24 research outputs found
Pretreatment of Paper Sludge with Rumen Fluid to Enhance Biogas Production
Poster Presentation
Development of Optimal Continuous Culture Method for Rumen Fluid Using Ferrite Particles
Poster Session
Characterizing the Key Factors in Potential Suppressive Anaerobic Digester Effluent on Bacterial Wilt Disease (Ralstonia solanacearum)
Poster Session
High-resolution imaging of complex aortic plaques in ischemic stroke patients using 3.0 Tesla MRI with VISTA
Discrimination of Dormant and Active Hematopoietic Stem Cells by G<sub>0</sub> Marker Reveals Dormancy Regulation by Cytoplasmic Calcium
Quiescent hematopoietic stem cells (HSCs) are typically dormant, and only a few quiescent HSCs are active. The relationship between “dormant” and “active” HSCs remains unresolved. Here we generate a G0 marker (G0M) mouse line that visualizes quiescent cells and identify a small population of active HSCs (G0Mlow), which are distinct from dormant HSCs (G0Mhigh), within the conventional quiescent HSC fraction. Single-cell RNA-seq analyses show that the gene expression profiles of these populations are nearly identical but differ in their Cdk4/6 activity. Furthermore, high-throughput small-molecule screening reveals that high concentrations of cytoplasmic calcium ([Ca2+]c) are linked to dormancy of HSCs. These findings indicate that G0M separates dormant and active adult HSCs, which are regulated by Cdk4/6 and [Ca2+]c. This G0M mouse line represents a useful resource for investigating physiologically important stem cell subpopulations
Discrimination of Dormant and Active Hematopoietic Stem Cells by G<sub>0</sub> Marker Reveals Dormancy Regulation by Cytoplasmic Calcium
Quiescent hematopoietic stem cells (HSCs) are typically dormant, and only a few quiescent HSCs are active. The relationship between “dormant” and “active” HSCs remains unresolved. Here we generate a G0 marker (G0M) mouse line that visualizes quiescent cells and identify a small population of active HSCs (G0Mlow), which are distinct from dormant HSCs (G0Mhigh), within the conventional quiescent HSC fraction. Single-cell RNA-seq analyses show that the gene expression profiles of these populations are nearly identical but differ in their Cdk4/6 activity. Furthermore, high-throughput small-molecule screening reveals that high concentrations of cytoplasmic calcium ([Ca2+]c) are linked to dormancy of HSCs. These findings indicate that G0M separates dormant and active adult HSCs, which are regulated by Cdk4/6 and [Ca2+]c. This G0M mouse line represents a useful resource for investigating physiologically important stem cell subpopulations