MOLECULAR GENETIC ANALYSIS OF INFECTION AGENTS OF FARM ANIMALS ANAPLASMOSIS ON THE TERRITORY OF WESTERN AND EASTERN SIBERIA

A total of 452 blood samples of cattle, sheep and goat collected in different regions of Altai Republic, Altai region, Novosibirsk and Irkutsk regions were examined on the presence of Anaplasma DNA using nested PCR with subsequent sequencing of PCR fragments. Anaplasma DNA was found in all examined blood samples of goat, 75.2 % samples of sheep, and 49.3 % samples of cattle. A molecular genetic analysis has demonstrated that intraerythrocytic Anaplasma ovis circulates in goat and sheep blood, while an intraerythrocytic Anaplasma sp. Omsk and a new intraerythrocytic Anaplasma sp. Sibl22, which cannot be attributed to any known species, circulate in cattle blood. In addition to intraerythrocytic Anaplasma, DNA of monocytic Anaplasma bovis was found in one blood sample of cattle

INTRAVITREAL INTRODUCTION OF LUCENTIS AND TRANS-SCLERAL CYCLOPHOTOCOAGULATION IN THE TREATMENT OF NEOVASCULAR GLAUCOMA IN DIABETES MELLITUS

We analyzed the results of surgical treatment of 42 patients (42 eyes) with neovascular glaucoma on the background of proliferative diabetic retinopathy. Intravitreal injections of anti-VEGF-drugs allow to remove newly formed vessels in anterior chamber angle, to reduce intraocular pressure, to avoid complications in patients with neovascular glaucoma and the closed anterior chamber angle of the eye. The second stage of the treatment can be laser surgery. This approach helped to reduce intraocular pressure from 28.9 ± 0.9 to 18.5 ± 0.9 mmHg on the background of medical treatment (t > 2.0; p 2.0; p 0.05)

NEW EVALUATION METHOD FOR EFFICACY OF HYPOTENSIVE TREATMENT WITH ACE INHIBITORS

The work, using Enam as an example, provides a new original evaluation method for hypotensive action of drugs. The method is based upon primary analysis of daily blood pressure monitoring data with subsequent transformation into a graphic form of BP values likelihood distribution over a plane. The authors called the image obtained in this manner hypertension areal. The authors distinguish a core and a peripheral part of hypertension areal, proposing an evaluation method for many characteristic parameters. Hypotensive action is evaluated by changes in areal square over time. Patients receiving Enam were divided into 3 groups depending on the extent of hypotensive effect, by means of a neural network. Hypotensive effect is characterized based on the proposed standpoints for each group

Anomalously deep BSR related to a transient state of the gas hydrate system in the western Black Sea

A comprehensive characterization of the gas hydrate system offshore the western Black Sea was performed through an integrated analysis of geophysical data. We detected the Bottom Simulating Reflector (BSR), which marks, in this area, the base of gas hydrate stability. The observed BSR depth does not fit the theoretical steady-state base of gas hydrate stability zone (BGHSZ). We show that the disparity between the BSR and predicted BGHSZ is the result of a transient state of the hydrate system due to the ongoing re-equilibrium since the Last Glacial Maximum. When gas hydrates are brought outside the stability zone due to changes in temperature and sea level, their dissociation generates an increase in interstitial pore pressure. This process is favorable to the re-crystallization of gas hydrates and delays the upward migration of the hydrate stability zone explaining the anomalously deep BSR. The BSR depth, which is commonly used to derive geothermal gradient values by assuming steady state conditions, is used here to derive the maximum excess pore pressure at the base of the gas hydrate stability zone. Derived excess pore pressure values of 1-2 MPa are probably the result of the low permeability of hydrate-bearing sediments. Higher pore pressure values derived at the location of a fault system could cause hydro-fracturing enabling the free gas to cross the gas hydrate stability zone and emerge at the seafloor, forming the flares observed in close vicinity to where the shallow gas hydrates were sampled