Antibody formation for malignant tumor. II. Antigenicity of Ehrlich ascites tumor lipoprotein

A unique low density lipoprotein was obtained from the tumor transplanted with a cultured cell line of Ehrlich ascites tumor, JTC-ll cell. The tumor low density lipoprotein electrophoretically migrated as a single band, and the mobility was different from that of other organs. The chemical composition of lipid, cholesterol and phospholipids in tumor low density lipoprotein were characteristic. The flotation rate was Sf 5.9, and thus the molecular weight was estimated to be about 130 x 104. The inhibitory effect on tumor growth of the immune serum was most elevated at 25th day after the intraperitoneal administration of tumor low density lipoprotein. The main fraction effective for inhibition of tumor growth existed in &#947;-globulin.</p

Antigenicity of Ehrlich Ascites Tumor Part 2, Antigenicity of Ribosomal Protein in Ehrlich Ascites Tumor

Antigenicity of ribosomal protein in Ehrlich ascites tumor and the biochemical characters were studied. The cytotoxic activity of immunized male dd-mice serum globulin was proved by cell culture test. Ribosomal protein was moved electrophoretically to basic side than that of serum γ-globulin, and the protein concentraticn was adequate for maximal antigenicity 1 mg per mouse. The antitumor activity of immunized εerum globulin was most elevated at 25 th day after immunization, and that of lymph nodes and spleen were at 10 th day. The antitumor activity of immunized serum globulin was mainly existed in γ-globulin fraction, but also contained in α-and β-globulin fraction

Antigenicity of Ehrlich Ascites Tumor Part 1, Antigenicity of Low Density Lipoprotein in Ehrlich Ascites Tumor

Antigenicity of insoluble lipoprotein (low density lipoprotein) in Ehrlich ascites tumor and its biochemical characters were studied. The cytotoxic effect of immunized male dd-mice serum globulin was examined by cell culture technique. The insoluble lipoprotein used as antigen was moved electrophoretically similar with serum β-lipoprotein, and the maximal antigenicity was obtained by administration of 1 mg protein concentration per mouse as antigen. The cytotoxic effect was mainly existedin γ-globulin fraction. The antitumor activity was most elevated at 25 th day after immunization with low density lipoprotein, and the effect was decreased gradually after 30 th day. The globulin fraction in lymph nodes and spleen was showed the highest cytotoxic activity at 10 th day after immunization