Fecal carriage and phylodiversity of communityacquired bla TEM Enteric bacilli in Southwest Nigeria

Purpose: Increasing rates of clonal spread of fecal blaTEM bacilli remains a huge concern to the community health with resultant high morbidity. The fecal carriage and clonal diversity of blaTEM within the communities in Southwest Nigeria were surveyed. Materials and methods: Enteric bacilli obtained from fresh fecal samples randomly collected from community residents were biotyped and profiled for antibiotic susceptibility. Resistant strains were typed for beta-lactamase, extended-spectrum beta-lactamases (ESBL), AmpC and carbapenemase production while the R-plasmid carriage was detected and mating activities were examined. The presence of blaTEM gene was assayed by PCR and its phylodiversity determined with 16sRNA genomic profiling. Results: Escherichia coli have the highest (28.6%) occurrence rate and Pseudomonas aeruginosa (20.5%) showing significant resistance to beta-lactamase inhibitors (ampicillin, cefuroxime and cefotaxime), and high-level multidrug resistance of more than 15.2% rate to ampicillin, cefuroxime, ceftazidime, tetracycline and imipenem. E. coli and Klebsiella oxytoca, are the highest beta-lactamase, ESBL and AmpC producers encoded with high molecular weight R-plasmid (>11.0 kbp) and significant rate of conjugation and transformational activities. Only 2/14, 1/13 and 1/6 ESBL-type of E. coli, K. oxytoca and Enterobacter cloaca, expressed blaTEM gene, clustering into five different phylodiverse groups with close genomic relatedness with other bacilli. Conclusion: This is an indication of clonal dissemination of ESBL blaTEM encoded enteric bacilli having high phylodiverse characteristics through fecal carriage in the Nigerian community which requires public health education, food and environmental hygiene for its preventio

Biodegradation of crude petroleum by bacterial consortia from oil-contaminated soils in Ota, Ogun State, South-Western, Nigeria

Pollution from petroleum products is of public health concern because of its attendant health and environmental impacts. Biodegradation of Bonny light crude petroleum and other selected hydrocarbons by bacteria isolated from soils of three different mechanic workshops in Ota, Ogun State were studied. Species of bacteria were isolated by the enrichmentofthesoilsamplesinminimalsaltmediumsupplementedwith1%(v/v)crude petroleumasthesolecarbonandenergysource.Thepetroleumutilizingbacteriabelonging to the genera Bacillus sp. SB4, Pseudomonas sp. SC8, Serratia sp. SC11, and Acinetobacter sp. SC12 were screened and subjected for oil degradation using turbidity and total viable count,reductioninpHandresidualoilconcentrationasindices.GasChromatographic(GC) analysis was used to analyze the component and percentage of the petroleum utilized. Plasmidcuringandprofilingweredonetodeterminewhethertheabilitytoutilizecarbon isplasmidorchromosomallyencoded.Resultsrevealedthatfourbacteriastrainswereable to utilize petroleum as energy source. The GC fingerprints showed that both the aliphatic and aromatic components of crude petroleum were reduced to varying degree but the nonadecane C19 could not be reduced. Strain SC11 could not reduce anthracene, pyrene, benzo(a)pyrene and chrysene components. Strain SB4 depleted 24 to 57% aliphatics and 20to42%aromaticsandstrainSC8depleted38to67%aliphaticsand30to79%aromatics while strain SC11 depleted 12 to 46% aliphatics and 13 to 29% aromatics. All organisms harbored plasmid which could suggest that petroleum degradation capabilities could be plasmid encoded. This indicates that the petroleum utilizing bacteria are could be used bioremediationofthepetroleumpollutedenvironment

Phytochemical Screening and Antimicrobial Studies of Crateva adansonii Leaf Extract

Diverse challenges of microbial infections and upsurge of multi-drug resistant microbes informed the investigation into the phytochemical and antibacterial properties of Crateva adansonii. Cold extraction was carried out using methanol solvent. The crude extract of Crateva adansonii was fractionated into the n-hexane, methanol and chloroform layers successively. The phytochemical screening indicated the presence of alkaloids, saponins, terpenoids, flavonoids and cardiac glycosides. The antimicrobial assay showed that, for Bacillus spp, the organism was sensitive to the chloroform fraction of leaf extract at 1.562 mg/ml. For Microccocus varians, result showed organism was sensitive to the crude extract at 3.125 mg/ml. According to the result of antifungal screening, the n-hexane fraction and crude extract showed activity against Aspergillus niger at 12.500 mg/ml and 3.125 mg/ml respectively. From these results, the crude extract of the leaf of Crateva adansonii shows activity against both bacteria and Fungi; hence, it may might be a good source of new drug for treating infections caused by these pathogen

Momordica charantia stem extract mediated biogenic synthesis of silver nanoparticles: optical and antimicrobial efficacy

In this study, potential of aqueous stem extract of Momordica charantia for phytosynthesis of silver nanoparticles (Ag NPs) was evaluated using reduction method. The locally sourced biodiversity was extracted in water and methanol by cold extraction, after which it was screened for the presence of phytochemicals. Optical measurement was carried out with Uv-visible spectrophotometer, evidence of capping and reduction of Ag+ to Ag° by the biomolecules in the plant extract was achieved using Fourier transformed infra-red (FT-IR). Antimicrobial activity of the green synthesized nanoparticles was tested on clinical isolates. Visual evidence of Ag NPs formation was detected by change in reaction solution colour from light brown to deep brown, which resulted in excitation of surface plasmon resonance (SPR) between 400- 450 nm. Growths of microbes used were significantly inhibited (P < 0.05) with increasing concentrations of Ag NPS. Moderate activity was displayed by Ag NPs with the same value of MIC and MBC (12.5 mg/mL) on all the organisms considered except P. aeruginosa in which low activity was observed. The enhanced SPR displayed by Ag NPs qualifies them as potential materials for therapeutic and diagnostic uses

Sustainable nano-sodium silicate and silver nitrate impregnated locally made ceramic filters for point-of-use water treatments in sub-Sahara African households

The poor access to water quality for Nigerians has pushed for the designing of new trend silver nitrate impregnated locally made Point-Of-Use (POU) ceramic filters to enhance water purification efficiency for household use. This study utilized silver nitrate-molded ceramic filters prepared with Kaolin from Owode, silt soil, sodium silicate, sawdust, and distilled water in three varying proportions to ascertain pollution removal efficiencies. Heating was carried out by firing the filters at 900 �C and further preheating at 400 �C after dipping in silver nitrate solution. Silver nanoparticle and dissociated particle discharge from filter pot painted with 0.03 mg/g caseincovered nAg or AgNO3 were estimated as an element of pH (5–9), ionic strength (1–50mM), and cation species (Naþ, Ca2þ, Mg2þ). Silver delivery was constrained by disintegration as Agþ and resulting cation exchange measures, paying little heed to silver structure applied. Water analysis for both heavy metals (Pb and Cd) and microbial load (E. coli) evaluated, corroborate the maximum removal efficiency. It was observed that kaolinsawdust with the Silver nitrate filters showed a constant and effective removal of both heavy metals and disinfection of microbial loads. The minimum flow rates observed were 4.97 mL/min for batch filter used for Iju River water sample one (AF1) and 4.98 mL/min for batch filter used for Iju River water sample two (AF2) having porosity 49.05% and 50.00%, whereas the 5 mL/min higher flow rate was used for batch filter from borehole water sample one (BF1) and batch filter used for well water sample two (CF2) with porosity of 50.00%. Significantly, the results obtained show that the filters are suitable for point-of-use application in both the urban and rural areas of developing countries such as Nigeri

Chapter 21 - Utilization of nanochitosan in the sterilization of ponds and water treatment for aquaculture

Water pollution constitutes the leading cause of infant mortality, neonatal deformities, and shrinkage of man’s average life expectancy. Pollutants come from point and nonpoint sources; and water pollution arises from the discharge of wastewater containing undesirable impurities used for domestic, agricultural, and industrial purposes. More so, high nutrient and wastewater runoffs from fish production systems contribute to the fouling and eutrophication of recipient water bodies. Hence, aquaculture which is inextricably linked to the natural environment is challenged by the dearth of appropriate water quantity and quality, militating against fish, and fishery production. Nanochitosans as polysaccharides produced by the alkalescent deacetylation of chitin, comprise a series of 2-deoxy-2 (acetylamino) glucose linked by ß-(1-4) glycosidic linkages. They are naturally formed from the deacetylation of shellfish shells and exoskeletons of aquatic arthropods and crustaceans. The unique attributes of chitin confer a wide range of biotechnological applications on the polymer, observed in flocculation as a wastewater treatment and purification route initiated by chitosan. This chapter highlights nanochitosan properties of aquaculture relevance; and elucidates the purification potentials of nanochitosan, compared to inorganic coagulants and organic polymeric flocculants. Effects of chitosan on contaminants and microorganisms, as well as applications in fish pathogens detection, fish disease diagnosis, and control are discussed

A Bioremediation Study of Raw and Treated Crude Petroleum Oil Polluted Soil with Aspergillus niger and Pseudomonas aeruginosa

This study was conducted to investigate the degree of bioremediation that would occur in the samples of soil polluted with raw and treated crude petroleum (oil) with the aid of Aspergillus niger (fungi) and Pseudomonas aeruginosa (bacteria). This was achieved by monitoring the Organic carbon content in the soil over 45 days and pH over 25 days. Four systems of 500 g soil were polluted with 40 g treated crude petroleum, while four systems were contaminated with 40 g raw crude petroleum. The eight systems were labeled accordingly. Two systems for raw crude control and treated crude control (RCC and TCC) were left as control, two systems for raw crude Aspergillus niger and treated crude Aspergillus niger (RCA and TCA) were treated with Aspergillus niger only, two systems for raw crude Pseudomonas aeruginosa and treated crude Pseudomonas aeruginosa (RCP and TCP) were treated with Pseudomonas aeruginosa only and the last two systems for raw crude Aspergillus niger and Pseudomonas aeruginosa (RCAP and TCAP) were treated with both Pseudomonas aeruginosa and Aspergillus niger . At the end of the bioremediation period, the results obtained showed that the pH was not particularly a solid parameter to estimate the degree of bioremediation. This is because a proper trend in the results obtained could not be determined. Additionally, it was observed that Aspergillus niger (fungi) and Pseudomonas aeruginosa (bacteria) alone and separate remediate raw crude polluted soil better than treated crude polluted soil. This was determined by the TOC (Total Organic Carbon) values on the 45th day of the experiment

Isolation of Polygalacturonase-Producing Bacterial Strain from Tomatoes (Lycopersicon esculentum Mill.)

Background. Polygalacturonase (EC 3.2.1.15) enzyme aids in microbial spoilage of fruits and vegetables. It is very important to find economical ways to producing the enzyme so as to achieve maximum yield in industries due to its use at different areas of production process. Methods. Isolation of polygalacturonase-producing bacterial strain from tomatoes (Lycopersicon esculentum Mill.) was studied. Polygalacturonase-producing bacterial strains were isolated and screened from tomatoes stored at normal laboratory temperature (25 ± 2°C). They were identified based on their morphological, biochemical, and molecular characteristics. The enzyme produced was partially purified by the ammonium sulphate precipitation method. Molecular weights and optimum conditions for best enzyme activity were obtained by SDS PAGE technique. Results. Five bacterial isolates resulted after screening. Bacterial strain code B5 showed highest polygalacturonase activity. Optimum conditions for polygalacturonase PEC B5 were maintained at pH 4.5; temperature 35°C; substrate concentration 0.3 mg/ml, and best activity at less than 5 min of heating. The enzyme PEC B5 was found to weigh 65 kDa and 50 kDa for crude and partially purified aliquots, respectively. The result of 16S rRNA gene sequencing revealed bacterial strain code B5 as Enterobacter tabaci NR146667 having 79% similarity with the NCBI GenBank. Conclusion. Microorganisms should be developed for large-scale production of enzymes in developing countries

Comparison of Splatter and Aerosol Mitigation by two Suction Devices

Aim or Purpose: Previous studies suggested that size and shape of evacuators were important considerations to ensure aerosol and splatter reduction when using rotary instruments in dentistry, therefore this study aims to compare the aerosol and splatter mitigation by 2 suction methods in a simulated setup. Materials and Methods: Study approval was obtained from the Lagos State University Teaching Hospital Ethics Committee. In an enclosed operatory, a funnel shaped prototype 3D-printed (PROTO) evacuator tip was compared with a conventional high volume evacuator (HVE) tip in mitigating splatter and aerosol. An all-ceramic crown preparation was prepared on tooth number 46 using a diamond bur with a high-speed air-turbine for 60s, and repeated six times for each suction setup to simulate a patient under care. Quantity of splatter and aerosol generated during tooth preparation procedures with no suction as well as when both suction devices were used were measured. Comparison was by two-way analysis of variance and posthoc pairwise comparisons using a Tukey adjustment. Results: The PROTO method had statistically significant less environmental splatter (P < 0.014) and aerosol (P = 0.007) than the conventional HVE device. Both PROTO and HVE significantly reduced splatter compared to the control with no suction, however, no significant difference was observed in the amount of aerosol when compared with the control trials. Conclusions: These results showed that both devices mitigated splatter better than aerosol. It therefore provide evidence for adjunctive means of reducing risk of direct transmission of infection in the dental practice by combining strategies of protective procedures, improved room ventilation and use of air filtration system