Not Available

Not AvailableThe genetic diversity among 24 Flue-cured Virginia (FCV) tobacco cultivars was investigated using 20 SSR primers. Distinct polymorphism was noticed among closely related cultivars studied indicating the robust nature of microsatellites in revealing polymorphism. A total of 78 amplicons were produced of which 48% were polymorphic among the varieties. The pairwise similarity among the varieties ranged from 0.54 to 0.91, indicated a broad genetic base of the tested genotypes which could be due to use of divergent parents including exotic introductions and local selections in breeding for different quality and physiological traits. Varieties developed indigenously through different breeding methods were clustered together whereas exotic introductions and their derivatives were grouped together separately. Genetic relationship among the FCV tobacco varieties based on polymorphic DNA profile largely corresponded with parentage. The SSR based analysis in the present study suggested that the Indian tobacco breeding has been successful in developing varieties with a relative broader genetic base.Not Availabl

Not Available

Not AvailableIn the present study, an effort was made for the development of mapping population for mapping of Tobacco-specific nitrosamines (TSNA) in tobacco. TSNAs are found only in tobacco products, and are considered to be harmful to tobacco consumers. Molecular mapping of TSNA trait aid in tailoring the tobacco plants to yield lower levels of TSNAs using marker assisted breeding strategies. In order to select the parents for developing mapping populations, 11 burley lines were characterized for their TSNA yielding abilities and molecular polymorphism using 34 RAPD markers. Based on this, three polymorphic lines viz., Banket-A1, By-64 and VA-510 were identified as parents for developing mapping population. Banket-A1 is a high TSNA yielding variety and selected as one of the parent. VA-510, a low TSNA line recording higher molecular diversity with Banket-A1 was crossed with Banket-A1 for developing mapping population. Another line, By-64 recording low TSNA, having desirable morphological characters and least molecular diversity with Banket-A1 was crossed with Banket-A1 for developing another mapping population. Recombinant Inbred Lines (RILs) were developed from both the crosses through single seed descent method. These populations developed using diverse parents are of immense use in mapping of genes governing TSNA content in tobacco.Not Availabl

Not Available

Not AvailableNot AvailableNot Availabl

Not Available

Not AvailableNot AvailableNot Availabl

Not Available

Not AvailableIn the present study, an effort was made for the development of mapping population for mapping of Tobacco-specific nitrosamines (TSNA) in tobacco. TSNAs are found only in tobacco products, and are considered to be harmful to tobacco consumers. Molecular mapping of TSNA trait aid in tailoring the tobacco plants to yield lower levels of TSNAs using marker assisted breeding strategies. In order to select the parents for developing mapping populations, 11 burley lines were characterized for their TSNA yielding abilities and molecular polymorphism using 34 RAPD markers. Based on this, three polymorphic lines viz., Banket-A1, By-64 and VA-510 were identified as parents for developing mapping population. Banket-A1 is a high TSNA yielding variety and selected as one of the parent. VA-510, a low TSNA line recording higher molecular diversity with Banket-A1 was crossed with Banket-A1 for developing mapping population. Another line, By-64 recording low TSNA, having desirable morphological characters and least molecular diversity with Banket-A1 was crossed with Banket-A1 for developing another mapping population. Recombinant Inbred Lines (RILs) were developed from both the crosses through single seed descent method. These populations developed using diverse parents are of immense use in mapping of genes governing TSNA content in tobacco.Not Availabl