Formulation and Characterization of Phytostanol Ester Solid Lipid Nanoparticles for the Management of Hypercholesterolemia: An ex vivo Study.

Background: Phytostanols are naturally occurring compounds that reduce blood cholesterol levels significantly. However, their aqueous insolubility poses formulation challenges. Aim: To formulate and characterize solid lipid nanoparticle carriers for phytostanol esters to enhance the bioavailability of phytostanols. Methods: Phytostanol ester solid lipid nanoparticles were formulated by the microemulsion method. They were characterized for particle size distribution, polydispersity index, shape, surface charge, entrapment efficiency, stability, chemical structure, and thermal properties. The uptake of the formulation by cell lines, HepG2 and HT-29, and its effect on cell viability were evaluated. Results: The formulation of solid lipid nanoparticles was successfully optimised by varying the type of lipids and their concentration relative to that of surfactants in the present study. The optimised formulation had an average diameter of (171 ± 9) nm, a negative surface charge of (− 23.0 ± 0.8) mV and was generally spherical in shape. We report high levels of drug entrapment at (89 ± 5)% in amorphous form, drug loading of (9.1 ± 0.5)%, nanoparticle yield of (67 ± 4)% and drug excipient compatibility. The biological safety and uptake of the formulations were demonstrated on hepatic and intestinal cell lines. Conclusion: Phytostanol ester solid lipid nanoparticles were successfully formulated and characterized. The formulation has the potential to provide an innovative drug delivery system for phytostanols which reduce cholesterol and have a potentially ideal safety profile. This can contribute to better management of one of the main risk factors of cardiovascular disease

Finding the right fit: Enhancing the academic-industry link in the sector for Nutrition undergraduates – a pilot study

Academic learning experience prepares students for professional life, enriches their scientific-evidence knowledge, whereas laboratory practicals upskill their experiences applying theory into “real world” scenarios. As most undergraduate programmes are not offering placement year, students rely heavily on their initiatives and networking to maximise their continuous professional development (CPD). This study evaluated the supporting mechanisms between academia and industry/ sector and examined staff and students’ perceptions of existing academia-industry collaborations. An online survey was designed to record perceptions of undergraduate’s nutrition students. This was followed by focus groups to establish students’ perceptions of the relevant professional organisations and their related experiences outside academia. Captured students’ feedback together with the nutrition teaching academics responses in individual semi-structured interviews have portrayed the current academic-industry links, the perceived challenges/barriers and probed sensible roadmap. Six themes uncovered the need for extra nutrition-related work experiences, while the students’ perceptions reflected their learning through course progression, awareness of external opportunities and underpinned that graduate readiness improved progressively with years spent in study. The Academics’ interviews recognized the limited academic-industry collaborations and underpinned many barriers faced; more “top-down” support was identified as a strategy to enhance external links. The study provides a clear lens into the present academic-industry links within the nutrition programmes and ascertained the perceived challenges experienced by students and academics. Collaborations and centralised university communications shall promote a better university experience. Equally, staff-student partnerships will facilitate a new approach to understand both staff and students’ perspectives and enhance learning experiences within the sector

High omega arachidonic acid/docosahexaenoic acid ratio induces mitochondrial dysfunction and altered lipid metabolism in human hepatoma cells

Background Non-alcoholic fatty liver disease (NAFLD) is a common cause of liver disease worldwide and is a growing epidemic. A high ratio of omega-6 fatty acids to omega-3 fatty acids in the diet has been implicated in the development of NAFLD. However, the inflicted cellular pathology remains unknown. A high ratio may promote lipogenic pathways and contribute to ROS-mediated damage, perhaps leading to mitochondrial dysfunction. Therefore, these parameters were investigated to understand their contribution to NAFLD development. Aim To examine the effect of increasing ratios of omega-6:3 fatty acids on mitochondrial function and lipid metabolism mediators. Methods HepG2-derived VL-17A cells were treated with normal (1:1, 4:1) and high (15:1, 25:1) ratios of omega-6: omega-3 fatty acids (arachidonic (AA): docosahexaenoic (DHA)) at various time points. Mitochondrial activity and function was examined via MTT assay and Seahorse XF24 analyzer, respectively. Triglyceride accumulation was determined by using EnzyChrom™ and levels of ROS were measured by fluorescence intensity. Protein expression of the mediators of lipogenic, lipolytic and endocannabinoid pathways was assessed by Western blotting. Results High AA:DHA ratio decreased mitochondrial activity (p<0.01; upto 80%) and promoted intracellular triglyceride accumulation (p<0.05; 40-70%). Mechanistically, it altered the mediators of lipid metabolism; increased the expression of stearoyl-CoA desaturase (p<0.05; 22-35%), decreased the expression of peroxisome proliferator-activated receptor-alpha (p<0.05; 30-40%) and increased the expression of cannabinoid receptor 1 (p<0.05; 31%). Furthermore, the high ratio increased ROS production (p<0.01; 74-115%) and reduced mitochondrial respiratory functions such as basal and maximal respiration, ATP production, spare respiratory capacity and proton leak (p<0.01; 35-68%). Conclusion High AA:DHA ratio induced triglyceride accumulation, increased oxidative stress and disrupted mitochondrial functions. Stimulation of lipogenic and steroidal transcription factors may partly mediate these effects and contribute to NAFLD development

Risk Factors for Maternal Vitamin D Deficiency within the United Arab Emirates

Introduction: Vitamin D deficiency during pregnancy is a public health problem and it has been associated with negative pregnancy outcomes for both mothers and infants. Aim: To estimate the prevalence of vitamin D deficiency in pregnant women in the United Arab Emirates (UAE) and to identify the contribution of risk factors to the 25(OH)D levels. Methods: It is a cross-sectional study in which vitamin D levels of 1088 adult pregnant women were assessed. Information on vitamin D intake was available in a sub-sample of 266 women. Results: The mean serum 25(OH)D was 26.2 nmol/L (95% CI 25.2-27.1 range 5-129.1 nmol/L) with 69% of women being vitamin D deficient (<30 nmol/L). In the bivariate analysis, showed that no predictors could have been indicated as no values exceeded significance (p<0.2). Stepwise multiple linear regression analysis could not be applied to identify predictors of vitamin D levels as no values exceeded p=0.2. Conclusion: Due to the high prevalence of vitamin deficiency in UAE, there is an urge for interventions focusing on supplementation, fortification and diet diversity for preventing health consequences during a critical period of development

REVE 2021: 9th International Workshop on Reverse Variability Engineering

Software Product Line (SPL) migration remains a challenging endeavour. From organizational issues to purely technical challenges, there is a wide range of barriers that complicates SPL adoption. This workshop aims to foster research about making the most of the two main inputs for SPL migration: 1) domain knowledge and 2) legacy assets. Domain knowledge, usually implicit and spread across an organization, is key to define the SPL scope and to validate the variability model and its semantics. At the technical level, domain expertise is also needed to create or extract the reusable software components. Legacy assets can be, for instance, similar product variants (e.g., requirements, models, source code, etc.) that were implemented using ad-hoc reuse techniques such as clone-and-own. More generally, the workshop REverse Variability Engineering attracts researchers and practitioners contributing to processes, techniques, tools, or empirical studies related to the automatic, semi-automatic or manual extraction or refinement of SPL assets

Management of free flap failure in head and neck surgery

Lutilizzo dei lembi liberi è oggi considerata lopzione di prima scelta nella ricostruzione dei difetti testa-collo, con una percentuale di successo di circa il 95%. La gestione del fallimento di un lembo libero e quale soluzione, tra un secondo lembo libero e un lembo peduncolato, sia più sicura è ancora controversa. Lobiettivo del presente lavoro è descrivere le opzioni adottate dagli Autori e confrontare le scelte e i risultati ottenuti con quelli riportati in letteratura. Dal Gennaio 2012 al Gennaio 2016, presso lUO di Chirurgia Maxillo-Facciale dellOspedale Casa Sollievo della Sofferenza, sono stati allestiti 149 lembi liberi per la ricostruzione di difetti interessanti il distretto testacollo. Di questi, 6 lembi sono stati persi a causa della comparsa di una necrosi totale nel post-operatorio. In 5 casi si è scelto di allestire un secondo lembo libero, nel restante paziente invece è stato utilizzato un lembo di muscolo temporale. Tutti i lembi liberi di salvataggio allestiti hanno avuto successo, senza complicanze e con un buon recupero estetico e funzionale dei pazienti. Analizzando i dati ottenuti e confrontandoli con quanto riportato in letteratura, è possibile concludere come lallestimento di un secondo lembo libero costituisca una procedura sicura e ideale come salvataggio dopo necrosi totale di un precedente lembo

Optical Propagation and Communication

Contains summary of research and reports on four research projects.National Science Foundation (Grant ECS81-20637)U.S. Navy - Office of Naval Research (Contract N00014-81-K-0662)Maryland Procurement Office (Contract MDA904-84-C-6037)U.S. Army Research Office - Durham (Contract DAAG29-80-K-0022)U.S. Army Research Office - Durham (Contract DAAG29-84-K-0095)U.S. Navy - Office of Naval Research (Contract N00014-80-C-0941

Optical Propagation and Communication

Contains reports on four research projects.National Science Foundation (Grant ECS81-20637)U.S. Navy - Office of Naval Research (Contract N00014-81-K-0662)U.S. Army Research Office - Durham (Contract DAAG29-80-K-0022)U.S. Navy - Office of Naval Research (Contract N00014-80-C-0941