Crack Mechanism of Different Concrete Grades under Compressive Loading: Application in Rigid Pavement

The growth of cracks in concretes under different loadings in various structures and their consequent diminishing effect on the lifespan of structures is an age long structural problem. This research investigates the influence of compressive loading on the crack mechanism of different concrete grades 1: 1 : 3 (M20), 1: 2: 4 (M15) and 1: 3: 6 (M10) using three brands of cements available in Nigeria namely Dangote (Type A), Elephant (Type B) and Bua (Type C). Particle Size Distribution (PSD), Aggregate Crushing Value (ACV), Aggregate Impact Value (AIV) was carried out on aggregates in accordance with relevant codes while; compressive test were performed on the hardened concrete while, the crack width was measured using microscopic technique. A coefficient of uniformity (Cu) of 4.00, AIV of 46 % and an ACV of 47.7 % were obtained for the aggregate which are suitable for rigid pavements construction. Maximum compressive strength value of 24.96 N/mm2 , 24.67 N/mm2 and 24.89 N/mm2 respectively was obtained for concrete M20 for Type A, Type B and Type C cement at the 28 day. A corresponding crack width of 0.97 mm, 0.89 mm and 0.93 mm was obtained while; a yield period of 57.66 sec, 58.33 sec and 53.33 was obtained respectively. Comparing with the International Organization for Standardization, concrete grade M20 any of the cement types is suitable for the construction of rigid pavements with heavy traffic volume while M15 is recommended for low traffic volume

Immunoinformatics assisted design of a multi-epitope kit for detecting <i>Cronobacter sakazakii</i> in powdered infant formula

Objectives: Cronobacter sakazakii, formerly Enterobacter sakazakii, is an emerging ubiquitous and opportunistic foodborne pathogen with a high mortality rate. It has been implicated in cases of meningitis, septicaemia, and necrotizing enterocolitis among infants worldwide in association with powdered infant formula (PIF). This study was an insilico designed peptide base kit framework, using immunoinformatic techniques for quick detection of C. sakazakii in PIF. Materials and Methods: In the present study, a peptide-based kit was designed with a bioinformatic technique to rapidly identify C. sakazakii in PIF using flhE, secY, and bcsC, which are genes responsible for its biofilm formation, as target genes. The antigenicity, membrane topology, and the presence of signal peptides of the target genes were analysed using VaxiJen, DeepTMHMM, and SignalP servers. To provide stability and flexibility to the multiple-epitope construct, the linear B cells and helper T cells (IL-4 (interleukin 4) and IL-10 (interleukin 10) inducing epitopes) were linked with a GSGSG linker followed by the addition of protein disulphide bonds. To ascertain specificity, the multi-epitope construct was molecularly docked against genes from sources other than PIF, like alfalfa, and the environment, with PIF being the highest: –328.48. Finally, the codons were modified using the pET28a(+) vector, and the resultant multi-epitope construct was successfully cloned in silico. Results: The final construct had a length of 486 bp, an instability index of 23.26, a theoretical pI of 9.34, a molecular weight of 16.5 kDa, and a Z-score of –3.41. Conclusions: The multi-epitope peptide construct could be a conceptual framework for creating a C. sakazakii peptide-based detection kit, which has the potential to provide fast and efficient detection. However, there is a need for additional validation through the in vitro and in vivo techniques

Biological Activity of Selected Compounds from Annona muricata Seed as Antibreast Cancer Agents: Theoretical Study

Several natural products have been of help to humans, and its effect is noticeable in the medicinal world. Soursop with botanical name Annona muricata L. possesses antidiarrhea, anticold fever, antirheumatism, and antineuralgia properties. In this work, five selected molecular compounds were studied against type 3 of 3α-hydroxysteroid dehydrogenase (3α-HSD). Its anticancer activity was investigated using the quantum chemical method via Spartan 14 software, molecular docking via Discovery studio 2017, AutoDock Tool 1.5.6, AutoDock Vina 1.1.2, and PyMol 1.7.4.4 and the molecular dynamic simulation method via AMBER14 molecular dynamics package. Many descriptors (EHOMO, ELUMO, dipole moment, energy bandgap, area, volume, polarizability, polar surface area, Log P, hydrogen bond donor, and hydrogen bond acceptor) which describe the anticancer activity of the studied compounds were obtained. Also, the docking study revealed the inhibiting ability of the studied compound, and it was observed that compound C possesses a greater ability to inhibit than other studied compounds as well as the standard (5FU)

Oral fetus-in-fetu: A case report

Fetus-in-fetu is a rare condition, less than 200 cases have been reported. Its embryopathogenesis is linked to a monozygotic, diamniotic parasitic twin. The presence of a calcified vertebral column and other body parts are key to the diagnosis, and differentiate it from a teratoma. We report a case of a neonate who was admitted immediately after delivery by Caesarian section following a prolonged obstructed labor caused by a huge mass projecting from the hard palate. The mass had identifiable malformed body parts but was anencephalic. Intraoperative findings were a short stalk and cleft of the soft palate. He had excision of the mass and did well post operatively. Persistent mouth breathing and difficult nasal cannulation lead to request for post operative magnetic resonance imaging which showed patent nostrils and absent residual mass. Prompt and skillful anesthesia and surgical intervention assisted in the survival of this patient