Effects of Eugenol and MS-222 Anaesthesia on Siberian Sturgeon Acipenser baerii Brandt

The aim of the present study was to assess changes induced in the organism of Siberian sturgeon (Acipenser baerii) by eugenol and MS-222 anaesthesia on the basis of haematological indices, biochemical blood profile values and histological examinations. The haematological and biochemical indices were assessed in fish immediately and 24 h after anaesthesia. The results showed that despite no mortality occurred after anaesthesia in both 125 mg l-1 of MS-222 and 0.075 ml l-1 of eugenol, these chemical substances severely influenced the constituents of Siberian sturgeon blood and resulted in some histological changes in the gills and liver. Both eugenol and MS-222 anaesthesia caused erythrocyte swelling and haemolysis. The severe depletion of leukocyte number occurred 24 h after both eugenol and MS-222 anaesthesia (mainly due to depletion of lymphocyte, neutrophil segments and eosinophil fractions). Total protein, total globulin, triacylglycerol concentration and alanine aminotransferase activity in blood plasma was significantly elevated (p 2+, inorganic phosphate, NH3 and alkaline phosphatase activity were significantly decreased (p -1) and eugenol (0.075 ml l-1) does not cause irreversible damage in Siberian sturgeon

ANGUILLICOLOSIS OF THE EUROPEAN EEL — STILL ACTUAL PROBLEM

Some notes on anguillicolosis, parasitosis caused by Asiatic nematode Anguillicola living in the swimbladder of susceptible Anguilla anguilla L. are given: the distribution and spreading, the life cycle of ovoviviparous A. crassus and its ecology, pathogenicity for European eel and preventive measures

The leukocyte system in rainbow trout, Salmo gairdneri Rich. affected by prolonged subacute phenol intoxication

The paper gives a quantitative and qualitative analysis of leukocytes in the peripheral blood, spleen, and pronephros of rainbow trout affected by phenol intoxication (2 mg phenol dm-3 applied for 2 months in winter)

Erythrocyte system of rainbow trout, Salmo gairdneri Rich. affected by prolonged subacute phenol intoxication

Basic erythrocyte indices, erythrocyte osmotic resistance as well as quantitative and qualitative analysis of the erythrocyte pattern in the peripheral blood, spleen and kidney of rainbow trout affected by phenol intoxication (2 mg dm-3 doses applied for 2 months in winter) are presented

Selected indices of immunologic resistance in rainbow trout (Salmo gairdneri Rich.) during prolonged, subacute phenolic intoxication

Blastic transformation of the lymphocytes in peripheral blood and spleen, analysis of immunocompetent cells in the blood and haematopoietic organs of rainbow trout after phenolic intoxication (2 mg/dm3, 2 months, winter)

Fish digeneans from the Seven Islands ornithological reserve at OĹ›win Lake. Part II. The eyeflukes–Diplostomum spp. and Tylodelphys lavata(von Nordmann, 1832)

Background. Aparasitological survey was carried out in a shallow, eutrophic Oświn Lake (north-eastern Poland), within the Seven Islands ornithological reserve. We intended to compare the occurrence of eyeflukes in fish from two, distinctly demarcated pools of the lake, differing in environmental conditions. The effect of fish size and sampling season on the infection parameters was analysed and the present state of the parasite faunas was compared to the literature data. Materials and methods. Within 1998 and 1999, a total of 1091 fishes representing 8 dominant species was examined. Samples were collected four times a year (in May, July, August, and October) simultaneously from the eastern- and western parts of the lake. Results. Diplostomum spp. occurred in all the fish examined, numerously in roach, rudd, white bream, and carp bream. The parasite was less abundant in northern pike, crucian carp, and European perch. Tylodelphys clavata was found in roach and it sporadically occurred in northern pike, rudd, white bream, carp bream, and European perch. The infection rates of rudd and white bream in the eastern part of the lake were significantly higher than the respective values from the western part. The infection with both eyeflukes did not correlate with the fish length. The infection of roach, rudd, white bream, and carp bream with Diplostomum spp. and roach with T. clavata varied significantly in different experimental periods. Conclusion. The eyeflukes as well as P. cuticola were sensitive indicators, reflecting the environmental pressure-both, spatial differences in ecological conditions within the same water body and long-term alterations. Diplostomum spp. did not accumulate with the age of fish. No seasonality was found for Diplostomum spp. or Tylodelphys clavata. Locationdependent occurrence of Diplostomum spp. in rudd and white bream indicated a limited fish interchange between the two pools of the lake

The occurrence of parasites in bream (Abramis brama L.), roach (Rutilus rutilus L.) and pike perch (Stizostedion lucioperca L.) in the Wloclawek Dam Reservoir on the river Vistula

Fish caught in the Włocławek Dam Reservoir during the years 1982-83 in April, July and August were parasitologically, anatomopathologically and histopathologically examined (cf. tab. 1). For histopathological examination fresh and stained permanent slides whenever necessary were made. In the studied fish parasitic protozoans (Flagellata, Sporozoa and Ciliata) as well as metazoans (flukes, tapeworms, nematods, crustaceans and, sporadically, molluscan glochidia) were found to occur. Within the first group the most important with respect to the extensity, and in some months also the intensity of infection were the Sporozoa of the genus Pleistophora (Microspora), then Myxobolus mülleri (Cnidosporidia) and Rhabdospora thelohani, within the second group - metacercariae of Diplostomum spathaceum and Tylodelphys clavatum. The parasite fauna of the bream and roach does not show any significant qualitative or quantitative differences, while in the parasite fauna of the pike perch - beside the parasites typical of this species - the parasites found also in the bream and roach i.e. in the cyprinids, dominate both qualitatively and quantitatively. Beside specific changes, characteristic of the infection with the parasitic metazoans, a higher intensity of invasion, especially in the case of Sporozoa, is accompanied by pathomorphological changes such as tissue damage, necrosis, blood supply disturbance swelling, inflammatoiry reaction with cell infiltrations and general emaciation. Such changes were not found in the great part of the fish stock, with a low intensity of infection

Sphaerospora renicola and S. molnari in Poland and spring sphaerosporosis of carp

Two myxosporean species: Sphaerospora renicola Dyková et Lom, 1982 and S. molnari Lom, Dyková, Pavlaskova et Grupcheva, 1983 were recorded for the first time in Poland. They were found in young carp after first overwintering in cold- and warm-water ponds. Localization of different developmental stages in carp tissues is described. Differences in frequency of particular developmental stages were noticed between the cold and warm ponds. In fish taken from cold ponds spores occurred rarely. In fish from the warm pond spores were abundant, but extrasporogonic stages were practically undetectable. Symptoms of spring sphaerosporosis are described. They were mainly connected with the presence and release of spores, and were much more serious in carp from the warm pond

Interesting case of pathological changes in rainbow trout Oncorhynchus mykiss (Walbaum) probably caused by Myxosporea

The causative agents of health problems of rainbow trout from a fish farm were investigated by parasitological and anatomo-pathological study of affected fishes. The anatomo-pathological changes in gills, swim bladder, kidney, and especially the deformations of backbone are described. The numerous trophozoites of unidentified species of Myxosporea, occurring in all damaged tissues are suspected to be a causative agent of the disease and mortality in the fish farm under study