8 research outputs found
Transgenic tobacco plants expressing Poxa1b gene are able to reduce olive oil mill wastewater phenols content
Effect of of fungal phenoloxidases expression in plant and green microalgae on olive mill wastewaters phenols content
Fritillaria messanensis subsp. gracilis (Liliaceae), a new record for the Italian flora (S Italy)
Engineered tobacco and microalgae secreting the fungal laccase POXA1b reduce phenol content in olive oil mill wastewater
Green microalgae and plants expressing fungal laccases useful for enviromental and industrial applications
A biotechnological approach to enhance the efficiency of xenobiotic removal from green organisms is to overexpress genes involved in metabolism, uptake or transport of specific organic pollutants. However, a useful approach is to secrete laccases in the soil or water. The laccase cDNA of the poxA1b gene from Pleurotus ostreatus, carrying a signal peptide sequence for enzyme secretion and driven by the CaMV 35S promoter, was cloned into a plant expression vector.
Nuclear genetic transformation was carried out by co-cultivation of Agrobacterium tumefaciens with tobacco cv Samsun NN leaves and cells of five different microalgae accessions belonging to the genera Chlamydomonas, Chlorella and Ankistrodesmus. Transgenic plants and microalgae were able to express and secrete the recombinant laccase in the root exudates and the culture medium, respectively. In comparison to untransformed controls, the ability to reduce phenol content in OMW solution was enhanced up to 2.8-fold in transgenic tobacco lines and by up to about 40% in two microalgae accessions. The present work provides new evidence for metabolic improvement of green organisms through the transgenic approach useful for environmental and industrial applications