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    ΠŸΠ Π˜ΠœΠ•ΠΠ•ΠΠ˜Π• ΠšΠ Π•ΠœΠ Β«Π˜Π—ΠžΠ›Π˜Π€Π’Β» И ΠšΠ Π˜ΠžΠœΠΠ‘Π‘ΠΠ–Π Π”Π›Π― ΠšΠžΠ Π Π•ΠšΠ¦Π˜Π˜ ΠžΠ’Π•Π§ΠΠž-Π€Π˜Π‘Π ΠžΠ‘ΠšΠ›Π•Π ΠžΠ’Π˜Π§Π•Π‘ΠšΠžΠ™ ΠŸΠΠΠΠ˜ΠšΠ£Π›ΠžΠŸΠΠ’Π˜Π˜

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    107 patients with cellulitis were under investigation. Treatment with applications of Isolift cream (Uriage cosmetic line) and cryomassage intensified microcirculation in problem zones, made skin more hydrated, fat and smooth. Complex treatment appeared to be more effective than usage of each of methods itself. Obtained results are due to the cream components effecting main metabolic processes in the skin and subcutaneous fat as well as to the specific effect of cryomassage on microcirculation.ОбслСдовано 107 ΠΏΠ°Ρ†ΠΈΠ΅Π½Ρ‚ΠΎΠΊ с ΠΎΡ‚Π΅Ρ‡Π½ΠΎ-фибросклСротичСской ΠΏΠ°Π½Π½ΠΈΠΊΡƒΠ»ΠΎΠΏΠ°Ρ‚ΠΈΠ΅ΠΉ. ΠŸΡ€ΠΈ ΠΏΡ€ΠΎΠ²Π΅Π΄Π΅Π½ΠΈΠΈ лСчСния с использованиСм Π°ΠΏΠΏΠ»ΠΈΠΊΠ°Ρ†ΠΈΠΉ ΠΊΡ€Π΅ΠΌΠ° Β«Π˜Π·ΠΎΠ»ΠΈΡ„Ρ‚Β» космСтичСской Π»ΠΈΠ½ΠΈΠΈ Β«UriageΒ» ΠΈ криомассаТа выявлСно ΡƒΠ»ΡƒΡ‡ΡˆΠ΅Π½ΠΈΠ΅ микроциркуляции Π² ΠΏΡ€ΠΎΠ±Π»Π΅ΠΌΠ½Ρ‹Ρ… Π·ΠΎΠ½Π°Ρ…, ΡƒΠ²Π΅Π»ΠΈΡ‡Π΅Π½ΠΈΠ΅ влаТности, Тирности ΠΈ гладкости ΠΊΠΎΠΆΠΈ, ΡƒΠΌΠ΅Π½ΡŒΡˆΠ΅Π½ΠΈΠ΅ содСрТания ΠΆΠΈΡ€ΠΎΠ²ΠΎΠΉ Ρ‚ΠΊΠ°Π½ΠΈ. КомплСксноС Π»Π΅Ρ‡Π΅Π½ΠΈΠ΅ оказалось Π±ΠΎΠ»Π΅Π΅ эффСктивным, Ρ‡Π΅ΠΌ ΠΏΡ€ΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ ΠΎΡ‚Π΄Π΅Π»ΡŒΠ½Ρ‹Ρ… ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΈΠΊ.ΠŸΠΎΠ»ΡƒΡ‡Π΅Π½Π½Ρ‹Π΅ Ρ€Π΅Π·ΡƒΠ»ΡŒΡ‚Π°Ρ‚Ρ‹ обусловлСны ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½Ρ‚Π°ΠΌΠΈ ΠΊΡ€Π΅ΠΌΠ°, Π²Π»ΠΈΡΡŽΡ‰ΠΈΠΌΠΈ Π½Π° основныС процСссы ΠΌΠ΅Ρ‚Π°Π±ΠΎΠ»ΠΈΠ·ΠΌΠ° Π² ΠΊΠΎΠΆΠ΅ ΠΈ ΠΏΠΎΠ΄ΠΊΠΎΠΆΠ½ΠΎ-ΠΆΠΈΡ€ΠΎΠ²ΠΎΠΉ ΠΊΠ»Π΅Ρ‚Ρ‡Π°Ρ‚ΠΊΠΈ ΠΈ особСнностями дСйствия криомассаТа, Π°ΠΊΡ‚ΠΈΠ²Π½ΠΎ Π²Π»ΠΈΡΡŽΡ‰Π΅Π³ΠΎ Π½Π° состояниС микроциркуляции

    In vitro fertilization of bovine oocytes in protein-free culture system

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    We studied the possibility of fertilization of bovine oocyte-cumulus complexes, matured in vitro in a protein-free medium, in a protein-free culture system without preliminary capacitation of spermatozoa. The development of embryos to the morula-blastocyst and blastocyst stage was considered as a criterion of successful fertilization. It was shown that replacement of bovine serum albumin for polyvinyl alcohol or polyvinylpyrrolidone in Tyrode medium for fertilization did not affect significantly the development to the morula-blastocyst stage and the number of cells in blastocysts. It was also found that replacement bovine serum albumin for polyvinyl alcohol in all used media, Tyrode medium for washing of oocytes, medium for sperm preparation to fertilization, and Tyrode medium for fertilization, did not affect significantly the development to the morula-blastocyst and blastocyst stages, as well as on the number of cells in blastocysts. The results obtained suggest that in vitro fertilization of bovine oocyte-cumulus complexes is possible in a protein-free culture system without significant reduction in the capacity for in vitro development of the obtained embryos and number of cells in blastocysts
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