ELUTION BEHAVIOR OF DEPHOSPHORAZATION SLAG TO VARIOUS ACIDS AND EFFECT OF ADDITION OF AQUEOUS ALKALI TO ACID ELUATE

We recently developed a method for efficiently recovering phosphoric acid from dephosphorization slag. The most important processes in this recovery technique involve elution of the dephosphorization slag into an acid solution (acid-elution process) and the addition of an alkali to the acid eluate to form a precipitate (alkali-precipitation process). Ultimately, we settled the use of an aqueous nitric acid solution for the acid-elution step, while an aqueous ammonia solution proved to be the optimal choice to accomplish alkali precipitation. Herein, we describe the procedures used to compare the utility of nitric acid with that of hydrochloric acid, sulfuric acid, and citric acid in the acid-elution process, and describe our comparison of an aqueous sodium hydroxide with that of an aqueous ammonia in the alkali-precipitation process. Finally, we summarize our findings on the acids and alkalis that proved to be suitable for this technology

MHD Simulation of The Inner Galaxy with Radiative Cooling and Heating

We investigate the role of magnetic field on the gas dynamics in the Galactic bulge region by three dimensional simulations with radiative cooling and heating. While high-temperature corona with $T>10^6\ {\rm K}$ is formed in the halo regions, the temperature near the Galactic plane is $\lesssim 10^4\ {\rm K}$ following the thermal equilibrium curve determined by the radiative cooling and heating. Although the thermal energy of the interstellar gas is lost by radiative cooling, the saturation level of the magnetic field strength does not significantly depend on the radiative cooling and heating. The magnetic field strength is amplified to $10\ {\rm \mu G}$ on average, and reaches several hundred ${\rm \mu G}$ locally. We find the formation of magnetically dominated regions at mid-latitudes in the case with the radiative cooling and heating, which is not seen in the case without radiative effect. The vertical thickness of the mid-latitude regions is $50-150\ {\rm pc}$ at the radial location of $0.4-0.8 \ {\rm kpc}$ from the Galactic center, which is comparable to the observed vertical distribution of neutral atomic gas. When we take the average of different components of energy density integrated over the Galactic bulge region, the magnetic energy is comparable to the thermal energy. We conclude that the magnetic field plays a substantial role in controlling the dynamical and thermal properties of the Galactic bulge region.Comment: Submitted to ApJ; 21 pages, 18 figures 3 tables. Comment are welcom

The whole blood transcriptional regulation landscape in 465 COVID-19 infected samples from Japan COVID-19 Task Force

「コロナ制圧タスクフォース」COVID-19患者由来の血液細胞における遺伝子発現の網羅的解析 --重症度に応じた遺伝子発現の変化には、ヒトゲノム配列の個人差が影響する--. 京都大学プレスリリース. 2022-08-23.Coronavirus disease 2019 (COVID-19) is a recently-emerged infectious disease that has caused millions of deaths, where comprehensive understanding of disease mechanisms is still unestablished. In particular, studies of gene expression dynamics and regulation landscape in COVID-19 infected individuals are limited. Here, we report on a thorough analysis of whole blood RNA-seq data from 465 genotyped samples from the Japan COVID-19 Task Force, including 359 severe and 106 non-severe COVID-19 cases. We discover 1169 putative causal expression quantitative trait loci (eQTLs) including 34 possible colocalizations with biobank fine-mapping results of hematopoietic traits in a Japanese population, 1549 putative causal splice QTLs (sQTLs; e.g. two independent sQTLs at TOR1AIP1), as well as biologically interpretable trans-eQTL examples (e.g., REST and STING1), all fine-mapped at single variant resolution. We perform differential gene expression analysis to elucidate 198 genes with increased expression in severe COVID-19 cases and enriched for innate immune-related functions. Finally, we evaluate the limited but non-zero effect of COVID-19 phenotype on eQTL discovery, and highlight the presence of COVID-19 severity-interaction eQTLs (ieQTLs; e.g., CLEC4C and MYBL2). Our study provides a comprehensive catalog of whole blood regulatory variants in Japanese, as well as a reference for transcriptional landscapes in response to COVID-19 infection

DOCK2 is involved in the host genetics and biology of severe COVID-19

「コロナ制圧タスクフォース」COVID-19疾患感受性遺伝子DOCK2の重症化機序を解明 --アジア最大のバイオレポジトリーでCOVID-19の治療標的を発見--. 京都大学プレスリリース. 2022-08-10.Identifying the host genetic factors underlying severe COVID-19 is an emerging challenge. Here we conducted a genome-wide association study (GWAS) involving 2, 393 cases of COVID-19 in a cohort of Japanese individuals collected during the initial waves of the pandemic, with 3, 289 unaffected controls. We identified a variant on chromosome 5 at 5q35 (rs60200309-A), close to the dedicator of cytokinesis 2 gene (DOCK2), which was associated with severe COVID-19 in patients less than 65 years of age. This risk allele was prevalent in East Asian individuals but rare in Europeans, highlighting the value of genome-wide association studies in non-European populations. RNA-sequencing analysis of 473 bulk peripheral blood samples identified decreased expression of DOCK2 associated with the risk allele in these younger patients. DOCK2 expression was suppressed in patients with severe cases of COVID-19. Single-cell RNA-sequencing analysis (n = 61 individuals) identified cell-type-specific downregulation of DOCK2 and a COVID-19-specific decreasing effect of the risk allele on DOCK2 expression in non-classical monocytes. Immunohistochemistry of lung specimens from patients with severe COVID-19 pneumonia showed suppressed DOCK2 expression. Moreover, inhibition of DOCK2 function with CPYPP increased the severity of pneumonia in a Syrian hamster model of SARS-CoV-2 infection, characterized by weight loss, lung oedema, enhanced viral loads, impaired macrophage recruitment and dysregulated type I interferon responses. We conclude that DOCK2 has an important role in the host immune response to SARS-CoV-2 infection and the development of severe COVID-19, and could be further explored as a potential biomarker and/or therapeutic target

SFMap: Inferring Services over Encrypted Web Flows Using Dynamical Domain Name Graphs

Part 3: WebInternational audienceMost modern Internet services are carried over the web. A significant amount of web transactions is now encrypted and the transition to encryption has made it difficult for network operators to understand traffic mix. The goal of this study is to enable network operators to infer hostnames within HTTPS traffic because hostname information is useful to understand the breakdown of encrypted web traffic. The proposed approach correlates HTTPS flows and DNS queries/responses. Although this approach may appear trivial, recent deployment and implementation of DNS ecosystems have made it a challenging research problem; i.e., canonical name tricks used by CDNs, the dynamic and diverse nature of DNS TTL settings, and incomplete measurements due to the existence of various caching mechanisms. To tackle these challenges, we introduce domain name graph (DNG), which is a formal expression that characterizes the highly dynamic and diverse nature of DNS mechanisms. Furthermore, we have developed a framework called Service-Flow map (SFMap) that works on top of the DNG. SFMap statistically estimates the hostname of an HTTPS server, given a pair of client and server IP addresses. We evaluate the performance of SFMap through extensive analysis using real packet traces collected from two locations with different scales. We demonstrate that SFMap establishes good estimation accuracies and outperforms a state-of-the-art approach

Day-Night Oscillation of Atrogin1 and Timing-Dependent Preventive Effect of Weight-Bearing on Muscle AtrophyResearch in context

Background: Atrogin1, which is one of the key genes for the promotion of muscle atrophy, exhibits day-night variation. However, its mechanism and the role of its day-night variation are largely unknown in a muscle atrophic context. Methods: The mice were induced a muscle atrophy by hindlimb-unloading (HU). To examine a role of circadian clock, Wild-type (WT) and Clock mutant mice were used. To test the effects of a neuronal effects, an unilateral ablation of sciatic nerve was performed in HU mice. To test a timing-dependent effects of weight-bearing, mice were released from HU for 4 h in a day at early or late active phase (W-EAP and W-LAP groups, respectively). Findings: We found that the day-night oscillation of Atrogin1 expression was not observed in Clock mutant mice or in the sciatic denervated muscle. In addition, the therapeutic effects of weight-bearing were dependent on its timing with a better effect in the early active phase. Interpretation: These findings suggest that the circadian clock controls the day-night oscillation of Atrogin1 expression and the therapeutic effects of weight-bearing are dependent on its timing. Fund: Council for Science, Technology, and Innovation, SIP, “Technologies for creating next-generation agriculture, forestry, and fisheries”. Keywords: Circadian rhythm, Chrono-exercise, Atrogin1, Hindlimb-unloading, Weight-bearin