The identification and characterization of nucleic acid chaperone activity of human enterovirus 71 nonstructural protein 3AB

AbstractHuman enterovirus 71 (EV71) belongs to the genus Enterovirus in the family Picornaviridae and has been recognized as one of the most important pathogens that cause emerging infectious disease. Despite of the importance of EV71, the nonstructural protein 3AB from this virus is little understood for its function during EV71 replication. Here we expressed EV71 3AB protein as recombinant protein in a eukaryotic expression system and uncovered that this protein possesses a nucleic acid helix-destabilizing and strand annealing acceleration activity in a dose-dependent manner, indicating that EV71 3AB is a nucleic acid chaperone protein. Moreover, we characterized the RNA chaperone activity of EV71 3AB, and revealed that divalent metal ions, such as Mg2+ and Zn2+, were able to inhibit the RNA helix-destabilizing activity of 3AB to different extents. Moreover, we determined that 3B plus the last 7 amino acids at the C-terminal of 3A (termed 3B+7) possess the RNA chaperone activity, and five amino acids, i.e. Lys-80, Phe-82, Phe-85, Tyr-89, and Arg-103, are critical and probably the active sites of 3AB for its RNA chaperone activity. This report reveals that EV71 3AB displays an RNA chaperone activity, adds a new member to the growing list of virus-encoded RNA chaperones, and provides novel knowledge about the virology of EV71

The Path Constitution of Platform Evolution: An Organizational Momentum View

Although the evolutionary pathways are critical to the success of platforms, extant literature has provided little insight into the dynamic processes of the platform evolution, let alone how platforms constitute the evolutionary pathways over time. Against this backdrop, this study examines the evolutionary path constitution of platforms by adopting an organizational momentum lens. Through an exploratory case study of Pinduoduo, it provides evidence of path dependence under the network effects and of path creation resulting from the deliberate actions conducted by the platform firms. Moreover, this study identifies three platform strategies to manage its momentum to facilitate platform evolution and sustainability. Furthermore, this study develops a holistic framework to explain the interactions between platform momentum and path constitution by considering the outcomes of these strategies. The findings and developed framework shed novel light on the path constitution of platform evolution and provide new insight into the sustainability of the platform firms and platform-based markets by leveraging momentum thinking

The Path Constitution of Platform Evolution: An Organizational Momentum View

Although the evolutionary pathways are critical to the success of platforms, extant literature has provided little insight into the dynamic processes of the platform evolution, let alone how platforms constitute the evolutionary pathways over time. Against this backdrop, this study examines the evolutionary path constitution of platforms by adopting an organizational momentum lens. Through an exploratory case study of Pinduoduo, it provides evidence of path dependence under the network effects and of path creation resulting from the deliberate actions conducted by the platform firms. Moreover, this study identifies three platform strategies to manage its momentum to facilitate platform evolution and sustainability. Furthermore, this study develops a holistic framework to explain the interactions between platform momentum and path constitution by considering the outcomes of these strategies. The findings and developed framework shed novel light on the path constitution of platform evolution and provide new insight into the sustainability of the platform firms and platform-based markets by leveraging momentum thinking

Application of NMR and Chemometrics for the Profiling and Classification of Ale and Lager American Craft Beer

In this paper, Nuclear Magnetic Resonance spectroscopy (NMR)-based metabolomics were applied for the discrimination of ale and lager craft American beers. A modified pulse sequence that allows the efficient suppression of the water and ethanol peaks was used to achieve high-quality spectra with minimal sample preparation. The initial chemometrics analysis generated models of low predictive power, indicating the high variability in the groups. Due to this variability, we tested the effect of various data pretreatment and chemometrics approaches to improve the model’s performance. Spectral alignment was found to improve the classification significantly, while the type of normalization also played an important role. NMR combined with statistical and machine-learning techniques such as orthogonal projection to latent structures discriminant analysis (OPLS-DA) and random forest was able to discriminate between ale and lager beers, thus providing an important tool for the quality control and analysis of these products

Expression Characteristics of AaHsp90 Gene in Antheraea assamensis under Different Temperature and Starvation Stress

[Objectives]To investigate the effects of different temperature and starvation stress on the expression of AaHsp90 and reveal the molecular mechanism of adaptation to environment in Antheraea assama.[Methods]Taking the normal feeding group at 26 ℃ as the control,the expression change of AaHsp90 was detected by real-time PCR in midgut,fat body and hemlymph after high temperature stress at 38 ℃,low temperature stress at 4 ℃ and starvation stress separately for different time on the third day of the fifth larvae.[Results]The expression of AaHsp90 in midgut,fat body and hemlymph of Antheraea assama were increased obviously at first and then decreased sharply with the prolongation of treatment time at 38 ℃.There has a certain inhibitory effect on the expression of AaHsp90 in midgut,fat body and hemolymph after treatment with 4 ℃ for different time.After treatment with starvation,the AaHsp90 expression were increased at 12 and 18 h and decreased sharply at 24 h in midgut,fat body and hemolymph of A. assama.[Conclusions]Comprehensive analysis showed that high temperature and starvation stress can induce the expression of AaHsp90,while low temperature stress mainly suppressed its expression.It was suggested that the AaHsp90 protein may play an important role in the process of adaptation to high temperature and starvation stress in A. assama

Using Satellite Remote Sensing to Study the Effect of Sand Excavation on the Suspended Sediment in the Hong Kong-Zhuhai-Macau Bridge Region

The Hong Kong-Zhuhai-Macau Bridge crosses the Pearl River Estuary and is the largest bridge and tunnel project in the world. During the construction period of this project, the excessive suspended sediment was found in the construction region. The suspended sediment generated by sand excavation in the upstream was assumed to have a significant impact on the suspended sediment in the tunnel region. In this study, we assessed the impact of upstream sand excavation on the suspended sediment in the Hong Kong-Zhuhai-Macau Bridge construction area using Landsat OLI, ETM+, and TM data. Regional suspended sediment algorithms were developed for Landsat using a symbolic regression method based on data from in situ measurements in the study area from 2003 to 2014. A band shift was conducted on the remote sensing reflectance data from Landsat ETM+ and OLI to produce a time series of the suspended sediment concentrations that was internally consistent with that of the Landsat TM data. The suspended sediment distribution was extracted and used to compare under two different conditions, with and without sand excavation. The correlations of the time series of the suspended sediment concentrations in different regions in the surrounding waters, including the correlations between the construction regions and the sand excavation regions, were calculated. Our results indicated that the sand excavation north of the Pearl River Estuary had a limited impact on the surface suspended sediment concentrations in the Hong Kong-Zhuhai-Macau Bridge tunnel area

Using Satellite Remote Sensing to Study the Effect of Sand Excavation on the Suspended Sediment in the Hong Kong-Zhuhai-Macau Bridge Region

The Hong Kong-Zhuhai-Macau Bridge crosses the Pearl River Estuary and is the largest bridge and tunnel project in the world. During the construction period of this project, the excessive suspended sediment was found in the construction region. The suspended sediment generated by sand excavation in the upstream was assumed to have a significant impact on the suspended sediment in the tunnel region. In this study, we assessed the impact of upstream sand excavation on the suspended sediment in the Hong Kong-Zhuhai-Macau Bridge construction area using Landsat OLI, ETM+, and TM data. Regional suspended sediment algorithms were developed for Landsat using a symbolic regression method based on data from in situ measurements in the study area from 2003 to 2014. A band shift was conducted on the remote sensing reflectance data from Landsat ETM+ and OLI to produce a time series of the suspended sediment concentrations that was internally consistent with that of the Landsat TM data. The suspended sediment distribution was extracted and used to compare under two different conditions, with and without sand excavation. The correlations of the time series of the suspended sediment concentrations in different regions in the surrounding waters, including the correlations between the construction regions and the sand excavation regions, were calculated. Our results indicated that the sand excavation north of the Pearl River Estuary had a limited impact on the surface suspended sediment concentrations in the Hong Kong-Zhuhai-Macau Bridge tunnel area