Grappling with Issues of Learning Science from Everyday Experiences: An Illustrative Case Study

There are different perceptions among researchers with regard to the infusion of everyday experience in the teaching of science: 1) it hinders the learning of science concepts; or, 2) it increases the participation and motivation of students in science learning. This article attempts to contemplate those different perspectives of everyday knowledge in science classrooms by using everyday contexts to teach grade 3 science in Singapore. In this study, two groups of grade 3 students were presented with a scenario that required them to apply the concept of properties of materials to design a shoe. Subsequently, the transcripts of classroom discussions and interactions were analyzed using the framework of sociocultural learning and an interpretative analytic lens. Our analysis suggests that providing an authentic everyday context is insufficient to move young learners of science from their everyday knowledge to scientific knowledge. Further, group interactions among young learners of science to solve an everyday issue need to be scaffolded to ensure meaningful, focused, and sustained learning. Implications for research in science learning among younger students are discussed

Cationic Amino Acid Transporters and Salmonella Typhimurium ArgT Collectively Regulate Arginine Availability towards Intracellular Salmonella Growth

Cationic amino acid transporters (mCAT1 and mCAT2B) regulate the arginine availability in macrophages. How in the infected cell a pathogen can alter the arginine metabolism of the host remains to be understood. We reveal here a novel mechanism by which Salmonella exploit mCAT1 and mCAT2B to acquire host arginine towards its own intracellular growth within antigen presenting cells. We demonstrate that Salmonella infected bone marrow derived macrophages and dendritic cells show enhanced arginine uptake and increased expression of mCAT1 and mCAT2B. We show that the mCAT1 transporter is in close proximity to Salmonella containing vacuole (SCV) specifically by live intracellular Salmonella in order to access the macrophage cytosolic arginine pool. Further, Lysosome associated membrane protein 1, a marker of SCV, also was found to colocalize with mCAT1 in the Salmonella infected cell. The intra vacuolar Salmonella then acquire the host arginine via its own arginine transporter, ArgT for growth. The argT knockout strain was unable to acquire host arginine and was attenuated in growth in both macrophages and in mice model of infection. Together, these data reveal survival strategies by which virulent Salmonella adapt to the harsh conditions prevailing in the infected host cells

Marine extinctions revisited

In recent years, more than 130 extinctions have been estimated to have occurred in the marine realm. Here we review this body of evidence and show that this figure may actually be overestimated by as much as 50%. We argue that previous estimates have not fully taken into account critical uncertainties such as naturally variable geographical distributions, and have misinterpreted documentary evidence. However, current evidence indicates that some sharks, rays and reef-associated species, although not necessarily geographically restricted, are particularly vulnerable to anthropogenic impacts and now occur in very low numbers. Overestimating extinctions is of concern because it could reduce confidence in the credibility of the ‘extinct’ category in threatened species lists and, ultimately, be used to question the integrity of conservation and management policies. We suggest that when integrating future checklists of marine extinct species, there needs to be a more rigorous use of the terminology of extinction, and participation by specialists in each of the particular taxonomic groups involved.Pablo del Monte-Luna, Daniel Lluch-Belda, Elisa Serviere-Zaragoza, Roberto Carmona, Héctor Reyes-Bonilla, David Aurioles-Gamboa, José Luis Castro-Aguirre, Sergio A. Guzmán del Próo, Oscar Trujillo-Millán and Barry W. Broo