1 research outputs found
Assessment of resolution and intercenter reproducibility of results of genotyping Staphylococcus aureus by pulsed-field gel electrophoresis of SmaI macrorestriction fragments: a multicenter study
Twenty well-characterized isolates of methicillin-resistant Staphylococcus
aureus were used to study the optimal resolution and interlaboratory
reproducibility of pulsed-field gel electrophoresis (PFGE) of DNA
macrorestriction fragments. Five identical isolates (one PFGE type), 5
isolates that produced related PFGE subtypes, and 10 isolates with unique
PFGE patterns were analyzed blindly in 12 different laboratories by
in-house protocols. In several laboratories a standardized PFGE protocol
with a commercial kit was applied successfully as well. Eight of the
centers correctly identified the genetic homogeneity of the identical
isolates by both the in-house and standard protocols. Four of 12
laboratories failed to produce interpretable data by the standardized
protocol, due to technical problems (primarily plug preparation). With the
five rel