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Renal effects of Mammea africana Sabine (Guttiferae) stem bark methanol/methylene chloride extract on L-NAME hypertensive rats
Objective : The present study aims at evaluating the effects of
methanol/methylene chloride extract of the stem bark of Mammea africana
on the renal function of L-NAME treated rats. Material and Methods :
Normotensive male Wistar rats were divided into five groups
respectively treated with distilled water, L-NAME (40 mg/kg/day),
L-NAME + L-arginine (100 mg/kg/day), L-NAME + captopril (20 mg/kg/day)
or L-NAME + M. africana extract (200 mg/kg/day) for 30 days. Systolic
blood pressure was measured before and at the end of treatment. Body
weight was measured at the end of each week. Urine was collected 6 and
24 h after the first administration and further on day 15 and 30 of
treatment for creatinine, sodium and potassium quantification, while
plasma was collected at the end of treatment for the creatinine assay.
ANOVA two way followed by Bonferonni or one way followed by Tukey were
used for statistical analysis. Results : M. africana successfully
prevented the rise in blood pressure and the acute natriuresis and
diuresis induced by L-NAME. When given chronically, the extract
produced a sustained antinatriuretic effect, a non-significant increase
in urine excretion and reduced the glomerular hyperfiltration induced
by L-NAME. Conclusions : The above results suggest that the
methanol/methylene chloride extract of the stem bark of M. africana may
protect kidney against renal dysfunction and further demonstrate that
its antihypertensive effect does not depend on a diuretic or
natriuretic activity