2 research outputs found
ΠΠ ΠΠΠΠΠΠΠΠ ΠΠ ΠΠΠ Β«ΠΠΠΠΠΠ€Π’Β» Π ΠΠ ΠΠΠΠΠ‘Π‘ΠΠΠ ΠΠΠ― ΠΠΠ Π ΠΠΠ¦ΠΠ ΠΠ’ΠΠ§ΠΠ-Π€ΠΠΠ ΠΠ‘ΠΠΠΠ ΠΠ’ΠΠ§ΠΠ‘ΠΠΠ ΠΠΠΠΠΠΠ£ΠΠΠΠΠ’ΠΠ
107 patients with cellulitis were under investigation. Treatment with applications of Isolift cream (Uriage cosmetic line) and cryomassage intensified microcirculation in problem zones, made skin more hydrated, fat and smooth. Complex treatment appeared to be more effective than usage of each of methods itself. Obtained results are due to the cream components effecting main metabolic processes in the skin and subcutaneous fat as well as to the specific effect of cryomassage on microcirculation.ΠΠ±ΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΎ 107 ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠΊ Ρ ΠΎΡΠ΅ΡΠ½ΠΎ-ΡΠΈΠ±ΡΠΎΡΠΊΠ»Π΅ΡΠΎΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΏΠ°Π½Π½ΠΈΠΊΡΠ»ΠΎΠΏΠ°ΡΠΈΠ΅ΠΉ. ΠΡΠΈ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ΠΈΠΈ Π»Π΅ΡΠ΅Π½ΠΈΡ Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ Π°ΠΏΠΏΠ»ΠΈΠΊΠ°ΡΠΈΠΉ ΠΊΡΠ΅ΠΌΠ° Β«ΠΠ·ΠΎΠ»ΠΈΡΡΒ» ΠΊΠΎΡΠΌΠ΅ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ Π»ΠΈΠ½ΠΈΠΈ Β«UriageΒ» ΠΈ ΠΊΡΠΈΠΎΠΌΠ°ΡΡΠ°ΠΆΠ° Π²ΡΡΠ²Π»Π΅Π½ΠΎ ΡΠ»ΡΡΡΠ΅Π½ΠΈΠ΅ ΠΌΠΈΠΊΡΠΎΡΠΈΡΠΊΡΠ»ΡΡΠΈΠΈ Π² ΠΏΡΠΎΠ±Π»Π΅ΠΌΠ½ΡΡ
Π·ΠΎΠ½Π°Ρ
, ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΠ΅ Π²Π»Π°ΠΆΠ½ΠΎΡΡΠΈ, ΠΆΠΈΡΠ½ΠΎΡΡΠΈ ΠΈ Π³Π»Π°Π΄ΠΊΠΎΡΡΠΈ ΠΊΠΎΠΆΠΈ, ΡΠΌΠ΅Π½ΡΡΠ΅Π½ΠΈΠ΅ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ ΠΆΠΈΡΠΎΠ²ΠΎΠΉ ΡΠΊΠ°Π½ΠΈ. ΠΠΎΠΌΠΏΠ»Π΅ΠΊΡΠ½ΠΎΠ΅ Π»Π΅ΡΠ΅Π½ΠΈΠ΅ ΠΎΠΊΠ°Π·Π°Π»ΠΎΡΡ Π±ΠΎΠ»Π΅Π΅ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΡΠΌ, ΡΠ΅ΠΌ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ ΠΎΡΠ΄Π΅Π»ΡΠ½ΡΡ
ΠΌΠ΅ΡΠΎΠ΄ΠΈΠΊ.ΠΠΎΠ»ΡΡΠ΅Π½Π½ΡΠ΅ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΡ ΠΎΠ±ΡΡΠ»ΠΎΠ²Π»Π΅Π½Ρ ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½ΡΠ°ΠΌΠΈ ΠΊΡΠ΅ΠΌΠ°, Π²Π»ΠΈΡΡΡΠΈΠΌΠΈ Π½Π° ΠΎΡΠ½ΠΎΠ²Π½ΡΠ΅ ΠΏΡΠΎΡΠ΅ΡΡΡ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΠ·ΠΌΠ° Π² ΠΊΠΎΠΆΠ΅ ΠΈ ΠΏΠΎΠ΄ΠΊΠΎΠΆΠ½ΠΎ-ΠΆΠΈΡΠΎΠ²ΠΎΠΉ ΠΊΠ»Π΅ΡΡΠ°ΡΠΊΠΈ ΠΈ ΠΎΡΠΎΠ±Π΅Π½Π½ΠΎΡΡΡΠΌΠΈ Π΄Π΅ΠΉΡΡΠ²ΠΈΡ ΠΊΡΠΈΠΎΠΌΠ°ΡΡΠ°ΠΆΠ°, Π°ΠΊΡΠΈΠ²Π½ΠΎ Π²Π»ΠΈΡΡΡΠ΅Π³ΠΎ Π½Π° ΡΠΎΡΡΠΎΡΠ½ΠΈΠ΅ ΠΌΠΈΠΊΡΠΎΡΠΈΡΠΊΡΠ»ΡΡΠΈΠΈ
In vitro fertilization of bovine oocytes in protein-free culture system
We studied the possibility of fertilization of bovine oocyte-cumulus complexes, matured in vitro in a protein-free medium, in a protein-free culture system without preliminary capacitation of spermatozoa. The development of embryos to the morula-blastocyst and blastocyst stage was considered as a criterion of successful fertilization. It was shown that replacement of bovine serum albumin for polyvinyl alcohol or polyvinylpyrrolidone in Tyrode medium for fertilization did not affect significantly the development to the morula-blastocyst stage and the number of cells in blastocysts. It was also found that replacement bovine serum albumin for polyvinyl alcohol in all used media, Tyrode medium for washing of oocytes, medium for sperm preparation to fertilization, and Tyrode medium for fertilization, did not affect significantly the development to the morula-blastocyst and blastocyst stages, as well as on the number of cells in blastocysts. The results obtained suggest that in vitro fertilization of bovine oocyte-cumulus complexes is possible in a protein-free culture system without significant reduction in the capacity for in vitro development of the obtained embryos and number of cells in blastocysts