Capillary-assembled microchip as an on-line deproteinization device for capillary electrophoresis

A capillary-assembled microchip (CAs-CHIP), prepared by simply embedding square capillaries in a lattice polydimethylsiloxane (PDMS) channel plate with the same channel dimensions as the outer dimensions of the square capillaries, has been used as a diffusion-based pretreatment attachment in capillary electrophoresis (CE). Because the CAs-CHIPs employ square-section channels, diffusion-based separation of small molecules from sample solutions containing proteins is possible by using the multilayer flow formed in the square section channel. When a solution containing high-molecular-weight and low-molecular-weight species makes contact with a buffer solution, the low-molecular-weight species, which have larger diffusion coefficients than the high-molecular-weight species, can be collected in a buffer-solution phase. The collected solution containing the low-molecular-weight species is introduced into the separation capillary to be analyzed by CE. This type of system can be used for CE analysis in which pretreatment is required to remove proteins. In this work a fluorescently labeled protein and rhodamine-based molecules were chosen as model species and a feasibility study was performed. [Image: see text

カガクコウゾウオヨビハンノウキコウノモンダイニタイスルESRブンコウガクノオウヨウ

京都大学0048新制・論文博士工学博士乙第2316号論工博第607号新制||工||251(附属図書館)3733UT51-48-E666(主査)教授 舟阪 渡, 教授 松浦 輝男, 教授 野崎 一学位規則第5条第2項該当Kyoto UniversityDA