The membrane-embedded segment of cytochrome b5 as studied by cross-linking with photoactivatable phospholipids

Vesicles were prepared from a 9:1 (mole/mol) mixture of dipalmitoyl phosphatidylcholine and the radioactively labeled phospholipids, 1-palmitoyl-2-ω -(m-diazirinophenoxy)undecanoyl-sn-glycero-3-phosphocholine (PC-I) or 1-palmitoyl-2-ω -(2-diazo-3,3,3-trifluropropionyloxy)lauroyl-sn- glycero-3-phosphocholine (PC-II). Rabbit liver cytochrome b5 was inserted into these vesicles spontaneously and the resulting vesicles containing the cytochrome b5 in the transferable form were photolyzed. Cytochrome b5 containing covalently cross-linked phospholipids was isolated by Sephadex LH-60 column chromatography using ethanol/formic acid as the solvent. Of the total radioactivity, 4.6% (PC-I) or 11.3% (PC-II) was linked to the protein; of the former, up to 51% was base-labile, while in the latter, 22% was base-labile. The sites of cross-linking of PC-I to the protein were investigated by fragmentation with trypsin, Staphylococcus aureas V8 protease, CNBr, and o-iodosobenzoic acid followed by Sephadex LH-60 chromatography and Edman sequencing (solid phase) of the appropriate fragments. The distribution of cross-linking was broad (Ser-104 to Met-130), showing a bell-shaped pattern with a significant peak at Ser-118. The labeling pattern is consistent with the previously proposed loop-back model for the membranous segment in the transferable form of cytochrome b5

Solutions to the ultradiscrete Toda molecule equation expressed as minimum weight flows of planar graphs

We define a function by means of the minimum weight flow on a planar graph and prove that this function solves the ultradiscrete Toda molecule equation, its B\"acklund transformation and the two dimensional Toda molecule equation. The method we employ in the proof can be considered as fundamental to the integrability of ultradiscrete soliton equations.Comment: 14 pages, 10 figures Added citations in v

Electron-beam propagation in a two-dimensional electron gas

A quantum mechanical model based on a Green's function approach has been used to calculate the transmission probability of electrons traversing a two-dimensional electron gas injected and detected via mode-selective quantum point contacts. Two-dimensional scattering potentials, back-scattering, and temperature effects were included in order to compare the calculated results with experimentally observed interference patterns. The results yield detailed information about the distribution, size, and the energetic height of the scattering potentials.Comment: 7 pages, 6 figure

Direct Electron-Diffraction Evidence of Charge-Density-Wave Formation in NbSe3

金沢大学理学

Contacts and Edge State Equilibration in the Fractional Quantum Hall Effect

We develop a simple kinetic equation description of edge state dynamics in the fractional quantum Hall effect (FQHE), which allows us to examine in detail equilibration processes between multiple edge modes. As in the integer quantum Hall effect (IQHE), inter-mode equilibration is a prerequisite for quantization of the Hall conductance. Two sources for such equilibration are considered: Edge impurity scattering and equilibration by the electrical contacts. Several specific models for electrical contacts are introduced and analyzed. For FQHE states in which edge channels move in both directions, such as $\nu=2/3$, these models for the electrical contacts {\it do not} equilibrate the edge modes, resulting in a non-quantized Hall conductance, even in a four-terminal measurement. Inclusion of edge-impurity scattering, which {\it directly} transfers charge between channels, is shown to restore the four-terminal quantized conductance. For specific filling factors, notably $\nu =4/5$ and $\nu=4/3$, the equilibration length due to impurity scattering diverges in the zero temperature limit, which should lead to a breakdown of quantization for small samples at low temperatures. Experimental implications are discussed.Comment: 14 pages REVTeX, 6 postscript figures (uuencoded and compressed

Magnetic Quantum Dot: A Magnetic Transmission Barrier and Resonator

We study the ballistic edge-channel transport in quantum wires with a magnetic quantum dot, which is formed by two different magnetic fields B^* and B_0 inside and outside the dot, respectively. We find that the electron states located near the dot and the scattering of edge channels by the dot strongly depend on whether B^* is parallel or antiparallel to B_0. For parallel fields, two-terminal conductance as a function of channel energy is quantized except for resonances, while, for antiparallel fields, it is not quantized and all channels can be completely reflected in some energy ranges. All these features are attributed to the characteristic magnetic confinements caused by nonuniform fields.Comment: 4 pages, 4 figures, to be published in Physical Review Letter

Magnetic Properties of 2-Dimensional Dipolar Squares: Boundary Geometry Dependence

By means of the molecular dynamics simulation on gradual cooling processes, we investigate magnetic properties of classical spin systems only with the magnetic dipole-dipole interaction, which we call dipolar systems. Focusing on their finite-size effect, particularly their boundary geometry dependence, we study two finite dipolar squares cut out from a square lattice with $\Phi=0$ and $\pi/4$, where $\Phi$ is an angle between the direction of the lattice axis and that of the square boundary. Distinctly different results are obtained in the two dipolar squares. In the $\Phi=0$ square, the ``from-edge-to-interior freezing'' of spins is observed. Its ground state has a multi-domain structure whose domains consist of the two among infinitely (continuously) degenerated Luttinger-Tisza (LT) ground-state orders on a bulk square lattice, i.e., the two antiferromagnetically aligned ferromagnetic chains (af-FMC) orders directed in parallel to the two lattice axes. In the $\Phi=\pi/4$ square, on the other hand, the freezing starts from the interior of the square, and its ground state is nearly in a single domain with one of the two af-FMC orders. These geometry effects are argued to originate from the anisotropic nature of the dipole-dipole interaction which depends on the relative direction of sites in a real space of the interacting spins.Comment: 21 pages, 13 figures, submitted to Journal of Physical Society Japa