A venous occlusion plethysmography using a load cell as the sensing element

An application of the load cell as a sensor in venous occlusion plethysmography is presented. In this method the limb volume changes that follow venous occlusion are converted into water volume changes using a water tank for volume change detection. The hydrostatic pressure, as well as the water surface level, is measured and used for the calculation of the volume change. By using this method the influence of water pressure on limb blood flow, as well as drift and leakage of the sensing element, is avoided. The load cell has the advantage of measuring the weight of the displaced water volume, which simplifies the design principles of the plethysmography. The plethysmography is found to be sensitive, highly linear, and easy to handle. It has been evaluated in several subjects, and the results of these studies are in agreement with earlier results </p

Mass spectrometry of hydrogen/deuterium exchange in 70S ribosomal proteins from E. coli

AbstractThe 70S ribosome from Escherichia coli is a supermacro complex (MW: 2.7MDa) comprising three RNA molecules and more than 50 proteins. We have for the first time successfully analyzed the flexibility of 70S ribosomal proteins in solution by detecting the hydrogen/deuterium exchange with mass spectrometry. Based on the deuterium incorporation map of the X-ray structure obtained at the time of each exchange, we demonstrate the structure–flexibility–function relationship of ribosome focusing on the deuterium incorporation of the proteins binding ligands (tRNA, mRNA, and elongation factor) and the relation with structural assembly processes

Significance of Des-gamma-carboxy Prothrombin Production in Hepatocellular Carcinoma

Serum des-gamma-carboxy prothrombin (DCP) is commonly used to detect hepatocellular carcinoma (HCC). This review focuses on the clinical features of DCP-positive HCC and the molecular function of DCP in HCC. DCP-positive HCC demonstrates more aggressive clinicopathological features than DCP-negative HCC. Analysis of the biological effects of DCP revealed that DCP acts as a growth factor in both an autocrine and paracrine manner. DCP stimulates HCC cell proliferation through the Met-Janus kinase 1-signal transducer and activator of transcription 3 signaling pathway, whereas for vascular endothelial cells, it stimulates cell proliferation and migration through the kinase insert domain receptor-phospholipase C-gamma-mitogen-activated protein kinase signaling pathway.</p