Assessment of the Application of Erythrocytal Diagnosticum (Lyophilizate) in Detecting Tularemia Agent in Natural Foci

Tularemia is a zoonotic disease with a wide geographical dissemination, and its causative agent Francisella tularensis can be used as a bioterrorism agent. The aim of the study was to evaluate the use of a set of reagents “Erythrocytic immunoglobulin dry tularemia diagnosticum” (“DET-Ig”) with the help of control test strains and field material from natural tularemia foci. Materials and methods. Using the introduced erythrocyte diagnosticum, we studied the decontaminated cultures of test strains (F. tularensis Miura, F. tularensis 55, F. tularensis Schu, F. tularensis 15 NIIEG, Brucella abortus 544, B. melitensis 16-M, B. suis 1330, and Yersinia enterocolitica 64, Y. enterocolitica 178, Y. enterocolitica 383) and environmental samples suspected of containing F. tularensis. Results and discussion. It has been proven that the developed diagnosticum is specific, sensitive, and easy to use for routine diagnostics of tularemia. In the course of laboratory tests of the experimental series of the DET-Ig reagent kit, the possibility of qualitative determination of the tularemia agent in bacterial cultures, biological material and environmental samples in the reaction of indirect hemagglutination was demonstrated. Comparison of the results of use of erythrocyte diagnosticum in liquid and lyophilized forms showed the advantages of drugs after lyophilization: the possibility of transportation and long-term storage at any temperature conditions in various climatic conditions; the setting of the reaction is possible without the use of special diluents. The guaranteed storage term is set for two years (observation period). The results obtained indicate the prospects of introducing the developed drug into healthcare practice

Validation of Technological Process of Production of Liquid Brucellosis Diagnosticum for Agglutination Reaction, Suspension for Diagnostic Purposes

Presented are the results of validation of technological process of production of brucellosis diagnosticu

Serological methods for detection of the causative agent of tularemia and their evaluation

Aim. A comparative study of serological methods for the detection of the causative agent of tularemia and their evaluation. Materials and methods. We used experimental diagnostic kits and test systems for the production of serological methods: indirect hemagglutination reaction (RGA); the reaction immunofluorescence (RIF); enzyme immunoassay (ELISA) using traditional microplate; IFA after selective concentration of the pathogen of tularemia in magnoimmunosorbents (MIS); microgravimetric analysis (MGA) based on piezoresistors (SP) and surface plasmon resonance (SPR). The experiments were carried out with homologous strains of tularemia microbe (test strains) and with strains of heterologous microorganisms in model experiments on tap water contaminated with different concentrations of the pathogen. Results. The parameters of each diagnostic method are determined and evaluated according to the following indicators: sensitivity (when working with pure cultures (test strains), contaminated samples of large volumes), specificity, time of setting and taking into account the results, informativeness, determining the modes of setting and accounting. Conclusion. The above diagnostic methods have their advantages and disadvantages. Therefore, when choosing a method, the researcher should be guided by the goals pursued. So, for screening studies it is advisable to carry out the formulation of ELISA, RIF, RGA, in identifying the pathogen in large volumes and contaminated samples, the effective use of selective concentration on MIS followed by the formulation of ELISA, to identify small amounts of samples and take into account the reaction in real time, it is possible to use MGA and SPR

EPIDEMIOLOGIC SITUATION BY NATURAL-FOCI INFECTIONS IN THE CRIMEA FEDERAL DISTRICT IN 2014 - 2015

Aim. Analysis of epidemic manifestations of natural-foci infections (NFI), clarification of spectrum of their causative agents, determination of epizootic activity of natural foci in the Crimea Federal District (KFD). Materials and methods. Epizootologic examination of 10 administrative districts of KDF was carried out. 291 pools (2705 specimens) of ixodes ticks and 283 samples of organs of small mammals were studied by PCR method for the presence of DNA/RNA of causative agents of a number of NFI. Results. Morbidity by NFI in KFD was registered by 6 nosologies: Lyme borreliosis, Marseilles fever, leptospirosis, tularemia, intestine yersiniosis and tick-borne viral encephalitis, wherein, transmissive infections made up 91.6%. Circulation of causative agents of Crimea hemorrhagic fever, Q fever, group of tick-borne spotted fever, Lyme borreliosis, human granulocytic anaplasmosis, human monocytic ehrlichiosis, hemorrhagic fever with renal syndrome, West Nile fever, tularemia and leptospirosis was established. Conclusion. Due to activity of natural foci of NFI further monitoring of epidemiologic and epizootologic manifestations of these infections in the Crimea, including using genetic methods of analysis, is necessary for ensuring sanitary-epidemiologic welfare of KFD population