3 research outputs found
Determination of DNA Damage in Floriculturists Exposed to Mixtures of Pesticides
The aim of the study was to determine possible DNA damage in floriculturists chronically exposed to pesticides. Leukocytes from 52 workers, 46 environmentally exposed, and 38 control individuals were evaluated with the comet assay. Serum from all individuals was also analyzed for pesticides using gas chromatography coupled to mass spectrometry. A statistically significant difference in DNA fragmentation in the pesticide exposed group compared to the other two groups (P < .001) was found. No differences between environmentally exposed and control individuals were detected. The statistical analysis showed no significant correlation between DNA damage and sex, age, drinking or smoking habits, as well as years of exposure. One or more pesticides were detected in 50% of the floriculturists, while in the rest of the individuals, a chemical related with the preparation of pesticides, such as additives, plasticizers, or solvents, was found. Our study shows that chronic exposure to pesticides produces DNA damage in floriculturists. It also suggests that this type of monitoring could be valuable in recommending preventive measures
Phylogeny of the Neotropical sages (Salvia subg. Calosphace; Lamiaceae) and insights into pollinator and area shifts
Salvia subg. Calosphace (Lamiaceae, Lamiales) is a highly diverse clade endemic to the New World. The phylogenetic relationships of Calosphace have been previously investigated using DNA sequences of nuclear ITS region and plastid psbA–trnH intergenic spacer, but the resulting trees lack resolution and support for many clades. The present paper reassesses the phylogenetic relationships of subgenus Calosphace, including a broader taxon sampling, with a special focus on representing previously unsampled sections, and using an additional plastid marker (trnL–trnF region). Our results show increased resolution and overall patterns of support, recovering ten main clades. Within core Calosphace, the most inclusive of these main clades, 17 new subclades were identified. Of the 42 sections for which more than one species was analysed, only 12 are monophyletic. Our biogeographical analysis identified at least twelve migrations to South America from Mexican and Central American lineages, in agreement with previous suggestions of multiple origins of South American Calosphace diversity. This analysis also confirmed a colonization of the Antilles by Andean lineages. The reconstruction of ancestral states of pollination syndromes showed multiple shifts to ornithophily from melittophily and one reversal to the latter.Fil: Fragoso-MartĂnez, Itzi. Universidad Nacional AutĂłnoma de MĂ©xico; MĂ©xicoFil: MartĂnez-Gordillo, Martha. Universidad Nacional AutĂłnoma de MĂ©xico; MĂ©xicoFil: Salazar, Gerardo A.. Universidad Nacional AutĂłnoma de MĂ©xico; MĂ©xicoFil: Sazatornil, Federico David. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - CĂłrdoba. Instituto Multidisciplinario de BiologĂa Vegetal. Universidad Nacional de CĂłrdoba. Facultad de Ciencias Exactas FĂsicas y Naturales. Instituto Multidisciplinario de BiologĂa Vegetal; ArgentinaFil: Jenks, Aaron A.. Berkeley University; Estados UnidosFil: GarcĂa Peña, MarĂa del Rosario. Universidad Nacional AutĂłnoma de MĂ©xico; MĂ©xicoFil: Barrera-Aveleida, Giovanna. Universidad Nacional AutĂłnoma de MĂ©xico; MĂ©xicoFil: Benitez Vieyra, Santiago Miguel. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - CĂłrdoba. Instituto Multidisciplinario de BiologĂa Vegetal. Universidad Nacional de CĂłrdoba. Facultad de Ciencias Exactas FĂsicas y Naturales. Instituto Multidisciplinario de BiologĂa Vegetal; ArgentinaFil: MagallĂłn, Susana. Universidad Nacional AutĂłnoma de MĂ©xico; MĂ©xicoFil: Cornejo-Tenorio, Guadalupe. Universidad Nacional AutĂłnoma de MĂ©xico; MĂ©xicoFil: Granados Mendoza, Carolina. Universidad Nacional AutĂłnoma de MĂ©xico; MĂ©xic