VARIAÇÃO ESPACIAL DOS ATRIBUTOS FÍSICOS E ESTOQUES DE CARBONO DO SOLO EM PLANTIO DE Schizolobium amazonicum NA AMAZÔNIA

Tree plantations provide an increase in soil carbon stocks, but studies involving native Amazonian species are incipient. The objective was to evaluate the spatial variation of physical attributes and soil organic carbon stock in a paricá plantation. In the study area, soil samples were collected at depths of 0 to 20 cm and 20 to 40 cm and the parameters analyzed were: soil texture, macroporosity, microporosity, soil bulk density, mechanical soil resistance to penetration, and soil organic carbon content and stocks. The data were interpolated by the inverse of the square of the distance. The spatial variation of soil physical attributes at planting was influenced by texture. There was a low range of spatial variation in soil organic carbon content and stocks. Paricá planting was efficient in fixing soil carbon at depth.Plantios de árvores proporcionam aumento dos estoques de carbono do solo, mas estudos envolvendo espécies nativas da Amazônia são incipientes. Objetivou-se avaliar a variação espacial dos atributos físicos e estoque de carbono orgânico no solo em plantio de paricá. Na área de estudo, coletaram-se amostras de solo nas profundidades de 0 a 20 cm e de 20 a 40 cm e os parâmetros analisados foram: textura do solo, macroporosidade, microporosidade, densidade aparente do solo, resistência mecânica do solo à penetração, teores e os estoques de carbono orgânico do solo. Interpolou-se os dados pelo inverso do quadrado da distância. A variação espacial dos atributos físicos do solo no plantio foi influenciada pela textura. Houve baixa amplitude da variação espacial dos teores e estoques de carbono orgânico do solo. O plantio de paricá apresentou eficiência na fixação de carbono no solo em profundidade. Palavras-chave: silvicultura; sequestro de carbono; física do solo; mudança climática.   Spatial variation of physical attributes and soil carbon stocks in a Schizolobium amazonicum plantation in the amazon   ABSTRACT: Tree plantations provide an increase in soil carbon stocks, but studies involving native Amazonian species are incipient. The objective was to evaluate the spatial variation of physical attributes and soil organic carbon stock in a paricá plantation. In the study area, soil samples were collected at depths of 0 to 20 cm and 20 to 40 cm and the parameters analyzed were: soil texture, macroporosity, microporosity, soil bulk density, mechanical soil resistance to penetration, and soil organic carbon content and stocks. The data were interpolated by the inverse of the square of the distance. The spatial variation of soil physical attributes at planting was influenced by texture. There was a low range of spatial variation in soil organic carbon content and stocks. Paricá planting was efficient in fixing soil carbon at depth. Keywords: silviculture; carbon sequestration; soil physics; climate change

Prevention of Sudden Cardiac Death in Hypertrophic Cardiomyopathy: What has Changed in The Guidelines?

Abstract Background: The new European Society of Cardiology guidelines for hypertrophic cardiomyopathy (HCM) define the estimation of sudden cardiac death (SCD) risk as an integral part of clinical management. An implantable cardioverter defibrillator (ICD) is recommended (class IIa) when the risk is ≥ 6%. Objectives: To compare the SCD risk stratification according to the 2011 and 2014 recommendations for ICD implantation in patients with HCM. Methods: Retrospective study including 105 patients diagnosed with HCM. The indication for ICD was assessed using the 2011 and 2014 guidelines. Statistical analysis was performed using SPSS software version 19.0.0.2®. The tests performed were bilateral, considering the significance level of 5% (p < 0.05). Results: Regarding primary prevention, according to the 2011 ACCF/AHA recommendations, 39.0% of the patients had indication for ICD implantation (level of evidence IIa). Using the 2014 guidelines, only 12.4% of the patients had an indication for ICD implantation. Comparing the two risk stratification models for patients with HCM, we detected a significant reduction in the number of indications for ICD implantation (p < 0.001). Of the 41 patients classified as IIa according to the 2011 recommendations, 68.3% received a different classification according to the 2014 guidelines. Conclusion: Significant differences were found when comparing the SCD risk stratification for ICD implantation in the two guidelines. The current SCD risk score seems to identify many low-risk patients who are not candidates for ICD implantation. The use of this new score results in a significant reduction in the number of ICD implanted

Biochemical and Microbiological Aspects of the Oral Cavity of Children and Young People with Neurological Impairment and Oropharyngeal Dysphagia

The components and the salivary flow have a direct influence on the composition of the oral microbiota of children and young people with oropharyngeal dysphagia, and studies have already demonstrated the excessive accumulation of supragingival dental calculus in individuals with enteral nutrition. This study aimed to compare the oral hygiene, biochemical, and microbiological aspects of the oral cavity of children and young people with neurological impairment and oropharyngeal dysphagia. Forty children and young people with neurological impairment and oropharyngeal dysphagia were enrolled and divided into two groups: group I, encompassing 20 participants fed via gastrostomy; and group II, encompassing 20 participants fed via the oral route. Oral hygiene and salivary pH and flow were assessed, and a polymerase chain reaction was performed to evaluate the messenger RNA expressions of Porphyromonas gingivalis, Tanerella forsythia, and Treponema denticola. In groups I and II, the mean Oral Hygiene Index—Simplified scores were 4 and 2, respectively, showing a significant difference; the mean Calculus Index scores were 2 and 0, respectively, showing a significant difference; and the mean pH was 7.5 and 6.0, respectively, showing a significant difference. Bacterial analysis indicated no association between the two groups. It can be concluded that children and young people who use gastrostomy had a poorer oral hygiene, greater dental calculus deposition, and higher salivary pH. The saliva of patients in both groups contained Porphyromonas gingivalis, Tanerella forsythia, and Treponema denticola

Parasite Detection in Visceral Leishmaniasis Samples by Dye-Based qPCR Using New Gene Targets of <i>Leishmania infantum</i> and <i>Crithidia</i>

Visceral leishmaniasis (VL) is a neglected disease considered a serious public health problem, especially in endemic countries. Several studies have discovered monoxenous trypanosomatids (Leptomonas and Crithidia) in patients with VL. In different situations of leishmaniasis, investigations have examined cases of co-infection between Leishmania spp. and Crithidia spp. These coinfections have been observed in a wide range of vertebrate hosts, indicating that they are not rare. Diagnostic techniques require improvements and more robust tools to accurately detect the causative agent of VL. This study aimed to develop a real-time quantitative dye-based PCR (qPCR) assay capable of distinguishing Leishmania infantum from Crithidia-related species and to estimate the parasite load in samples of VL from humans and animals. The primer LinJ31_2420 targets an exclusive phosphatase of L. infantum; the primer Catalase_LVH60-12060_1F targets the catalase gene of Crithidia. Therefore, primers were designed to detect L. infantum and Crithidia sp. LVH60A (a novel trypanosomatid isolated from VL patients in Brazil), in samples related to VL. These primers were considered species-specific, based on sequence analysis using genome data retrieved from the TriTryp database and the genome assembling of Crithidia sp. LVH60A strain, in addition to experimental and clinical data presented herein. This novel qPCR assay was highly accurate in identifying and quantifying L. infantum and Crithidia sp. LVH60A in samples obtained experimentally (in vitro and in vivo) or collected from hosts (humans, dogs, cats, and vectors). Importantly, the screening of 62 cultured isolates from VL patients using these primers surprisingly revealed that 51 parasite cultures were PCR+ for Crithidia sp. In addition, qPCR assays identified the co-infection of L. infantum with Crithidia sp. LVH60A in two new VL cases in Brazil, confirming the suspicion of co-infection in a previously reported case of fatal VL. We believe that the species-specific genes targeted in this study can be helpful for the molecular diagnosis of VL, as well as for elucidating suspected co-infections with monoxenous-like trypanosomatids, which is a neglected fact of a neglected disease

Comenta&#769;rios a uma sentenc&#807;a anunciada : o processo Lula

“Comentários a uma sentença: o Caso Lula” é talvez o mais importante documento jurídico publicado no Brasil em décadas. A presente coletânea de artigos nasceu de um movimento espontâneo e bastante significativo de juristas brasileiros e estrangeiros que examinaram cuidadosamente a sentença proferida no âmbito do processo que tramitou na 13ª Vara Federal de Curitiba, no caso que ficou conhecido na mídia como o do “tríplex do Guarujá”. <br>De la presentación de Geraldo Prad