Praktično značenje dijagnostičke mobilizacije olova

I. On the basis of many years\u27 experience with the diagnostic mobilization of lead in out-patients it is recommended to take urine samples six hours after injection. 2. As maximum permissible concentration after the lead mobilization a value within 0,8-1.0 mg/24 hrs (or 0.45-0.56 mg/hrs.) is suggested. 3. With the values exceeding 3 mg/24 hrs. in 900/o of cases at least two positive laboratory tests can be expected. Anemia is most frequently present when values reach 5 mg. 4. This method is recommended for the diagnosis of atypical cases, for the control of treatment and when a decision is to be reached whether a patient ill to return to work with lead.Na temelju dugogodišnjeg iskustva s dijagnostičkom mobilizacijom olova kod ambulantnih pacijenata, preporučuje se uzimanje uzoraka urina šest sati nakon injekcije. Vrijednost u granicama od 0,8-1,0 mg/24 sata {ili 0,45-0,56 mg/6 sati) preporučuje se kao maksimalno dopuštena koncentracija nakon mobilizacije olova. Kad su vrijednosti veće od 3 mg/24 sata mogu se u 90% slučajeva očekivati bar dva pozitivna laboratorijska testa. Anemija se najčešće pojavljuje kad vrijednosti. Ova se metoda može preporučiti za dijagnostiku atipičnih slučajeva, za kontrolu liječenja i kad treba donijeti odluku da li je pacijent sposoban da se ponovo vrati radu s olovom

Excretion of lead and its biological activity several years after termination of exposure

Přerovská, and Teisinger, J. (1970).Brit. J. industr. Med.,27, 352-355. Excretion of lead and its biological activity several years after termination of exposure. A group of 27 persons who had been treated some years previously for chronic lead poisoning at our clinic, and who had not come into occupational contact with lead since, was examined. Half of them had had no occupational exposure to lead for 3 to 5 years and the others for 8 to 17 years. In most of these persons there is still an increased lead excretion, originating from an increased deposit in the bones. The mobilization test after calcium versenate (CaEDTA) injection was greater than 0·350 mg/24 hours. The values found for haemoglobin, punctate basophilia, coproporphyrin and ALA in urine were normal, but there was, in all cases, a decreased ALA-D activity. This finding suggests biological activity of such negligible lead flow many years after termination of exposure

Effect of Tetraethyl Thiuram Disulphide (Disulfiram) on Metabolism of Trichloroethylene in Man

The amounts of trichloroethanol and trichloroacetic acid excreted in the urine of four subjects who inhaled trichloroethylene in a concentration of about 1 mg./l. for a period of five hours in a laboratory experiment were determined. This experiment was repeated under the same conditions after tetraethyl thiuram disulphide (disulfiram) had been given in divided doses, totalling 3 or 3·5 g. The elimination of trichloroethanol in urine was decreased by 40 to 64%, and of trichloroacetic acid by 72 to 87%. The trichloroethylene excreted by the lungs in two of the subjects increased up to 65% of that retained within five hours. It is concluded that tetraethyl thiuram disulphide (disulfiram) strikingly inhibits the oxidation of trichloroethylene. The possible therapeutic use of this substance in cases of severe peroral trichloroethylene intoxication is discussed

Ionic interactions are essential for TRPV1 C-terminus binding to calmodulin

Calmodulin (CaM) is known to play an important role in the regulation of TRP channels activity. Although it has been reported that CaM binds to the C-terminus of TRPV1 (TRPV1-CT), no classic CaM-binding motif was found in this region. In this work, we explored this unusual TRPV1 CaM-binding motif in detail and found that five residues from a putative CaM-binding motif are important for TRPV1-CT's binding to CaM, with arginine R785 being the most essential residue. The homology modelling suggests that a CaM-binding motif of TRPV1-CT forms an alpha helix that docks into the central cavity of CaM. © 2008 Elsevier Inc. All rights reserved