Demographic and Clinical Characteristics of Patients with Severe Asthma in the Asian Pacific Region : data from the International Severe Asthma Registry (ISAR)

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Effect of pressure on photochromic furylfulgide

The effect of pressure on photochromic furylfulgide is investigated using monodispersed molecules in solution and molecules in a single crystal by measuring the optical absorption at room temperature under pressure provided using a diamond anvil cell. Monodispersed open-ring (E-form) and closed-ring (C-form) isomers in solution are stable under high pressure; however, pressure-induced E → C isomerisation is observed in a single crystal composed of E-form molecules above 3.0 GPa without UV irradiation. Photochromism of furylfulgide can be observed under high pressures up to 5.0 GPa in both monodispersed molecules and single crystals. In a single crystal, photoinduced conversion from the E-form to the C-form accelerates above a critical value when the fraction of C-form molecules becomes relatively large; this critical value depends on the applied pressure. The acceleration is due to the effect of the surrounding molecules, i.e., destabilisation of E-form molecules surrounded by a sufficient number of photoconverted C-form molecules results in conversion of the E-form molecule without the absorption of a photon

Sequence of the gene for isoleucine tRNA(1) and the surrounding region in a ribosomal RNA operon of Escherichia coli

A DNA fragment of about 2000 base pairs carrying the gene for tRNA(1)(Ile) has been cloned from a total Eco RI endonuclease digest of Escherichia coli DNA. Sequence analyses revealed that about the first 850 base pairs from one end of the fragment contain a nucleotide sequence corresponding to that in the 3'-end of 16S rRNA. The gene for tRNA(Ile) follows the 16S rRNA gene and both genes flank a spacer sequence of 68 base pairs. The spacer region contains a repeating, a hair pin and a symmetrical structure when the sequence is viewed in the single stranded form. A notable hair pin structure is also observed in the region adjacent to the 3'-end of the tRNA(1)(Ile) gene. In addition, about 850 base pairs from the other end of the DNA fragment have been found to contain the nucleotide sequence of the 5'-end of 23S rRNA. The presence of the genes for tRNA(1)(Ile), 16S and 23S rRNA and the hybridization to tRNA(1)(Ala) suggest that this cloned DNA is part of one of the E. coli rRNA operons carrying these two tRNA genes as a spacer