26 research outputs found
Molecular diagnostics using analytical immuno high performance liquid affinity chromatography
No full textAnalytical immuno high performance liquid affinity chromatography (analytical immuno HPLAC) was evaluated as a molecular diagnostic tool. Antibodies raised in rabbits against bovine neurophysin II were immobilized through Protein A crosslinking onto coated silica. Interaction of immobilized antibody with mobile antigen was characterized by zonal and frontal elutions of 14C-labeled bovine neurophysin II under isocratic, nondenaturing conditions. The chromatographic behavior shows that analytical immuno HPLAC with immobilized antibodies can be used to detect the number and functional nature of matrix-interacting antigens in mixtures, thus providing a quantitative chromatographic technology for "antigen mapping.
Two-stage model of incorporation of seamount and oceanic blocks into sedimentary melange: Geochemical and biostratigraphic constraints in Jurassic Chichibu accretionary complex, Shikoku, Japan
No full textMolecular diagnostics using analytical immuno high performance liquid affinity chromatography
No full textIdentification and immunohistochemical localization of various bovine pancreatic trypsin inhibitor-isoforms in bovine pituitary gland
No full textMicrofacies and depositional setting of the Upper Triassic mid-oceanic atoll-type carbonates of the Sambosan Accretionary Complex (southern Kyushu, Japan)
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CHEMICAL, PHYSICAL, AND BIOCHEMICAL CONCEPTS IN ISOLATION AND PURIFICATION OF PROTEINS *
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Structure-activity Relationships of Neoechinulin A Analogues with Cytoprotection against Peroxynitrite-induced PC12 Cell Death
No full textSynthesis and Biological Activities of Neoechinulin A Derivatives: New Aspects of Structure–Activity Relationships for Neoechinulin A
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