141 research outputs found
Templated Synthesis of Peptide Nucleic Acids via Sequence-Selective Base-Filling Reactions
The templated synthesis of nucleic acids has previously been achieved through the backbone ligation of preformed nucleotide monomers or oligomers. In contrast, here we demonstrate templated nucleic acid synthesis using a base-filling approach in which individual bases are added to abasic sites of a peptide nucleic acid (PNA). Because nucleobase substrates in this approach are not self-reactive, a base-filling approach may reduce the formation of nontemplated reaction products. Using either reductive amination or amine acylation chemistries, we observed efficient and selective addition of each of the four nucleobases to an abasic site in the middle of the PNA strand. We also describe the addition of single nucleobases to the end of a PNA strand through base filling, as well as the tandem addition of two bases to the middle of the PNA strand. These findings represent an experimental foundation for nonenzymatic information transfer through base filling.Chemistry and Chemical Biolog
Issues of geologically-focused situational awareness in robotic planetary missions: lessons from an analogue mission at Mistastin Lake impact structure, Labrador, Canada
Remote robotic data provides different information than that obtained from immersion in the field. This significantly affects the geological situational awareness experienced by members of a mission control science team. In order to optimize science return from planetary robotic missions, these limitations must be understood and their effects mitigated to fully leverage the field experience of scientists at mission control.
Results from a 13-day analogue deployment at the Mistastin Lake impact structure in Labrador, Canada suggest that scale, relief, geological detail, and time are intertwined issues that impact the mission control science team‟s effectiveness in interpreting the geology of an area. These issues are evaluated and several mitigation options are suggested. Scale was found to be difficult to interpret without the reference of known objects, even when numerical scale data were available. For this reason, embedding intuitive scale-indicating features into image data is recommended. Since relief is not conveyed in 2D images, both 3D data and observations from
multiple angles are required. Furthermore, the 3D data must be observed in animation or as anaglyphs, since without such assistance much of the relief information in 3D data is not
communicated. Geological detail may also be missed due to the time required to collect, analyze, and request data.
We also suggest that these issues can be addressed, in part, by an improved understanding of the operational time costs and benefits of scientific data collection. Robotic activities operate on inherently slow time-scales. This fact needs to be embraced and accommodated. Instead of focusing too quickly on the details of a target of interest, thereby potentially minimizing science return, time should be allocated at first to more broad data collection at that target, including
preliminary surveys, multiple observations from various vantage points, and progressively smaller scale of focus. This operational model more closely follows techniques employed by
field geologists and is fundamental to the geologic interpretation of an area. Even so, an operational time cost/benefit analyses should be carefully considered in each situation, to determine when such comprehensive data collection would maximize the science return.
Finally, it should be recognized that analogue deployments cannot faithfully model the time scales of robotic planetary missions. Analogue missions are limited by the difficulty and expense of fieldwork. Thus, analogue deployments should focus on smaller aspects of robotic missions and test components in a modular way (e.g., dropping communications constraints, limiting mission scope, focusing on a specific problem, spreading the mission over several field seasons,
etc.)
Aerodynamic Computations Using the Convective-Upstream Split-Pressure Scheme with Local Preconditioning
Effects of Rain on Traffic Operations on Florida Freeways
Although the correlation between traffic variables and weather appears to be intuitive, quantifying the effects that weather, especially rain, has on driver response in travel speeds and traffic demands is needed to evaluate practical aspects of traffic operations. Previous studies have researched driver responses to inclement weather on freeways located in northern regions of the United States and Canada. However, driver familiarity with local weather conditions is a factor that should be considered in determining inclement weather effects on traffic variables. The focus of this research was to examine driver response to rain precipitation on freeways located in the southeastern regions of the United States to determine whether results from previous studies were general Indicators or location specific in nature. To study the impacts of rain precipitation on hourly mean speeds and traffic volumes, hourly weather data and traffic sensor data were collected for two freeway segments in Jacksonville, Florida. The study investigated conditions such as wet versus dry (rain or no rain) and dry versus rain intensity (no rain or light, moderate, or heavy rain) for each segment. The results indicated that mean travel speeds decreased during rainfall events and speed reductions increased with increasing rain intensity. Reductions found for light rainfall events were within the range of previous studies; however, speed reductions during moderate to heavy rains varied widely. The results also indicated that the hour of the day was a factor in the degree of motorists\u27 speed reduction. Traffic volumes also declined during rainy conditions, with significant reductions during peak hours
Effects of the tyre dimension on the energy expenditure within different driving cycles and vehicle concepts
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