520 research outputs found

    Draft genome sequences of gammaproteobacterial methanotrophs isolated from lake washington sediment.

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    The genomes of Methylosarcina lacus LW14(T) (=ATCC BAA-1047(T) = JCM 13284(T)), Methylobacter sp. strain 21/22, Methylobacter sp. strain 31/32, Methylomonas sp. strain LW13, Methylomonas sp. strain MK1, and Methylomonas sp. strain 11b were sequenced and are reported here. All the strains are obligately methanotrophic bacteria isolated from the sediment of Lake Washington

    Supplier Selection and Development: The Relationship between Small Manufacturing Enterprises and Mass Merchandisers

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    This study examines the results of a program intended to act as a selection tool for mass merchandisers and a development tool for small manufacturers. The evaluation program assessed the management practices and products of potential suppliers. Based on past experience, buyers for mass merchandisers consider small manufacturing enterprises a poor risk as potential suppliers of retail goods. As part of the evaluation process, firms were asked 34 closed-end questions regarding their management practices, and each product was evaluated on 41 specific qualities necessary for the mass merchandising market. Of the 1,690 firms that participated in this project, about 5 percent had their products accepted by a national mass merchandiser. A review of the evaluation data reveals that firms needed high performance in both areas of evaluation to be successful in the marketplace, not just a strong firm or a marketable product. However, each of these areas separately had a statistically significant effect on the success of the product in gaining a retail buyer’s attention

    Nitrogen utilization and metabolic responses of horses to intense anaerobic exercise

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    Call number: LD2668 .T4 ASI 1989 C36Master of ScienceAnimal Sciences and Industr

    The expanded diversity of methylophilaceae from Lake Washington through cultivation and genomic sequencing of novel ecotypes.

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    We describe five novel Methylophilaceae ecotypes from a single ecological niche in Lake Washington, USA, and compare them to three previously described ecotypes, in terms of their phenotype and genome sequence divergence. Two of the ecotypes appear to represent novel genera within the Methylophilaceae. Genome-based metabolic reconstruction highlights metabolic versatility of Methylophilaceae with respect to methylotrophy and nitrogen metabolism, different ecotypes possessing different combinations of primary substrate oxidation systems (MxaFI-type methanol dehydrogenase versus XoxF-type methanol dehydrogenase; methylamine dehydrogenase versus N-methylglutamate pathway) and different potentials for denitrification (assimilatory versus respiratory nitrate reduction). By comparing pairs of closely related genomes, we uncover that site-specific recombination is the main means of genomic evolution and strain divergence, including lateral transfers of genes from both closely- and distantly related taxa. The new ecotypes and the new genomes contribute significantly to our understanding of the extent of genomic and metabolic diversity among organisms of the same family inhabiting the same ecological niche. These organisms also provide novel experimental models for studying the complexity and the function of the microbial communities active in methylotrophy

    Simple Sorting Criteria Help Find the Causal Order in Additive Noise Models

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    Additive Noise Models (ANM) encode a popular functional assumption that enables learning causal structure from observational data. Due to a lack of real-world data meeting the assumptions, synthetic ANM data are often used to evaluate causal discovery algorithms. Reisach et al. (2021) show that, for common simulation parameters, a variable ordering by increasing variance is closely aligned with a causal order and introduce var-sortability to quantify the alignment. Here, we show that not only variance, but also the fraction of a variable's variance explained by all others, as captured by the coefficient of determination R2R^2, tends to increase along the causal order. Simple baseline algorithms can use R2R^2-sortability to match the performance of established methods. Since R2R^2-sortability is invariant under data rescaling, these algorithms perform equally well on standardized or rescaled data, addressing a key limitation of algorithms exploiting var-sortability. We characterize and empirically assess R2R^2-sortability for different simulation parameters. We show that all simulation parameters can affect R2R^2-sortability and must be chosen deliberately to control the difficulty of the causal discovery task and the real-world plausibility of the simulated data. We provide an implementation of the sortability measures and sortability-based algorithms in our library CausalDisco (https://github.com/CausalDisco/CausalDisco).Comment: See https://github.com/CausalDisco/CausalDisco for implementation

    Vermont Price Variation Analysis

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    Presentation to Vermont\u27s Green Mountain Care Board about a Price Variation Analysis undertaken in partnership with the University of Vermont College of Medicine and Wakely Consulting Group. The presentation outlined price variations across the state and suggested a process and methodology that the Board could use to set standard rates

    A strategy to combine pathway-targeted low toxicity drugs in ovarian cancer.

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    Serous Ovarian Cancers (SOC) are frequently resistant to programmed cell death. However, here we describe that these programmed death-resistant cells are nonetheless sensitive to agents that modulate autophagy. Cytotoxicity is not dependent upon apoptosis, necroptosis, or autophagy resolution. A screen of NCBI yielded more than one dozen FDA-approved agents displaying perturbed autophagy in ovarian cancer. The effects were maximized via combinatorial use of the agents that impinged upon distinct points of autophagy regulation. Autophagosome formation correlated with efficacy in vitro and the most cytotoxic two agents gave similar effects to a pentadrug combination that impinged upon five distinct modulators of autophagy. However, in a complex in vivo SOC system, the pentadrug combination outperformed the best two, leaving trace or no disease and with no evidence of systemic toxicity. Targeting the autophagy pathway in a multi-modal fashion might therefore offer a clinical option for treating recalcitrant SOC

    Conflicting Congestion Effects in Resource Allocation Games

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