional supplements. The findings showed that the concentration of serum aspartate aminotransferase, alanine aminotransferase, cystatin C, and troponin I after consuming the experimental diets was not statistically different between groups (p > 0.05). The concentrations of mercury (0.000036 mg/kg), lead (0.0047 mg/kg), cadmium (0.00048 mg/kg), and arsenic (0.0046 mg/kg) was very little to cause toxic effect and the levels were below the European Communities Commission (EC) recommended maximum heavy metal levels in foodstuffs. Therefore, consumption of Spirulina at a proportion of 15% does not exert any hepatic, renal, and cardiac toxicities in the mice. However, evaluating the safety of higher doses (> 15%) is required

In vivo evaluation of Spirulina platensis for nutrient bioavailability in mice

This research article published by Indian Journal, 2021Spirulina, a photosynthetic blue-green alga (cyanobacterium), has drawn attention as a viable food supplement due to its suitable nutrient content. Despite its suitable nutrient composition, the bioavailability of nutrients Present in Spirulina is not well reported. In this study, the bioavailability of nutrients present in locally cultivated Spirulina platensis was evaluated by using in vivo method. A total of 54 mice, 5-8 weeks age were used. The mice were randomly divided into three groups. Group 1 (n = 18) served as a control and received a basal diet. Group 2 (n = 20) served as a test and received Spirulina blended with a basal diet. Group 3 (n = 16) serves as a standard and received a basal diet supplemented with nutritional supplements. The study revealed that test diet had apparent absorption of protein 67%, calcium 50.6%, iron 43.8%, zinc 42%, and vitamin A 56.5%, which was higher (p 0.05) with standard diet. Given the higher bioavailability of nutritional supplements mixed into the standard diet, the resemblance in nutrient absorption between test and standard diets illustrated that Spirulina mixed into the test diet also has higher nutrient absorption