RATIO SPECTRA DERIVATIVE UV SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF KETOROLAC TROMETHAMINE AND PHENYLEPHRINE HYDROCHLORIDE IN IMMEDIATE RELEASE TABLET

Objective: To develop and validate robust, accurate and precise UV spectrophotometric method for determination of Ketorolac Tromethamine and Phenylephrine Hydrochloride.Methods: Ratio spectra derivative method was developed using water as solvent. The developed method was validated as per International Conference on Harmonization (ICH) guidelines.Results: Linearity of the developed method was 0.9958 and 0.9987 in the range of 4-20 ppm and 12-60 ppm for Ketorolac Tromethamine and Phenylephrine Hydrochloride respectively. % Relative standard deviation (RSD) was found to be less than 2 for Intraday precision and Intermediate precision. % recovery was found to be 98.5–100.27 % and 98.38–101.99 % for Ketorolac Tromethamine and Phenylephrine Hydrochloride respectively.Conclusion: A robust, accurate and precise UV spectrophotometric method was developed and validated as per ICH guidelines.Â

BIOANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF HCG (HUMAN CHORIONIC GONADOTROPIN)

Objective: To develop and validate simple, rapid, specific, accurate and precise bioanalytical method for determination of the HCG (Human Chorionic Gonadotropin) by RP-HPLC method by using human urine.Methods: The chromatographic separation was performed using Phenom enex C18 (250 x 4.6 mm, 5μ, 300 °A) column. Mobile phase composed of sodium phosphate buffer (pH 7.0, 0.05M) and acetonitrile (87.5:12.5 % v/v) at a flow rate of 1 ml/min. Detection was carried out using UV detector at 215 nm. Bovine serum albumin (BSA) was used as an internal standard (ISTD) and extraction was carried out using protein precipitation method. The method was validated as per USFDA guidelines.Results: The method was linear over the concentration range of 0.37 to 48.4 µg/ml. and correlation coefficient (R2) was found to be 0.9983 and the Lower limit of quantitation (LLOQ) was 0.37 µg/ml. Recovery was found more than 94.0% for HCG. The % CV for interday and intraday precision was found to be less than Ë‚1%.Conclusion: A simple, rapid, specific, accurate and precise analytical method was developed and validated by using human urine.Â

Clinicopathological alterations in naturally occurring Babesia gibsoni infection in dogs of Middle-South Gujarat, India

Aim: The present research work was undertaken to describe various clinical signs and hematobiochemical alterations in dogs affected with Babesia gibsoni. Materials and Methods: Blood smears from a total of 79 suspected dogs of Anand region as well as Surat region of Gujarat state (India) were screened for detection of intraerythrocytic piroplasm of small form of Babesia. Diagnosis was made on the basis of clinical signs and demonstration of B. gibsoni organism in Giemsa-stained thin blood smears. The clinical signs were recorded at the time of presentation, and blood samples were subjected to estimation of hematobiochemical parameters by auto hematology analyzers at College of Veterinary Science and Animal Husbandry, Anand. Statistical analysis, interpretation, and comparison of hematobiochemical changes with scientific literature were carried out to understand the pathophysiology of the disease. Results: Out of 79 dogs, 16 were positive for naturally occurring babesiosis based on the presence of intraerythrocytic piroplasm of small form of Babesia in blood smears. The clinical cases were manifested by wide variety of non-specific clinical signs. The hematological evaluation revealed that the mean values of hemoglobin and total erythrocyte counts in dogs with babesiosis decreased significantly (p<0.01) in comparison to healthy dogs. Among differential leukocyte count, mean values of neutrophils and eosinophils increased while lymphocytes decreased (p<0.01) in dogs with babesiosis in comparison to healthy dogs. Serum biochemistry revealed increase (p<0.01) value of alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, and globulin as well as decrease in albumin levels (p<0.05) in dogs with babesiosis as compared to healthy dogs. Conclusion: B. gibsoni is having multisystemic effects with atypical hematobiochemical changes in dog are discussed here, which would aid new insights in diagnosis of disease