Characterization of GATA/GACA-related sequences on proximal chromosome 17 of the mouse

Autosomal loci have long been thouht to have a role in sex determination of mice. We studied the localization of GATA/GACA repeats on chromosome 17 in regard to the possibility of their involvement in sex determination. We performed in situ hybridizations on chromosome 17s carrying the Hairpain tail ( T hp ) deletion of the T locus since this deletion has been associated with sex reversal and hermaphroditism. We did not detect a significant decrease in the amount of hybridization of GATA/GACA repeats to the T hp deletion. In addition, three Bkm-positive cosmids from proximal chromosome 17 did not contain sequences deleted in T hp or T orl and a fetal testes cDNA probe did not hybridize to the cosmid sequences. Although we confirmed the localization of Bkm-related sequences on chromosome 17, we were not able to relate GATA/GACA sequences on chromosome 17 to sex determination in mice.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/47363/1/412_2004_Article_BF00371970.pd