Structure and Immunosuppresive Activity of Sterols isolated from The Sagami Gulf Hydrozoa Tubularia sp.

Two immunosuppressive sterols, cholesta-5,23E-diene-3β, 25-diol (trans-liagosterol) (A) and cholesta-5,25 (26)-diene-3β, 24ζ-diol (B) were isolated respectively from the Sagami Gulf Hydrozoa Tubularia sp. by the guide of Mitogen bioassey. Structural determination was accomplished mainly by utilizing high-field (400MHz) 2D-NMR experiments, comparing to the spectra of cholesterol. Although these sterols, A and B, were known as the minor components of marine red alga, the presence in marine animal Hydrozoa such as Tubularia sp. was a new discovery. Sterol A inhibited T lymphocyte proliferation which was induced by plant lectins, concanavalin A (Con-A) and phytochemagglutinin (PHA), more than B lymphocyte proliferation which was induced by lipopolysaccharide (LPS). The activity of sterol B was more week than that of sterol A. On the other hand, by the Mitogen bioassay with hydrocortisone (HC) as a standard inhibitor and related five steroids, β-sitosterol (C), stigmesterol acetate (D), resibufogenin (E), 14,21-epoxy-3 β-hydroxy-5 β-chola-20 (21), 22-dienoic acid (F) and ergosterol (G), the inhibition of sterol A for PHA response was found to be most strong in them. However, for LPS response, the inhibition of hydrocortisone (HC) was more powerful than that of sterol A. Also, the immunosuppresive activity of related steroids, C, D, E and G was little found

Significant association of periodontal disease with anti-citrullinated peptide antibody in a Japanese healthy population - The Nagahama study.

歯周病と関節リウマチ発症との相関を示す. 京都大学プレスリリース. 2015-04-21.Anti-citrullinated peptide antibody (ACPA) is a highly specific autoantibody to rheumatoid arthritis (RA). Recent studies have revealed that periodontal disease (PD) is closely associated with RA and production of ACPA in RA. Analyses of associations between PD and ACPA production in a healthy population may deepen our understandings. Here, we analyzed a total of 9554 adult healthy subjects. ACPA and IgM-rheumatoid factor (RF) was quantified and PD status was evaluated using the number of missing teeth (MT), the Community Periodontal Index (CPI) and Loss of Attachment (LA) for these subjects. PD status was analyzed for its association with the positivity and categorical levels of ACPA and RF conditioned for covariates which were shown to be associated with PD, ACPA or RF. As a result, all of MT, CPI and LA showed suggestive or significant associations with positivity (p = 0.024, 0.0042 and 0.037, respectively) and levels of ACPA (p ≤ 0.00031), but none of the PD parameters were associated with those of RF. These association patterns were also observed when we analyzed 6206 non-smokers of the participants. The significant associations between PD parameters and positivity and levels of ACPA in healthy population support the fundamental involvement of PD with ACPA production