Determination of Lymphocyte Cytokinesis-Block Micronucleus Values in Apparently Healthy Children by means of Age and Sex

COGULU, OZGUR/0000-0002-9037-5599WOS: 000505936600003PubMed: 31950057The cytokinesis-block micronucleus (MN) assay on blood lymphocytes is one of the most important tests implemented in cytogenetics for the measurement of genotoxicity. For the purpose of biological dosing, it is crucial to know the spontaneous frequency of MN and its normal values in general population, especially in children, which are used for the population databases. in this study, MN levels were investigated in cytokinesis-blocked lymphocytes of 150 apparently healthy children aged 1 to 15. Our aim was to assess the variability of MN values according to age and sex. the mean MN frequency among boys was 3.69 +/- 1.747 parts per thousand and 4.12 +/- 1.867 parts per thousand in girls where there was no significant difference in relation to age and sex. However, when we separated age groups as 0-2 years, 3-5 years, 6-10 years, and 11-15 years, one-way ANOVA test showed significant association. Significance was obvious in the 0-2 years age group with the 3-5 years age group and 6-10 years age group. When we grouped our study population as 0-2 years and 3-15 years, the mean MN frequency among the 0-2 years age group was 2.85 +/- 1.599 parts per thousand and 4.07 +/- 1.867 parts per thousand in the 3-15 years age group which was also statistically significant. This difference may be attributed to age-related increase of close contact with environmental hazardous agents. in conclusion, normal values of MN obtained in this study will add valuable information in regard to update the current childhood population data and will act as a reference for further genotoxicity studies.Ege University Scientific Research BoardEge University [13/TIP/0330]This work was supported by the Ege University Scientific Research Board under the number 13/TIP/0330

The relationship between ACTN3 R577X gene polymorphism and physical performance in amateur soccer players and sedentary individuals

WOS: 000452861300002PubMed ID: 30899134The aim of this study was to determine the distribution of ACTN3 R577X gene polymorphism in soccer players and sedentary individuals, and to investigate the relationship of this distribution with performance tests. A total of 100 soccer players and 101 sedentary individuals were enrolled in the study. Standing long jump and countermovement jump (with arm swing, without arm swing and repeated) scores were recorded, using a jump meter. Maximum VO2 levels were measured using a treadmill-connected cardiopulmonary exercise device, Masterscreen CPX. ACTN3 R577X polymorphism was evaluated by real-time PCR. ACTN3 R577X genotype distribution was found to be similar in soccer players and controls (p>0.05). The only statistically significant finding was a shorter countermovement jump with arm swing scores in the RR-genotyped soccer players, compared with their RX genotyped counterparts (p<0.05). In the soccer player group, RX-genotyped subjects were observed to have lower respiratory threshold values compared with RR-genotyped subjects (p<0.05). No significant correlation was detected between this distribution and performance test results. ACTN3 R577X genotype distribution was found to have no effect on sprint and endurance characteristics in amateur soccer players. The ACTN3 R577X polymorphism may not be a specific enough genetic marker to determine athletic performance in soccer