Natural Variation in the Flag Leaf Morphology of Rice Due to a Mutation of the NARROW LEAF 1 Gene in Oryza sativa L.

We investigated the natural variations in the flag leaf morphology of rice. We conducted a principal component analysis based on nine flag leaf morphology traits using 103 accessions from the National Institute of Agrobiological Sciences Core Collection. The first component explained 39% of total variance, and the variable with highest loading was the width of the flag leaf (WFL). A genome-wide association analysis of 102 diverse Japanese accessions revealed that marker RM6992 on chromosome 4 was highly associated with WFL. In analyses of progenies derived from a cross between Takanari and Akenohoshi, the most significant quantitative trait locus (QTL) for WFL was in a 10.3-kb region containing the NARROW LEAF 1 (NAL1) gene, located 0.4 Mb downstream of RM6992. Analyses of chromosomal segment substitution lines indicated that a mutation (G1509A single-nucleotide mutation, causing an R233H amino acid substitution in NAL1) was present at the QTL. This explained 13 and 20% of total variability in WFL and the distance between small vascular bundles, respectively. The mutation apparently occurred during rice domestication and spread into japonica, tropical japonica, and indica subgroups. Notably, one accession, Phulba, had a NAL1 allele encoding only the N-terminal, or one-fourth, of the wild-type peptide. Given that the Phulba allele and the histidine-type allele showed essentially the same phenotype, the histidine-type allele was regarded as malfunctional. The phenotypes of transgenic plants varied depending on the ratio of histidine-type alleles to arginine-type alleles, raising the possibility that H(233)-type products function differently from and compete with R(233)-type products

UTILIZATION OF DNA MARKERS FOR EVALUATING AMYLOSE CONTENT OF GRAIN IN RICE

The sensory and functional properties of rice are predominantly associated with its amylose content. Granule-bound starch synthase (GBSS) encoded by the Waxy (Wx) gene determines the synthesis of amylose, while starch branching enzymes encoded by Sbe genes are involved in the formation of amylopectin. Some studies have demonstrated that Wx gene is the major controller of amylose content but there are one or more modifying genes affecting the amylose content. Three markers,  microsatellite, Single – nucleotide – polymorphism (G/T SNP) in Wx gene and Single – nucleotide – polymorphism (T/C SNP) in Sbe1 gene, were tested for their association with amylose content using sixty-nine  rice accessions from twenty countries. Of the three markers, two markers in Wx gene are significantly associated with amylose content. The combination of two markers in Wx gene (haplotypes) explained 83.8% of the variation in amylose content and discriminated the three market classes of glutinous, low, intermediate and high amylose content of rice from each other. And T/C SNP in Sbe1 locus was not a suitable marker for amylose content. Keywords: marker, amylose content, Waxy gene

UTILIZATION OF DNA MARKERS FOR EVALUATING AMYLOSE CONTENT OF GRAIN IN RICE

The sensory and functional properties of rice are predominantly associated with its amylose content. Granule-bound starch synthase (GBSS) encoded by the Waxy (Wx) gene determines the synthesis of amylose, while starch branching enzymes encoded by Sbe genes are involved in the formation of amylopectin. Some studies have demonstrated that Wx gene is the major controller of amylose content but there are one or more modifying genes affecting the amylose content. Three markers,  microsatellite, Single – nucleotide – polymorphism (G/T SNP) in Wx gene and Single – nucleotide – polymorphism (T/C SNP) in Sbe1 gene, were tested for their association with amylose content using sixty-nine  rice accessions from twenty countries. Of the three markers, two markers in Wx gene are significantly associated with amylose content. The combination of two markers in Wx gene (haplotypes) explained 83.8% of the variation in amylose content and discriminated the three market classes of glutinous, low, intermediate and high amylose content of rice from each other. And T/C SNP in Sbe1 locus was not a suitable marker for amylose content. Keywords: marker, amylose content, Waxy gene

Effects of a Reduction in Soil Moisture from One Month beforeFlowering through Ripening on Dry Matter Production and Ecophysiological Characteristics of Wheat Plants

We found in the previous study that the wheat plants grown under relatively low soil moisture conditions (D plot) could attain heavier dry matter than the plants watered on the basis of average local precipitation (W plot). The aim of this study was to make a detail analysis of the ecophysiological characteristics that cause the difference in dry matter production between the plants in the W and D plots under different soil moisture conditions. Soil matric potential at a depth of 30 cm was kept at about −4 kPa in the W plot. It decreased gradually after watering at about one month before heading and at heading, reaching about −80 kPa at heading stage and at the mid-ripening stage respectively, in the D plot. The plants in the D plot produced heavier dry matter and a better developed root system than the plants in the W plot. The higher net assimilation rate and larger leaf area, which accounted for the higher crop growth rate of the D plot, weredue both to avoiding suppression of the photosynthetic rate and leaf expansion owing to water stress, and to maintaining high rates of leaf photosynthesis and a large leaf area during leaf senescence. A larger amount of nitrogen was accumulatedat the flowering stage and the nitrogen content of leaves remained higher during senescence in plants in the D plot than those in the W plot. The activity of cytokinins in the xylem sap was higher in plants in the D plot. These characteristics might have contributed to the delay in the decline in the rate of photosynthesis and in leaf area during leaf senescence and seemed to be supported by the enhanced development of the root system under moisture-restricted conditions

Interaction of Scion and Stock on Leaf Senescence of Soybean Plants Grafted at Mid-Stem during Ripening

Leaf senescence is slower in the soybean cultivar Tachinagaha (T) than in the cultivar Enrei (E). Reciprocal grafting of the two cultivars at the basal node showed that this difference was related to roots properties. However, roots had no effect on leaf senescence at a late stage of ripening. To investigate whether the properties of the above-ground parts of plants affect leaf senescence, we grafted the two cultivars at the internode between the 8th and 9th nodes of the stem. Regarding the effect of the scion on the stock, the chlorophyll content of leaves on the E stock was maintained at a higher level when the scion was T than it was E, and the chlorophyll content of leaves on the T stock decreased faster when the scion was E than when it was T. Regarding the effect of the stock on the scion, the chlorophyll content of leaves on the T scion decreased faster when the stock was E than when it was T, although the effect of the stock on leaf senescence of the scion were weaker compared with that of the scion on the stock. Differences in photosynthetic rate were similar to those in chlorophyll content. Thus, it was clear that leaf senescence was affected by the properties of both the higher and lower above-ground parts of plants, in addition to roots

Varietal Differences in Photosynthetic Rates in Rice Plants, with Special Reference to the Nitrogen Content of Leaves

The photosynthetic rate in the fl ag leaf of rice at the full heading stage was examined in three japonica varieties, Koshihikari, Aikoku and Asanohikari, and the indica high-yielding variety Takanari at the same level of leaf nitrogen. At an ambient CO2 concentration of 350 µL L-1, Takanari had a higher photosynthetic rate and stomatal conductance than the japonica varieties when plants were compared at a leaf nitrogen content of approximately 1.5 g m-2. Stomatal conductance increased considerably with increases in leaf nitrogen content in the japonica varieties. As a result, at a leaf nitrogen content of approximately 2.0 g m-2, differences in terms of the photosynthetic rate among varieties were small. By contrast, there were no clear varietal differences in Rubisco content at any identical nitrogen content of leaves. We conclude that stomatal conductance is responsible for the varietal differences in photosynthetic rate examined at the same leaf nitrogen content