Screening of Crude Extract of Flavonoids of Moringa oleifera against Bacteria and Fungal Pathogen

Crude extracts were prepared from the flowers and callus of Moringa oleifera using ethanol extract and screened for their antimicrobial activity against some bacteria andfungal pathogen by paper disc method. The tested gram positive bacterial strains were Bacillus subtilis and Staphylococcus aureus, gram negative bacterial strains were Escherichia coli, Klebsiella pneumoniae and fungal pathogen Candida albicans. Although flavonoids are present in all parts of Moringa oleifera, maximum amount was observed in flowers. Hence soxhlet extract of 5 grams of powdered flowers and unorganized tissues of M. oleifera were screened for their antimicrobial activity. Among the flowers and unorganized tissue tested, the ethanol extract of callus exhibited higher antimicrobial activity when compared to the floral extract.Key words: Antimicrobial, Antifungal, Moringa oleifera, Soxhlet extract, Ethanol extractTamanna Talreja. Screening of Crude Extract of Flavonoids of Moringa oleifera against Bacteria and Fungal Pathogen. J Phytol 2/11 (2010) 31-3

PROXIMATE COMPOSITION ANALYSIS OF TWO MEDICINALLY IMPORTANT PLANTS ACHYRANTHES ASPERA AND CISSUS QUADRANGULARIS

Objective: Nutritive status of components of two medicinally important plants Achyranthes aspera (seeds) and Cissus quadrangularis (stem) was determined. These plants have been evaluated for proximate principles to utilize them as a new dietary supplement.Methods: Finely grounded samples of selected plants were subjected to chemical analysis by AOAC (1995). The total nitrogen content was determined following the standard Kjeldahl's method using Kel Plus Semi Automatic Nitrogen Analyzer (Pelican Equipments). Determination of ether extract (EE) was done with the help of Soxhlet's apparatus. For determination of dry matter (DM), crude fiber (CF), nitrogen detergent fibre (NDF), acid detergent fiber (ADF) and total ash (TA), standard conventional procedures were followed. The conventional Weende's method was followed for the calculation of nitrogen free extract (NFE). The method of Talapatra et al. (1940) was followed for the determination of calcium and phosphorus.Results: The CP, CF, TA, NDF, Ca and P of Achyranthes aspera (seeds) and Cissus quadrangularis (stem) respectively were found to be 18.91 and 20.19, 21.03 and 19.19, 2.86 and 1.53, 27.00 and 21.00, 1.98 and 4.28 and 0.49 and 0.47.Conclusion: The evaluation revealed that these plant parts may prove a good dietary supplement for their medicinal use as reported and can compensate the traditional sources which are in a shrinking state.Â

To study the thyroid profile in menstrual disorder at tertiary care hospital in northern western Rajasthan, India

Background: Abnormal uterine bleeding is a common but complicated clinical presentation. It is one of the most frequently encountered conditions in gynaecology and is defined as abnormal bleeding from the uterus.Methods: Present prospective study was conducted on 100 patients who presented with abnormal uterine bleeding at Sardar Patel Medical College, Bikaner, Rajasthan, India.Results: Among 100 women, 39 had hypothyroidism, 8 patients had hyperthyroidism rest 53 were euthyroid. Out of 39 hypothyroid patients, 23 had menorrhagia, 3 had oligomenorrhea and out of 8 patients with hyperthyroidism patients, 6 had oligomenorrhea, 2 had hypomenorrhoea.Conclusions: The menstrual irregularities are significantly more frequent in patients with thyroid dysfunction and menorrhagia was the commonest menstrual abnormality. The study concludes that biochemical evaluation of thyroid function should be made mandatory in all cases of AUB