3 research outputs found
Activity and stability of catalase in the organs of acatalasemic mice-Comparison of activities at different incubating temperatures by the perborate method
Catalase activities in the organs of normal, acatalasemic and heterozygous-hypocatalasemic mice were determined after the tissues were incubated with perborateat different temperatures. Catalase activity in the normal mouse hemolysate and liver homogenate measured after being incubated with perborate at 20β for 5 min. was slightly lower than that measured at 37β. In contrast, residual catalase activity in acatalasemic mouse hemolysate and liver homogenate measured at 20β for 5 min. was markedly higher than that measured at 37β, suggesting that residual catalase in the acatalasemic mouse blood and liver is inactivated during incubation with perborate at 37β for 5 min. Catalase activity in the blood and solid tissues of acatalasemic mice measured at 20β was 1.29 to 3.30 times as high as that measured at 37β. The ratio of catalase activity in the blood and solid tissues of acatalasemic mice to that in normal mouse tissues incubated with perborate at 20β for 5 min. was calculated to be 4.3% in blood, 65.7% in liver, 15.6% in kidneys, 69.9% in lungs, 33.7% in stomach, 30.0% in brain and 31.8% in heart. Catalase activity in the blood and solid tissues in heterozygous hypocatalasemic mice measured at 20β was 1.09 to 1.70 times as high as that measured at 37β. This ratio of catalase activity in the heterozygous hypocatalasemic mouse tissue incubated at 20β to the activity of tissue incubated at 37β is between the ratio of normal mice and that of acatalasemic mice. The ratio of catalase activity in the blood and solid tissues of heterozygous hypocatalasemic mice to the activity in the corresponding organs of normal mice measured at 20β for 5 min. was calculated to be 47.3% in blood, 95.0% in liver, 36.6% in kidneys, and 44.9% in stomach
Activity and stability of catalase in the organs of acatalasemic mice-Comparison of activities at different incubating temperatures by the perborate method
Catalase activities in the organs of normal, acatalasemic and heterozygous-hypocatalasemic mice were determined after the tissues were incubated with perborateat different temperatures. Catalase activity in the normal mouse hemolysate and liver homogenate measured after being incubated with perborate at 20β for 5 min. was slightly lower than that measured at 37β. In contrast, residual catalase activity in acatalasemic mouse hemolysate and liver homogenate measured at 20β for 5 min. was markedly higher than that measured at 37β, suggesting that residual catalase in the acatalasemic mouse blood and liver is inactivated during incubation with perborate at 37β for 5 min. Catalase activity in the blood and solid tissues of acatalasemic mice measured at 20β was 1.29 to 3.30 times as high as that measured at 37β. The ratio of catalase activity in the blood and solid tissues of acatalasemic mice to that in normal mouse tissues incubated with perborate at 20β for 5 min. was calculated to be 4.3% in blood, 65.7% in liver, 15.6% in kidneys, 69.9% in lungs, 33.7% in stomach, 30.0% in brain and 31.8% in heart. Catalase activity in the blood and solid tissues in heterozygous hypocatalasemic mice measured at 20β was 1.09 to 1.70 times as high as that measured at 37β. This ratio of catalase activity in the heterozygous hypocatalasemic mouse tissue incubated at 20β to the activity of tissue incubated at 37β is between the ratio of normal mice and that of acatalasemic mice. The ratio of catalase activity in the blood and solid tissues of heterozygous hypocatalasemic mice to the activity in the corresponding organs of normal mice measured at 20β for 5 min. was calculated to be 47.3% in blood, 95.0% in liver, 36.6% in kidneys, and 44.9% in stomach