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Not AvailableThe lack of shrimp cell lines and difficulty in establishing shrimp cell culture systems, with an appropriate medium is a major concern in the aquaculture sector. The present study attempts to address this issue by developing an in vitro cell culture system from various tissues (hemocytes, heart, lymphoid tissue, hepatopancreas, gill, eye stalk, and muscle) of Penaeus vannamei (P.vannamei) using commercially available L-15 medium. The cell culture medium was formulated using five different media such as HBSCM-1, HBSCM-2, HBSCM-3, HBSCM-4, and HBSCM-5 containing L-proline and glucose with fetal bovine serum (FBS) supplements. Among the different media used, the HBSCM-5 medium with supplements showed good attachment and proliferation of cells with fibroblast-like, epithelioid, round, and adherent cell morphology in hemocyte culture. The same medium was further screened using different tissues to enhance the cell growth. The hemocytes, heart, and lymphoid tissue cells were passaged five times and maintained up to 20 days. Hepatopancreas and gill cells initially showed good morphological features and survived for more than ten days following subculture cells. Eye stalks and muscle cells perished within five days and did not show any unique morphology. The primary hemocyte cells were subjected to species identification, using cytochrome oxidase subunit I (COI) gene. To assess the primary hemocyte cell culture, cells were used for in vitro propagation of white spot syndrome virus (WSSV) and confirmed by the conventional polymerase chain reaction (PCR). Similarly, the primary cells were treated with bacterial extracellular products (ECPs) from Vibrio parahaemolyticus and Vibrio harveyi, to evaluate the cytotoxicity.Not Availabl

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Not AvailableTunas are commercially important fishes, widely distributed throughout the tropical and temperate waters. During processing and development of various value added products from tuna, large quantity of solid waste is generated in the form of viscera, gills, dark muscle, head, bone and skin. In this study, waste generated from tuna processing was converted into a liquid protein source by ensilation and its performance as a novel protein source for pigs was evaluated. Feed was prepared by mixing rice bran and tuna waste silage in 7:3 ratio and drying at 60oC in an electrical drier for 12 h. The feed has crude protein 20%, crude fat 22%, ash content 8% and moisture content of 7%. The feed was found to be rich in essential dietary amino acids (lysine, tryptophan, threonine and methionine) and fatty acid (oleic and linoleic acid). Feeding trials on thirty six weaned large white Yorkshire piglets by incorporating tuna waste silage for a period of 104 days showed that this can be used as an efficient feed for pigs.Not Availabl

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Not AvailableTunas are commercially important fishes, widely distributed throughout the tropical and temperate waters. During processing and development of various value added products from tuna, large quantity of solid waste is generated in the form of viscera, gills, dark muscle, head, bone and skin. In this study, waste generated from tuna processing was converted into a liquid protein source by ensilation and its performance as a novel protein source for pigs was evaluated. Feed was prepared by mixing rice bran and tuna waste silage in 7:3 ratio and drying at 60oC in an electrical drier for 12 h. The feed has crude protein 20%, crude fat 22%, ash content 8% and moisture content of 7%. The feed was found to be rich in essential dietary amino acids (lysine, tryptophan, threonine and methionine) and fatty acid (oleic and linoleic acid). Feeding trials on thirty six weaned large white Yorkshire piglets by incorporating tuna waste silage for a period of 104 days showed that this can be used as an efficient feed for pigs.Not Availabl