Assessment of Antimicrobial Competence of Epiphytes and Endophytes from Osmium basilicum and Trigonella foenum graecum

Plant-associated bacteria are an unexplored group of microorganisms that has enormous potential. These bacteria are the source of finding new antimicrobial substances. The present study aimed to isolate and characterize the epiphytes and endophytes from Osmium basilicum and Trigonella foenum graecum plants and to determine their antimicrobial potential against pathogenic bacteria from Nishtar Hospital Multan, Pakistan. The sum of 18 stems and roots along leaf specimens were assembled as of the plant\u27s Osmium basilicum plus Trigonella foenum graecum as of the distinct locales of the Multan city. Overall 73 bacterial strains were isolated and their colony morphology, gram staining, spore staining, and characterization were done. Further 10 epiphytic strains and 14 endophytic strains were selected based on their antimicrobial potential intended for a thorough examination. The activity of the selected microbial isolates was determined against pathogenic bacteria K.pneumoniae, MRSA, E.coli, S .aureus, S.typhimurium as of Nishtar hospital. Out of all strains, only one epiphytic and two endophytic bacterial isolates (methanolic extracts) (obr, obl3, and tfen2 were isolated from root, leaf, and root of Osmium basilicum and Trigonella foenum graecum correspondingly inhibited altogether gram-positive besides gram-negative pathogenic microbes involved in this investigation. The hunt to find novel compounds from plant bacteria will open new horizons in the industrial and agricultural sectors

Prevalence and Evaluation of Multidrug Resistance Pattern of Pseudomonas Aeruginosa among Critical and Non-Critical Areas at a Tertiary Care hospital of Multan

Pseudomonas aeruginosa is an extremely wide spread microorganism linked to nosocomial illnesses. Effective inspection of variations in antimicrobial resistance patterns of P. aeruginosa is vital for selecting suitable antimicrobial drugs for pragmatic treatment. The current research has been performed for assessing antimicrobial sensitivity profile of P. aeruginosa isolated as of a variety of medical specimens collected from critical and non-critical admitted patients of Nishtar Hospital, Multan. The isolates were detected utilizing standard lab practices, as well as the sensitivity was examined employing Kirby-Bauer disk diffusion method corresponding to Clinical and Laboratory Standard Institute (CLSI) recommendations 2019. Out of 373 samples, 110 (29.49 %) P. aeruginosa isolates were from admitted patients in different wards. 82 (74.5 %) came from non-critical units along with 28 (25.4 %) belonged to critical units. Prevalence of P. aeruginosafrom the non-critical units was detected from surgical ward 35 (42.6 %) followed by medical ward 25 (30.48 %), gynecology 15 (18.29 %) and orthopedics 7 (8.5 %). The highest prevalence of P. aeruginosa among critical areas were from Medicine Intensive Care Unit 14 (50 %) followed by Surgery Intensive Care Unit 9 (32.14 %) and Respiratory Intensive Care Unit 5 (17.85 %). All were observed as multidrug-resistant against different antibiotics. The current research facilitates estimating the occurrence of MDR strains in intensive care units. Therefore, routine investigation of antibiotic sensitivity patterns is crucial for lowering the healthcare-linked infection levels as well as antimicrobial resistance