Distributed intelligent control and management (DICAM) applications and support for semi-automated development

We have recently begun a 4-year effort to develop a new technology foundation and associated methodology for the rapid development of high-performance intelligent controllers. Our objective in this work is to enable system developers to create effective real-time systems for control of multiple, coordinated entities in much less time than is currently required. Our technical strategy for achieving this objective is like that in other domain-specific software efforts: analyze the domain and task underlying effective performance, construct parametric or model-based generic components and overall solutions to the task, and provide excellent means for specifying, selecting, tailoring or automatically generating the solution elements particularly appropriate for the problem at hand. In this paper, we first present our specific domain focus, briefly describe the methodology and environment we are developing to provide a more regular approach to software development, and then later describe the issues this raises for the research community and this specific workshop

Mach-Zehnder Interferometry in a Strongly Driven Superconducting Qubit

We demonstrate Mach-Zehnder-type interferometry in a superconducting flux qubit. The qubit is a tunable artificial atom, whose ground and excited states exhibit an avoided crossing. Strongly driving the qubit with harmonic excitation sweeps it through the avoided crossing two times per period. As the induced Landau-Zener transitions act as coherent beamsplitters, the accumulated phase between transitions, which varies with microwave amplitude, results in quantum interference fringes for n=1...20 photon transitions. The generalization of optical Mach-Zehnder interferometry, performed in qubit phase space, provides an alternative means to manipulate and characterize the qubit in the strongly-driven regime.Comment: 14 pages, 6 figure

An electrospray ionization study of some novel alkylamine thiohydantoin amino acid derivatives

An electrospray ionization-mass spectrometric (ES-MS) study of some novel alkylamine thiohydantoin amino acid derivatives is presented. The alkylamine derivatives are being developed as part of an on-going effort to couple an Edmanlike protein sequencer to a bench-top electrospray ionization (ES) mass spectrometer. The ES-MS and capillary-skimmer collision-induced dissociation (CID) mass spectra of eight dimethylaminopropylthiohydantoin (DMAP-TH) amino acid derivatives as well as the trimethylaminopropylthiohydantoin (TMAP-TH), diethylaminopropylthiohydantoin, and dibutylaminopropylthiohydantoin (DBAP-TH) derivatives of Phe are presented. The spectra contain prominent [M + H]+ ions as well as fragment ions due to the loss of the respective neutral alkylamines. The CID spectrum of DMAP-TH-Phe also contains the dibutylaminoethyl cation. The relative responses of the alkylamine thiohydantoin derivatives obtained under identical solvent conditions are found to increase as the solvophobicity of the amino acid R group increases; the most solvophobic DBAP-TH-Phe gives rise to the highest overall response. DMAP-TH-Phe and the quaternary amine derivative TMAP-TH-Phe have comparable sensitivities when a sufficiently acidic solvent (pH = 3) is employed. Implications of these studies in the coupling of a protein sequencer to an ES mass spectrometer are discussed as are the requisite modifications of a single quadrupole mass spectrometer for ES analyses

Ion liquid chromatography on-a-chip with beads-packed parylene column

A parylene-MEMS ion-exchange Liquid Chromatography (LC) chip is presented here. The chip is integrated with microfluidic I/O ports, a separation column, frits/filters, and a conductivity detector. The column is packed with conventional LC stationary phase support materials, i.e. micro-beads with surface functional groups. To withstand high pressure normally encountered in high performance liquid chromatography (HPLC), a self-aligned, channel-anchoring technique is developed to increase the pressure rating of the parylene microfluidic devices from 30 to at least 800psi. On-chip injection, separation and detection of anions in water, with ~25 ppm concentration, have been successfully demonstrated. To our knowledge, this is the first demonstration of microbeads-packed column ion liquid Chromatography (LC) on a chip

Purification, Identification, Concentration and Bioactivity of (Z)-7-Dodecen-1-yl Acetate: Sex Pheromone of the Female Asian Elephant, Elephas maximus

In their natural ecosystems, adult male and female Asian elephants, Elephas maximus, live separately. For several weeks prior to ovulation, female elephants release a substance in their urine which elicits a high frequency of non-habituating chemosensory responses, especially flehmen responses, from male elephants. These responses occur prior to, and are an integral part of, mating. Using bioassay-guided fractionation, quantitatively dependent on these chemosensory responses, a specific sex pheromone was isolated and purified by an alternating series of organic and/or aqueous extractions, column chromatography, gas chromatography and high-performance liquid chromatography. Using primarily 1H-proton nuclear magnetic resonance (NMR) spectrometry and gas chromatography-mass spectrometry (GC-MS) of the urine-derived pheromone and its dimethyl disulfide derivative, we determined the structure of the active compound to be (Z)-7-dodecen-1-yl acetate (Z7-12:Ac). Concentrations of Z7-12:Ac in the female urine increased from non-detectable during the luteal phase to 0.48μg/ml (0.002 mM) early in the follicular phase and to 33.0μg/ml (0.146 mM) just prior to ovulation. Bioassays with commercially available authentic synthetic Z7-12:Ac, using 10 Asian male elephants at several locations in the US, demonstrated quantitatively elevated chemosensory responses that were robust during successive tests, and several mating-associated behaviors. Bioassays with Z7-12:Ac with adult male elephants dwelling in more natural social situations in forest camps in Myanmar revealed some differing contextual pre-mating behavioral components. The remarkable convergent evolution of this compound suggests that compounds identified in mammalian exudates that are also present in pheromone blends of insects should be re-evaluated as potential mammalian chemosignals. Chem. Senses 22: 417-437, 199

The Initial-Final Mass Relation among White Dwarfs in Wide Binaries

We present the initial-final mass relation derived from 10 white dwarfs in wide binaries that consist of a main sequence star and a white dwarf. The temperature and gravity of each white dwarf was measured by fitting theoretical model atmospheres to the observed spectrum using a $\chi^{2}$ fitting algorithm. The cooling time and mass was obtained using theoretical cooling tracks. The total age of each binary was estimated from the chromospheric activity of its main sequence component to an uncertainty of about 0.17 dex in log \textit{t} The difference between the total age and white dwarf cooling time is taken as the main sequence lifetime of each white dwarf. The initial mass of each white dwarf was then determined using stellar evolution tracks with a corresponding metallicity derived from spectra of their main sequence companions, thus yielding the initial-final mass relation. Most of the initial masses of the white dwarf components are between 1 - 2 M$_{\odot}$. Our results suggest a correlation between the metallicity of a white dwarf's progenitor and the amount of post-main-sequence mass loss it experiences - at least among progenitors with masses in the range of 1 - 2 M$_{\odot}$. A comparison of our observations to theoretical models suggests that low mass stars preferentially lose mass on the red giant branch.Comment: 28 pages, 8 figures, accepted for publication in Ap

Resonant Readout of a Persistent Current Qubit

We have implemented a resonant circuit that uses a SQUID as a flux-sensitive Josephson inductor for qubit readout. In contrast to the conventional switching current measurement that generates undesired quasi-particles when the SQUID switches to the voltage state, our approach keeps the readout SQUID biased along the supercurrent branch during the measurement. By incorporating the SQUID inductor in a high-Q resonant circuit, we can distinguish the two flux states of a niobium persistent-current (PC) qubit by observing a shift in the resonant frequency of both the magnitude and the phase spectra. The readout circuit was also characterized in the nonlinear regime to investigate its potential use as a nonlinear amplifier.Comment: 4 pages, 2004 ASC Proceeding

Polymer-based electrospray chips for mass spectrometry

In this paper, we present our development of a MEMS chip with an overhanging polymer microcapillary 2.5 mm in length and with a 5 µm x 10 µm orifice size at the tip. The fabricated chips have been successfully interfaced with a mass spectrometer (MS) to validate electrospray ionization (ESI) for biochemical analysis. The prediction of a reduction in Taylor cone size has also been observed with real time ESI fluid visualization from our chip. Built-in micro particle filters and centimeter long serpentine microchannels were fabricated on the chip with a low temperature process by using the Parylene polymer as a structural material, aluminum and photoresist as sacrificial layers, and bromine trifluoride (BrF_3) gas phase etching for final microcapillary releasing. The use of an overhanging polymer structure adds a new a level of mechanical robustness that was never achievable with other thin films. Functionality of our device was proven by consistent detection of myoglobin in a 200 nM solution at a flow rate of 35 nL/min and a voltage potential of 1.5 kV. This MS interface chip represents vital and significant improvements in MEMS process technology and MS functionality with respect to the silicon nitride (Si_xN_y) ESI nozzles previously reported