Enhancement of Closed-State Inactivation and ER Retention of Kv4.3 Mediated by N-Terminal KIS Domain of Auxiliary KChIP4A

BiophysicsSCI(E)CPCI-S(ISTP)0MEETING ABSTRACT3532A-533A10

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

Leptin Regulated ILC2 Cell through the PI3K/AKT Pathway in Allergic Rhinitis

Background. Recent studies suggest that leptin is involved in Th2 response in allergic rhinitis (AR). However, the effect of leptin on type II innate lymphoid cells (ILC2s) in AR is not well characterized. Methods. Twenty-six AR patients and 20 healthy controls were enrolled. Serum leptin levels were measured, and their correlation with ILC2 and type II cytokines were analyzed using enzyme-linked immunosorbent assay (ELISA) and flow cytometry. ILC2 differentiation and cytokine production stimulated by human recombinant leptin were analyzed by real-time polymerase chain reaction (PCR) and ELISA. AR mouse models were also established to verify the effect of leptin on ILC2 cell regulation. Results. Our results showed that elevated serum leptin in AR patients was correlated with the percentage of ILC2 and the expression of type II cytokines. The recombinant leptin enhanced the expression of ILC2 cell transcription factors and type II cytokine through the PI3K/AKT pathway. The AR mice treated with leptin showed as stronger ILC2 inflammation and symptoms compared with control mice. Conclusions. Our data provide evidence that upregulation of leptin promotes ILC2 responses in AR and this process was achieved through the PI3K/AKT pathway

Text Classification Model Enhanced by Unlabeled Data for LaTeX Formula

Generic language models pretrained on large unspecific domains are currently the foundation of NLP. Labeled data are limited in most model training due to the cost of manual annotation, especially in domains including massive Proper Nouns such as mathematics and biology, where it affects the accuracy and robustness of model prediction. However, directly applying a generic language model on a specific domain does not work well. This paper introduces a BERT-based text classification model enhanced by unlabeled data (UL-BERT) in the LaTeX formula domain. A two-stage Pretraining model based on BERT(TP-BERT) is pretrained by unlabeled data in the LaTeX formula domain. A double-prediction pseudo-labeling (DPP) method is introduced to obtain high confidence pseudo-labels for unlabeled data by self-training. Moreover, a multi-rounds teacher–student model training approach is proposed for UL-BERT model training with few labeled data and more unlabeled data with pseudo-labels. Experiments on the classification of the LaTex formula domain show that the classification accuracies have been significantly improved by UL-BERT where the F1 score has been mostly enhanced by 2.76%, and lower resources are needed in model training. It is concluded that our method may be applicable to other specific domains with enormous unlabeled data and limited labelled data

Dynamic Subunit Stoichiometry of Kv4.3-KChIP4A Channel Complexes Visualized by Single-Molecule Subunit Counting

SCI(E)MEETING ABSTRACT2279A-279A10

The Inhibition of Group II Innate Lymphoid Cell Response by IL-27 in Allergic Rhinitis

Objectives. Interleukin-27 (IL-27) has been reported to inhibit type 2 T helper cell (Th2) response in allergic rhinitis (AR). However, its effects on group II innate lymphoid cells (ILC2) in AR are not fully understood. Methods. Nineteen patients with AR and nineteen controls were enrolled in this study. The effects of IL-27 on ILC2 differentiation and function as well as the regulation of the IL-27 receptor (IL-27R) were analyzed by tritiated thymidine incorporation, enzyme-linked immunosorbent assay (ELISA), and real-time polymerase chain reaction (PCR), respectively. AR mice were used to confirm the role of IL-27 in vivo. Results. The serum IL-27 protein expression in AR patients was significantly lower compared with controls. IL-27 decreased the ILC2 proliferation and type II cytokine secretion through the interaction with IL-27R. IL-27 also inhibited systemic and nasal ILC2 response of AR mice. Conclusion. IL-27 inhibited the proliferation and function of ILC2 in AR, implying that IL-27 may be used as new treatment target in AR