32 research outputs found

    Recherche d’un milieu synthĂ©tique pour la croissance et la toxicogenĂšse des Aspergillus du groupe flavus

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    Jacquet Jean, Tantaoui Elaraki Abdelrhani. Recherche d’un milieu synthĂ©tique pour la croissance et la toxicogenĂšse des Aspergillus du groupe flavus. In: Bulletin de l'AcadĂ©mie VĂ©tĂ©rinaire de France tome 128 n°9-10, 1975. pp. 457-466

    Antibacterial effects and toxigenesis of Penicillium aurantiogriseum and P. viridicatum

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    The toxigenesis of one Penicillium aurantiogriseum and one Penicillium viridicatum isolates was investigated. Sterile culture filtrates of both fungi had a clear antibacterial effect only against Bacillus subtilis. The effect on B. subtilis varied with amount of filtrate used and temperature. The antibacterial activity of chloroform extracts varied with the nature of media used to grow the fungi. Different mycotoxins were identified in the fungal cultures using thin-layer chromatography. P. aurantiogriseumwas found to produce penicillic acid, terrestric acid and aurantiamine, while penicillic acid, terrestric acid, brevianamide A and xanthomegnin were produced by P. viridicatum

    Recherche de mycotoxines dans des denrées alimentaires distribuées au Maroc

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    Dans le cadre d'une campagne de contrĂŽle de la contamination des denrĂ©es alimentaires distribuĂ©es au Maroc, 336 Ă©chantillons ont Ă©tĂ© analysĂ©s durant la pĂ©riode 1991-92. Ces Ă©chantillons se rĂ©partissaient en 286 de cĂ©rĂ©ales et son, 44 de raisins secs et 6 d'arachides. L'aflatoxine a Ă©tĂ© recherchĂ©e dans tous les Ă©chantillons, alors que 219 d'entre eux, tous de cĂ©rĂ©ales et son, Ă©taient Ă©galement analysĂ©s pour la recherche de l'ochratoxine A et de la zĂ©aralĂ©none. Un Ă©chantillon de maĂŻs sur 50 analysĂ©s s'est rĂ©vĂ©lĂ© contaminĂ© par l'aflatoxine BI (18 J.1g/kg), et un sur 6 d'arachides Ă©tait fortement polluĂ© (820 J.1g/kg d'aflatoxines BI' B2 , GI et G2). D'autre part, 3 Ă©chantillons d'orge sur 75 contenaient de faibles teneurs en ochratoxine A (1,13 Ă  2,83 J.1g/kg). Ces rĂ©sultats montrent l'innocuitĂ© des cĂ©rĂ©ales distribuĂ©es au Maroc, notamment le blĂ©, ainsi que des raisins secs, en ce qui concerne la contamination par les mycotoxines recherchĂ©es.Mycotoxins survey in foodstuffs distributed in MoroccoAs a part of a broad survey on the contamination of food commodities distributed in Morocco, 336 samples were analysed within 1991-92. These included 286 cereal grains and barn, 44 raisin, and 6 peanut samples. Aflatoxins were looked for in aIl of the salaples, while 219 samples of cereal grains and barn were also investigated for ochratoxin A and zearalenone. One corn sample out of50 was contaminated with aflatoxin BI (18 J.1g/kg), and one of peanuts out of 6 contained 820 J.1g/kg of total aflatoxins BI' B2, GI and G2‱ Also 3 samples ofbarley out 75 of analysed turned out to be contaminated with sm aIl amounts of ochratoxin A (1.13 -2.83 J.1g/kg). These results show the innocuity of cereal grains in Morocco, especially wheat, regarding the contamination with the mycotoxins covered by this study

    The susceptibility of Escherichia coli strains to essential oils of Rormarinus officinalis and Eucalyptus globulus

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    Essential oils are frequently used for flavour and fragrance in the perfume, pharmaceutical, cosmetic and food industries. They are also suitable raw material for production of new synthetic agents. The antibacterial activity of the essential oils obtained by steam distillation of Rosmarinus officinalis L and Eucalyptus globules collected in south Morocco was studied by micro-atmospheric technique against Escherichia coli CIP54127 and E. coli isolated from urine and resistant to several antibiotics. Both essential oils were effective against the two strains bacteria in particularly against E. coli CIP54127. MIQ of both oils ranged from 60 to 70 ìl for both bacteria. In conclusion, results show that essential oils are effective and could be considered as a natural antimicrobial source that can be used on production of natural antimicrobial agents

    Toxicity of south Morocco Rosmarinus officinalis essential oil: antibacterial and histopathological effects

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    Le romarin (Rosmarinus officinalis) possĂšde de nombreuses propriĂ©tĂ©s mĂ©dicinales. Il est connu pour ses effets antimicrobiens et anti-oxydatifs. Le but de ce travail est d’étudier la toxicitĂ© de son huile essentielle (HE) via un test antibactĂ©rien selon la technique de micro-atmosphĂšre et un autre histopathologique au niveau des organes de souris (Swiss albinos). L’analyse de la composition chimique de cette HE a Ă©tĂ© effectuĂ©e par CPG. Les constituants majoritaires de l’HE de R. officinalis sont le 1,8-cinĂ©ole (42%), l’alpha-pinĂšne (11,92%) et le camphre (13.99%). Les quantitĂ©s minimales inhibitrices des souches bactĂ©riennes testĂ©es varient entre 40 ?l et 90 ?l. Au plan histopathologique, une dilatation des alvĂ©oles pulmonaires et une hypertrophie des cellules corticales et mĂ©dullaires des surrĂ©nales ont Ă©tĂ© relevĂ©es. En conclusion, l’HE de R. officinalis ne prĂ©sente pas de toxicitĂ© Ă  la dose 50 ?l/g mais possĂšde des effets stimulants des glandes surrĂ©nales et du systĂšme respiratoire

    Comparison of two methods of chromatography on silica gel for research and dosing of two mycotoxins : A ochratoxin and B 1 and G 1 aflatoxins

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    AprÚs étude des facteurs qui interviennent sur la méthode visuelle utilisant, comme chromatogrammes, des lames pour observations microscopiques, et sur la fluorodensitométrie par réflectance employant un Vitatron TLD 100 et des plaques (20 x 20 cm), nous obtenons, par confrontation des deux techniques une assez bonne concordance entre elles.The authors have studied the factors which behave on visual method, using object-slides, for microscopic examinations, and on fluorodensitometry, employing TLD 100 Vitatron and 20 x 20 cm chromatography plates. They have compared the two technics and concluded that they are in good concordance
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