13 research outputs found
Solid-Phase PCR in Microwells: Effects of Linker Length and Composition on Tethering, Hybridization, and Extension
Identifying regions of the wheat genome controlling seed development by mapping expression quantitative trait loci
GLOBALIZATION'S IMPACT ONSTATE AND LOCAL POLICY: The Rise of Regional Cluster-Based Economic Development Strategies -super-1
We propose proactive policies which allow state and local governments to participate in the global economy, offering opportunities and venues for effective development. We first develop the argument that the changing economic conditions associated with globalization call for a new approach to state-local economic development policy. We then outline three assumptions which are critical to drive successful state and local economic development policy in a global environment. The major part of the analysis describes this new strategy of "regional cluster-based economic development," drawing extensively upon a case study of Cleveland's economic revitalization. Copyright 2001 by The Policy Studies Organization.
The 2006 NESCent phyloinformatics hackathon: A field report
10.1177/117693430700300016Evolutionary Bioinformatics3287-29
Mapping quantitative trait loci in inbred backcross lines of Lycopersicon pimpinellifolium
The genomic organization of retrotransposons in Brassica oleracea
We have investigated the copy numbers and genomic organization of five representative reverse transcriptase domains from retrotransposons in Brassica oleracea. Two non-homologous Pseudoviridae (Ty1/copia-like) elements, two Metaviridae (Ty3/gypsy-like) elements (one related to the Athila family) and one Retroposinae (LINE) element were hybridized to a gridded BAC library, “BoB”. The results indicated that the individual LTR retrotransposons (copia and gypsy-like) were represented by between 90 and 320 copies in the haploid genome, with only evidence of a single location for the LINE. Sequence analysis of the same elements against genome survey sequence gave estimates of between 60 and 570, but no LINE was found. There was minimal evidence for clustering between any of these retroelements: only half the randomly expected number of BACs hybridized to both LTR-retrotransposon families. Fluorescent in situ hybridization showed that each of the retroelements had a characteristic genomic distribution. Our results suggest there are preferential sites and perhaps control mechanisms for the insertion or excision of different retrotransposon groups