Anterior Z250 resin composite restorations: one-year evaluation of clinical performance.

AbstractThe purpose of this practice-based study was to compare the clinical performance of a new universal composite resin material (Z250) used for Class III and V cavities in anterior teeth. Restorative materials (Z250 and Z100; 3 M ESPE) placed by six operators were used for a total of 150 restorations during the 6-month enrollment period. After 1 year, 141 restorations (76 Z250 and 66 Z100) were available and evaluated for overall quality, color match, marginal adaptation, surface appearance and the presence of secondary caries, using modified USPHS criteria. The overall quality was excellent for both materials and no significant changes were noted during the follow-up. None of the scores between the two materials were statistically significant. Major changes were seen in color match and surface appearance. At baseline, the color match of 71% of Z250 and 62% of Z100 was rated as Alfa, after 1 year the figures were 60 and 65%. Regarding surface appearance, 97% of the Z250 were rated Alfa at baseline, whereas at 1 year the figure was 76%. For Z100, the scores were 94 and 79%, respectively. After 1 year, the clinical performance of Z250 restorative composite resin was clinically acceptable and similar to that of Z100.</div

Effect of ultraviolet light treatment on surface hydrophilicity and human gingival fibroblast response on nanostructured titanium surfaces

This study was designed to investigate the effect of nanostructured TiO2 coatings on human gingival fibroblast and to explore the influence of ultraviolet (UV) light on surface wettability and cellular response. Ti-6Al-4V titanium alloy discs (n=96) were divided into three groups: a sol-gel-derived MetAlive (MA) coating; hydrothermal (HT) coating; and a non-coated (NC) group. Forty-eight titanium substrates were further treated with UV light for 15min. The water contact angles of the substrates were measured using the sessile drop method. Human gingival fibroblasts were used to evaluate the cell adhesion strength and cell proliferation on experimental surfaces. The strength of cell adhesion against enzymatic detachment was studied after 6hr of adhesion using gentle trypsinization for 15min at room temperature. A fluorescence microscope was used for cell imaging (Zeiss-stereo-lumar-v12), and images were analyzed for cell counting, and the percentage of detached cells were calculated. The proliferation of cultured cells up to 10days was determined according to the cell activity using Alamar Blueassay. The HT group had the lowest contact angle value (31.1 degrees) followed by MetAlive (35.3 degrees), whereas the NC group had the highest contact angle (50.3 degrees). After UV light treatment, all surfaces become considerably more hydrophilic. There was a significant difference in the amount of adherent cells between sol-gel and HT groups when compared with the NC group (p<.05) with detachment percentages of 35.8%, 36.4%, and 70.7%, respectively. All substrate types showed an increase in cell proliferation rate until 10days. It can be concluded that nanostructured titanium oxide implant surfaces, obtained by sol-gel and HT coating methods, enhance the surface wettability and improve human gingival fibroblast function in terms of adhesion and proliferation rate when compared with non-coated surfaces. UV light treatment clearly enhances the wettability of all titanium surfaces