Serum metabolome changes in adult patients with severe dengue in the critical and recovery phases of dengue infection

10.1371/journal.pntd.0006217PLoS Neglected Tropical Diseases121e000621

Basic or applied, it's the interaction that counts - Toxicology must not be driven by politics: it's science that should drive decision-making

Correspondence - no abstract available

Anti-malarial drug artesunate ameliorates oxidative lung damage in experimental allergic asthma

10.1016/j.freeradbiomed.2012.05.021Free Radical Biology and Medicine533498-507FRBM

Serum Metabolomics Reveals Serotonin as a Predictor of Severe Dengue in the Early Phase of Dengue Fever

10.1371/journal.pntd.0004607PLoS Neglected Tropical Diseases104e000460

In situ analysis of 8-Oxo-7,8-dihydro-2′-deoxyguanosine oxidation reveals sequence- and agent-specific damage spectra

Guanine is a major target for oxidation in DNA, with 8-oxo-7,8-dihydro- 2′-deoxyguanosine (8-oxodG) as a major product. 8-oxodG is itself significantly more susceptible to oxidation than guanine, with the resulting damage consisting of more than 10 different products. This complexity has hampered efforts to understand the determinants of biologically relevant DNA oxidation chemistry. To address this problem, we have developed a high mass accuracy mass spectrometric method to quantify oxidation products arising site specifically in DNA. We applied this method to quantify the role of sequence context in defining the spectrum of damage products arising from oxidation of 8-oxodG by two oxidants: nitrosoperoxycarbonate (ONOOCO2 -), a macrophage-derived chemical mediator of inflammation, and the classical one-electron oxidant, riboflavin-mediated photooxidation. The results reveal the predominance of dehydroguanidinohydantoin (DGh) in 8-oxodG oxidation by both oxidants. While the relative quantities of 8-oxodG oxidation products arising from ONOOCO2 - did not vary as a function of sequence context, products of riboflavin-mediated photooxidation of 8-oxodG were highly sequence dependent. Several of the 8-oxodG oxidation products underwent hydrolytic conversion to new products with half-lives of 2-7 h. The results have implications for understanding the chemistry of DNA oxidation and the biological response to the damage, with DNA damage recognition and repair systems faced with a complex and dynamic set of damage targets. © 2012 American Chemical Society

Serum metabolomics investigation of humanized mouse model of dengue virus infection

10.1128/JVI.00386-17Journal of Virology9114e00386-1